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Preparation method of high strength double-network antibacterial biological hydrogel

A hydrogel and dual network technology, applied in the field of biomedical hydrogel synthesis, can solve the problems of limited hydrogel application, poor mechanical properties, easy to be infected by bacteria, etc., achieving good mechanical properties, improved toughness, The effect of mild reaction conditions

Active Publication Date: 2017-05-31
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, good biocompatibility makes it easy to be infected by bacteria, and the structure of biological tissue makes the mechanical properties poor, which limits the application of hydrogels.

Method used

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  • Preparation method of high strength double-network antibacterial biological hydrogel
  • Preparation method of high strength double-network antibacterial biological hydrogel
  • Preparation method of high strength double-network antibacterial biological hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033](1) Weigh 5g of gelatin into 50ml of phosphate buffer solution, stir and dissolve at 40°C; take 2ml of methacrylic anhydride, slowly add it dropwise to the above solution, and stir for 2 hours; pour the reacted mixture into 40 In the phosphate buffer solution at ℃, stir for 2 minutes to terminate the reaction; use a dialysis bag to pack the above liquid, and dialyze in deionized water at 40 ℃ for 6 days; divide the dialyzed solution with a centrifuge tube and centrifuge to collect the supernatant , stored in an ultra-low temperature refrigerator for 24 hours, and then freeze-dried in a freeze dryer to obtain a high-purity methacrylic anhydride gelatin sample.

[0034] (2) Weigh 3g of histidine and dissolve it in 7ml of sodium hydroxide solution, pass through nitrogen and dissolve in an ice bath; take 1.5ml of acryloyl chloride, and slowly add it dropwise to the above solution under nitrogen atmosphere and dark conditions ; After maintaining the reaction at 0°C for 30 min...

Embodiment 2

[0040] (1) Weigh 5g of gelatin into 50ml of phosphate buffer solution, stir and dissolve at 50°C; take 3ml of methacrylic anhydride, slowly add it dropwise to the above solution, stir and react for 3h; pour the reacted mixture into 50 In the phosphate buffer solution at ℃, stir for 2 minutes to stop the reaction; use a dialysis bag to pack the above liquid, and dialyze it in deionized water at 50 ℃ for 8 days; divide the dialyzed solution with a centrifuge tube and centrifuge to collect the supernatant , stored in an ultra-low temperature refrigerator for 24 hours, and then freeze-dried in a freeze dryer to obtain a high-purity methacrylic anhydride gelatin sample.

[0041] (2) Weigh 3g of histidine and dissolve it in 7ml of sodium hydroxide solution, pass through nitrogen and dissolve in an ice bath; take 1.7ml of acryloyl chloride, and slowly add it dropwise to the above solution under nitrogen atmosphere and dark conditions ; After keeping the reaction at 2°C for 40 minutes...

Embodiment 3

[0048] (1) Weigh 5g of gelatin into 50ml of phosphate buffer solution, stir and dissolve at 60°C; take 4ml of methacrylic anhydride, slowly add it dropwise to the above solution, and stir for 4 hours; pour the reacted mixture into 60 Phosphate buffer solution at ℃, stirred for 2 min, and the reaction was terminated; the above liquid was divided into dialysis bags, and dialyzed in deionized water at 60 °C for 10 days; the dialyzed solution was divided into centrifuge tubes and centrifuged, and the supernatant was collected. Freeze in an ultra-low temperature refrigerator for 24 hours, and then freeze-dry in a freeze dryer to obtain a high-purity methacrylic anhydride gelatin sample.

[0049] (2) Weigh 3g of histidine and dissolve it in 7ml of sodium hydroxide solution, pass through nitrogen and dissolve in an ice bath; take 1.8ml of acryloyl chloride, and slowly add it dropwise to the above solution under nitrogen atmosphere and dark conditions ; After keeping the reaction at 0...

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Abstract

The invention discloses a preparation method of high strength double-network antibacterial biological hydrogel. The preparation method comprises (1) mixing modified gelatin and modified histidine, dissolving the mixture in a phosphate buffer solution, and adding a photoinitiator into the solution to cause polymerization under the ultraviolet point light source so that gelatin hydrogel having an imidazole active site is obtained, and (2) soaking the gelatin hydrogel having an imidazole active site in a divalent metal ion salt solution, and then washing the gelatin hydrogel through sterile water to obtain the high strength double-network antibacterial biological hydrogel. The preparation method has the advantages of mild reaction conditions, strong operability and process controllability. The high strength double-network antibacterial biological hydrogel has good mechanical properties, rigidity and toughness, has escherichia coli and staphylococcus aureus killing rates of 99.9% and has good biocompatibility of the base hydrogel.

Description

technical field [0001] The invention relates to the field of biomedical hydrogel synthesis, in particular to a preparation method of a high-strength double-network antibacterial biohydrogel. Background technique [0002] Hydrogel is a three-dimensional polymer material capable of absorbing and storing a large amount of water. This excellent performance is attributed to the fact that hydrogel has a hydrophilic group and a cross-linked three-dimensional network skeleton structure. Due to the good hydrophilicity, permeability and biocompatibility of the hydrogel, as well as the low coefficient of friction performance (Jason W. Nichol, Sandeep T. Koshy, et, al.Cell-laden micro-engineered gelatin methacrylate hydrogels. Bio -materials 31, 2010, 5536-5544), making hydrogels widely used in drug sustained release, contact lens, tissue engineering scaffold, biological immune regulation, in vitro diagnosis and other fields. However, good biocompatibility makes it easy to be infected ...

Claims

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Application Information

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IPC IPC(8): C08J7/14C08J3/075C08J3/28C08L51/00C08F290/00C08F220/34C08F2/48C08H1/00
CPCC08F2/48C08F290/00C08H1/00C08J3/075C08J3/28C08J7/14C08J2351/00C08L51/00C08L2203/02C08F220/34
Inventor 宁成云何佳鹏谭帼馨周蕾刘燕于鹏
Owner SOUTH CHINA UNIV OF TECH
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