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Rhodotorula mucilaginosa with novel metabolic characteristic and application of the same in biodegradation

A technology of red yeast and metabolic characteristics, applied in the field of bioengineering, can solve the problems of non-degradability and high salinity, and achieve the effect of enhancing processing capacity and broad application potential

Inactive Publication Date: 2008-12-10
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to domesticate and screen out nitrobenzene through scientific methods in view of the characteristics of high salinity and complex components of the actual nitrobenzene industrial wastewater and the problem of the non-degradable by-product picolinic acid in the process of nitrobenzene degradation. High-efficiency degrading bacteria to treat nitrobenzene wastewater with high salinity and complex components, and solve the bottleneck in the degradation process of nitrobenzene

Method used

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  • Rhodotorula mucilaginosa with novel metabolic characteristic and application of the same in biodegradation
  • Rhodotorula mucilaginosa with novel metabolic characteristic and application of the same in biodegradation
  • Rhodotorula mucilaginosa with novel metabolic characteristic and application of the same in biodegradation

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Embodiment 1

[0035] Rhodotorula gum provided by the invention, its screening steps are as follows:

[0036]Samples of aerobic activated sludge were collected from the aeration tank of Dalian Dyestuff Factory, and used as a source of bacteria for domestication and cultivation. Add 0.2% sodium pyrophosphate to 50ml of activated sludge as a deflocculant, vibrate on a shaker for 5-10min to break up the activated sludge flocs, take 5ml of activated sludge, add 95ml of inorganic salt medium to shake In the bottle, add nitrobenzene as the only carbon, nitrogen, and energy source, wherein the concentration of nitrobenzene is 200 mg / L, and carry out shaker cultivation at a temperature of 30° C. and a rotation speed of 180 r / min. Inorganic salt medium composition: Na 2 HPO 4 12H 2 O, 7g / L; KH 2 PO 4 , 1g / L; CaCl 2 2H 2 O, 10mg / L; FeCl 3 , 2mg / L; MgSO 4 ·7H 2 O, 20mg / L. When the bacterial liquid becomes turbid and nitrobenzene cannot be detected, the next transfer is carried out; after con...

Embodiment 2

[0059] Among the present invention, the application of Rhodotorula gum p-nitrobenzene aerobic degradation research, its steps are as follows:

[0060] [1] Add 100ml of inorganic salt medium to a 250ml Erlenmeyer flask, then add 450mg / L of nitrobenzene as the only carbon, nitrogen, and energy source, and sterilize at 121°C;

[0061] [2] Add the liquid culture of Rhodotorula gluei cells prepared in Example 1 into the medium of [1] above, 30°C, 180r / min, aerobic culture, figure 1 The growth and nitrobenzene degradation of Rhodotorula gum.

Embodiment 3

[0063] In the present invention, the application of Rhodotorula gluei to research on the aerobic degradation of picolinic acid, the steps are as follows:

[0064] [1] Add 100ml of inorganic salt medium to a 250ml Erlenmeyer flask, add 3500mg / L picolinic acid as the only carbon, nitrogen, and energy source, and sterilize at 121°C;

[0065] [2] Add the liquid culture of Rhodotorula gluei cells prepared in Example 1 into the medium of [1] above, 30°C, 180r / min, aerobic culture, figure 2 The growth and picolinic acid degradation of Rhodotorula glucosa.

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Abstract

The invention discloses a rhodotorula mucilaginosa with metabolism property and an application thereof in biodegradation, belonging to the bio-engineering technical field. The invention separates and filters a rhodotorula mucilaginosa with the metabolism property. The invention is characterized in that: the rhodotorula mucilaginosa degrades nitrobenzene through partial reduction, and mineralizes a subsidiary product, namely pyridine carboxylic acid produced in degradation process, thereby realizing complete degradation of the nitrobenzene, and solving the bottleneck of partial degrading reduction of the nitrobenzene; the rhodotorula mucilaginosa can effectively degrade the nitrobenzene under the condition of high salinity and coexistence of a plurality of organic substances; the rhodotorula mucilaginosa can grow by taking the pyridine carboxylic acid as the only carbon, nitrogen and energy, and the pyridine carboxylic acid is finally mineralized into harmless carbon dioxide and water; and the rhodotorula mucilaginosa can act as a biological strengthening agent to be applied to the biological treatment of nitrobenzene waste water and pyridine carboxylic acid waste water with high salinity and complex compositions.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a strain of Rhodotorula colloides with new metabolic characteristics and its application in the aerobic biodegradation of nitrobenzene and picolinic acid. Background technique [0002] Nitrobenzene is widely used in the production of medicine, dyes, plastics, explosives, etc. The wide application of nitrobenzene leads to the pollution of the environment by nitrobenzene. Nitrobenzene wastewater can be treated by aerobic biological method. At present, the microbial resources for aerobic degradation of nitrobenzene include bacteria and fungi, and the fungi are mainly white-rot fungi. [0003] The most important use of nitrobenzene in industry is to produce azo dyes, and azo dye wastewater has the characteristics of high salinity and complex composition. High salinity means that the total dissolved solids (sodium chloride) in the wastewater is at least 3.5% (W / V); the complex ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C02F3/34C12R1/85C02F101/38
Inventor 周集体郑春莉王竞吕红曲嫒嫒金若菲项学敏张劲松王静
Owner DALIAN UNIV OF TECH
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