Protease-producing strain exiguobacterium sp. and applications thereof

A protease-producing technology of the genus Microbacterium, which is applied in the direction of bacteria, microorganisms, hydrolytic enzymes, etc., can solve the problems that the protease-producing Microbacterium genus has not yet been reported, and achieve the effects of easy cultivation, stable genetic properties, and simple culture conditions

Active Publication Date: 2013-09-25
SOUTH CHINA UNIV OF TECH
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] The protease-producing marine bacteria that have been discovered so far mainly include Pseudomonas, Flavobacterium, Bacillus, Vibrio, and Brevibacterium oxidans, etc., and the deep-sea source of protease-producing Exiguobacterium has not been reported yet.

Method used

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  • Protease-producing strain exiguobacterium sp. and applications thereof
  • Protease-producing strain exiguobacterium sp. and applications thereof
  • Protease-producing strain exiguobacterium sp. and applications thereof

Examples

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Embodiment Construction

[0014] Enrichment and seed medium: peptone 5g, yeast powder 1g, artificial seawater 1L, pH 7.4-7.6.

[0015] Casein medium: 10g casein, 3g beef extract powder, 2g potassium dihydrogen phosphate, 15g agar powder, 1L artificial seawater, pH 7.4 or so.

[0016] Fermentation medium: glycerol 3g, glucose 5g, yeast powder 10g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.3g, ammonium sulfate 1g, calcium chloride 1g, sea salt 10g, pH7.2 or so.

[0017] Isolation and screening of protease-producing CGMCC No.7387

[0018] Enrichment culture: Weigh 2g of deep-sea mud sample into a 50mL sterilized centrifuge tube, add 10mL of sterile distilled water, shake in a shaker for 20min to fully disperse, after standing still, take 1mL of the supernatant in the enrichment culture ( 50 mL / 250mL), 37°C, 120rpm, cultured for 48h.

[0019] Primary screening: Add 1mL of the enriched culture solution into 9mL sterile physiological water, and dilute to different gradients (10 -1 -10 -7 )...

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Abstract

The invention relates to a protease-producing strain exiguobacterium sp. and applications thereof. A protease high-producing strain is screened out from deep sea muds through first screening with casein plates and second screening with shake flask fermentation, and identified as exiguobacterium sp. The protease-producing strain exiguobacterium sp. is collected in China General Microbiological Culture Collection Center on March 29, 2013. The collection number is CGMCC No. 7387. The protease-producing conditions are optimized by single factor experiments. The protease-producing strain exiguobacterium sp. is easy to culture, and is advantaged by simple culture conditions and stable hereditary properties.

Description

technical field [0001] The present invention relates to the protease producing bacterial strain of deep-sea source, be specifically related to Microbacterium genus ( Exiguobacterium sp. ) SWJS2 and the method for producing protease by liquid fermentation thereof. Background technique [0002] Protease is a type of enzyme that decomposes protein peptide bonds. It is closely related to human life and accounts for more than 60% of the total sales of enzyme preparations in the world. However, its wider application is restricted by production costs and enzymatic properties. Therefore, new proteases are explored Resources and strains with high protease production have been the focus of researchers. With the increasing diversity and specific demands of human beings on biological resources, existing terrestrial resources cannot meet human demands for new resources while being continuously excavated. marine living resources. The marine environment generally has the characteristics...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/52C12R1/01
Inventor 赵谋明崔春雷芬芬苏国万
Owner SOUTH CHINA UNIV OF TECH
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