Microecological preparation, preparation method, and applications thereof

A technology of micro-ecological preparations and microbial bacterial liquid, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of farmed animals and human health hazards, drug residues, etc. The effect of sickness

Active Publication Date: 2014-01-08
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in order to maintain the breeding density and prevent the outbreak of diseases, all kinds of antibiotics, such as chloramphenicol, oxytetracycline, furazolidone, etc., are blindly and exc

Method used

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  • Microecological preparation, preparation method, and applications thereof
  • Microecological preparation, preparation method, and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1 A kind of preparation of probiotics

[0036] The cultivation of the bacteria of each component in the probiotics:

[0037] Cultivation of Bacillus subtilis and Bacillus pumilus: The starting strain Bacillus subtilis (Bacillus subtilis1A05680) preservation number CCTCC NO: M2013289 and the strain Bacillus pumilus (Bacillus pumilus1A08151) preservation number CCTCC NO: M2013291 were fermented by slant, liquid seed and liquid Sequential culture, slant and seed medium are conventional broth medium; fermentation medium formula: bean cake powder 3%, starch 1.5%, corn steep liquor 1%, glucose 0.5%, (NH 4 ) 2 SO 4 0.1%,NH 4 Cl0.1%,KH 2 PO 4 0.05%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation culture condition is 37 ℃, ventilated culture 36h, the number of viable bacteria ≥ 10 9 CFU / mL;

[0038] Cultivation of Lactobacillus plantarum and Lactobacillus acidophilus: the starting strain Lactobacillus plantarum (Lactobacillus plantarum1A07806...

Embodiment 2

[0044] Application test 1 of embodiment 2 probiotics of the present invention in prawn culture

[0045] 1. Materials and methods

[0046] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / mL.

[0047] 2. Test prawns: divide the prawns into 2 groups, 250 tails in each group, and set 3 replicates in each group.

[0048] 3. Test water body: The test water is taken from sand-filtered seawater in Baicheng, Xiamen.

[0049] 4. Test site: The test is carried out in a half-ton glass fiber tank. The test cylinders were placed in an open place outdoors, randomly placed under natural conditions.

[0050] 5. Test method: The test period is 45 days. The test adopts the conventional feed feeding of prawns, and the test group adds 1 kg of the test product per ton of feed to ensure that the number of viable bacteria per gram of feed is >10 6 CFU / mL, no test product was added to the control grou...

Embodiment 3

[0060] Application test 2 of embodiment 3 probiotics of the present invention in prawn culture

[0061] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / mL.

[0062] 2. Experimental prawns: use the whole process from throwing seedlings to growing prawns. Set up 3 experimental pools and 7 control pools.

[0063] 3. Test site: a prawn farm in Shentu Town, Zhangpu County.

[0064] 4. Test method: during daily feeding, get the whole pond of the composite bacterial agent of Example 1 and evenly sprinkle it. Use it once every five days, and use 1-2L per mu of aquaculture water body, and double it when the water quality deteriorates. The control group did not use the bacteria agent. Get the composite bacterial agent of embodiment 1 and spray evenly on the feed surface after diluting with appropriate amount of water simultaneously, use 1~2L of every 100kg feed, every day mix material an...

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Abstract

The invention discloses a microecological preparation, a preparation method and applications thereof, and relates to the field of aqua culture. The microbiological preparation comprises at least one component selected from bacillus subtilis and bacillus pumillus, and at least one component selected from lactobacillus plantarum and lactobacillus acidophilus; wherein the bacillus subtilis comprises Bacillus subtilis 1A05680, the bacillus pumillus comprises Bacillus pumilus 1A08151, the lactobacillus plantarum comprises Lactobacillus plantarum 1A07806, and the lactobacillus acidophilus comprises Lactobacillus acidophilus PL7. The microecological preparation can be applied to preparation of preparations, which are used for promoting growth of prawn or grouper, improving immunity and disease resistance of prawn or grouper, preventing and treating diseases, increasing the survival rate, and purifying the culture water.

Description

technical field [0001] The present invention relates to aquaculture, especially a kind of preparation that is applied to prawn or grouper aquaculture, specifically is made of Bacillus subtilis, Bacillus pumilus, Lactobacillus plantarum, Lactobacillus acidophilus in promoting the growth of prawn or grouper. It can be used in fish growth, improving the immunity and disease resistance of prawn or grouper, preventing and treating diseases of prawn or grouper, especially grouper black body disease, improving the survival rate of prawn or grouper, and purifying the water quality of aquaculture. Background technique [0002] Since 2000, with the promotion of high-density farming model, my country's shrimp farming industry has entered a period of rapid development. In 2006, the total amount of shrimp farming in my country was 1.24 million tons, accounting for 39.2% of the global farmed shrimp production, and it has remained the world's first for many years . However, the rapid devel...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61K31/04A61K31/14A61K31/20A23K1/16A23K1/18C02F3/34C12R1/125C12R1/25C12R1/07C12R1/23A61K35/742A61K35/747
Inventor 邵宗泽王丽萍单大鹏黄兆斌
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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