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Producing method of rhodotorula mucilaginosa for holothurian

A production method, the technology of rhodotorula gumi, which is applied in application, animal feed, fungi, etc., can solve the problems of low content of astaxanthin and lysine, slow growth and reproduction of strains, and inability to meet production and application requirements, and achieve The effect of bright color, fast reproduction speed, and easy production and promotion

Inactive Publication Date: 2008-03-26
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

[0006] In recent years, people have attached great importance to the development and utilization of marine resources, especially the application of marine yeast in the breeding and breeding of marine treasures has achieved very good results. The main problems now are: ①The growth and reproduction of the strains in conventional culture methods is slow , Long cultivation time, low bacterial concentration
② The characteristics of the strain are not obvious, the content of astaxanthin and lysine in the nutrition is not high, the strain is impure and easy to degenerate, which cannot meet the needs of production and application

Method used

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Embodiment Construction

[0017] The marine gelatinous rhodotorula for sea cucumbers obtained by the present invention is rich in protein, amino acid, vitamin, unsaturated fatty acid, carotenoid, astaxanthin and other bioactive substances. The chemical name of astaxanthin is 3,3'-dihydroxy-4,4'-diketo-β-carotene, which is a fat-soluble bright red pigment, which has the functions of promoting growth, resisting diseases, and enhancing color, and can promote nutrient digestion and absorption and digestion and utilization of feed.

[0018] The production and fermentation steps of marine gum rhodotorula are as follows:

[0019] 1. Seed parent activation

[0020] Sea cucumbers, such as Rhodotorula mucilaginosa MY96, were transferred to the newly prepared slant of Kjeldahl flasks containing Rhodotorula mucilaginosa medium, activated and cultured for 72-96 hours at a temperature of 25°C to activate and mature The bacteria in the final Kelvin flask are called seeds. The composition of the slant medium for se...

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Abstract

The present invention relates to marine microbe applying technology and sea cucumber cultivating technology, and is especially process of producing marine rhodotorula mucilaginosa for sea cucumber. The conventional marine rhodotorula mucilaginosa is cultured in aged sea water with glucose in 1.0-3.8 wt% and EDTA-Fe in 1-3 ml / l at 15-25 deg.c, and the production process includes two stages of fermentation, centrifugal concentration and other steps. The production is simple, short in production period and low in cost, and the marine rhodotorula mucilaginosa product has high quality. The produced marine rhodotorula mucilaginosa is applied in raising sea cucumber larva to raise the metamorphosis rate and survival rate of sea cucumber larva, promote the growth of the larva and improve the ecological environment in the culture pond.

Description

technical field [0001] The invention relates to marine microorganism application technology and sea cucumber cultivation, in particular to a production method of marine red yeast for sea cucumbers. Background technique [0002] Marine red yeast (Rhodotorula glutinis Harrison) belongs to the kingdom of fungi, the phylum of fungi, the subphylum of myxomycota, and the family of cryptococcomyces, and its mode of reproduction is asexual budding. Its ecological distribution is wide, mainly distributed in seawater, sea mud, and sea treasure breeding ponds. Its carbon source and nitrogen source are widely used, and glucose, maltose, sucrose, galactose, arabinose, mannitol, succinic acid, citric acid, xylose, sorbitol, fructose, ethanol and glycerin can be used; rhamnose, starch cannot be used , cellobiose, trehalose, ribitol, inositol, erythritol, melibiose. And it has the characteristics of fast growth and reproduction, easy cultivation, rich nutrition of bacteria and metabolites...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23K1/18A23K50/80
Inventor 薛德林胡江春王国安王向阳马成新刘丽王书锦
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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