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Method for cultivating large granular nuclear pearls

A technology of large particles and pearls, applied in the field of aquaculture, can solve problems such as high bead spit rate, and achieve the effect of solving high and solving bead spit rate.

Inactive Publication Date: 2010-08-18
SHANGHAI OCEAN UNIV
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Problems solved by technology

[0003] The main technical problem to be solved by the present invention is that in the process of cultivating nucleated pearls by the existing viscera mass, after the pearl nucleus is inserted, the pearl-cultivating organisms (such as triangular sailfish, etc.) generally have the problem of high pearl spit rate, and in view of this problem, provide A method of cultivating nucleated pearls

Method used

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  • Method for cultivating large granular nuclear pearls
  • Method for cultivating large granular nuclear pearls

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Embodiment

[0046] In this example, healthy and vigorous mussels around 1 year old were used as the source of mantle epithelial tissue.

[0047] First, the outer skin tissue of the mantle of the mussel is separated by tearing the membrane method. The membrane tearing method adopted can make the obtained mantle tissue have a single component without inner epidermal tissue or cells, thereby contributing to the acquisition of high-purity mantle outer skin cells.

[0048] Second, in a sterile room, take the outer skin tissue of the mantle in PBS solution (containing 8g / LNaCl, 0.2g / LKCl, 1.44g NaCl, 2 HPO 4 / L and 0.24g / L KH 2 PO 4 , pH 7.4), then wash the tissue once with PBS+200IU / mL double-antibody (penicillin and streptomycin), PBS+1000IU / mL double-antibody, PBS+200IU / mL double-antibody lotion, and finally Rinse again with PBS.

[0049] Third, take the treated tissue and cut it into 2mm 3 Rinse the small piece with PBS twice, then add 0.3% trypsin digestion solution to the tissue pie...

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Abstract

The invention belongs to the technical field of cultivation, and relates to a method for cultivating large granular nuclear pearls. In order to solve the problem that in a traditional process for cultivating nuclear pearls, after pearl cores are inserted, pearl cultivation creature (e.g. freshwater mussel and the like) generally has high pearl production rate, the invention provides a method for cultivating large granular nuclear pearls. The method comprises the following steps that: a. outer mantle epithelial cells are obtained from mussel body; b. the pearl cores are incubated from the obtained outer mantle epithelial cells, and incubated pearl cores are obtained; c. the incubated pearl cores are implanted into pearl cultivating mussel to be cultivated, wherein, step c is divided into the following sub-steps that: (1) a sac for containing the incubated pearl cores is arranged in the pearl cultivating mussel; (2) the incubated pearl cores are stuffed into the sac, and the opening of the sac is sutured by a biological suture; and (3) the pearl cultivating mussel treated in step (2) is cultivated. The method of the invention can smoothly solve the technical problem, so that the diameter of the cultivated pearls is generally above 10mm.

Description

technical field [0001] The invention belongs to the technical field of cultivation, and relates to a method for cultivating nucleated pearls. The method of the invention is used for cultivating incubating pearl nuclei with a diameter of 8-10 mm, so that the cultivated pearls can have a larger diameter (generally can up to 10mm or more), the cultured organisms used are generally freshwater shellfish, such as triangular sail clam. Background technique [0002] my country is rich in fresh water resources, and freshwater shellfish have broad prospects for pearl breeding. At present, the annual output of freshwater pearls in my country exceeds 1,000 tons, but high-quality and high-grade pearls are less than 100 tons. Pearls with a diameter of 8 mm or more cannot meet the requirements of the international market in the short term. The size of pearl particles determines the economic benefits of pearl production to a large extent. It is still a difficult problem in production for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A01K61/56
CPCY02A40/81
Inventor 施志仪李文娟贾亮
Owner SHANGHAI OCEAN UNIV
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