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Rhodotorula mucilaginosa mutant strain and application thereof

A technology of Rhodotorula colloides and mutant strains, applied in the field of bioengineering, can solve the problems of being unsuitable for direct development and application and commercial production, affecting the production of carotenoids, and low carotenoid content, so as to achieve convenient and quick addition process and good promotion Application prospect, effect of reducing diarrhea rate

Active Publication Date: 2018-02-16
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, the content of carotenoids in Rhodotorula strains directly screened from nature is relatively low, and the biomass of bacteria is relatively small, which directly affects the production of carotenoids, and is not suitable for direct development, application and commercial production.

Method used

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  • Rhodotorula mucilaginosa mutant strain and application thereof
  • Rhodotorula mucilaginosa mutant strain and application thereof
  • Rhodotorula mucilaginosa mutant strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1 Mutagenesis screening of high carotenoid-producing Rhodotorula mutagenic strains

[0040] 1. Determination of lethality and mutagenesis conditions of compound mutagenesis of starting strains

[0041] (1) Bacterial suspension preparation: starting strain Rhodotorula rubrum ZT (this bacterial strain is obtained from the mangrove leaves of Leizhou Peninsula coastal waters isolated and bred by the inventor of the present application, Xu Chunhou in 2014) is inoculated on solid medium ( 2% peptone, 2% yeast extract, 1% beef extract, 2% glucose, 2% sodium chloride, 2% agar, natural pH) plate, incubate at 28°C for 48h, scrape off the bacterial lawn with sterile saline, 4 Filter through layers of gauze, the filtrate is the bacterial suspension of the starting strain, and the number of viable bacteria is adjusted to 1.3×10 7 CFU / mL followed by subsequent mutagenesis treatment.

[0042] (2) Compound mutagenesis method: take 9 mL of the bacterial suspension of the startin...

Embodiment 2

[0050] Example 2 Phenotypic identification and gene sequence determination of mutant strains

[0051] 1. Identification of strain phenotype

[0052] (1) Morphological characteristics of colony: The colony of mutant strain ZTHY2 is dark red, round, raised, neat edge, opaque, moist and sticky.

[0053] (2) Morphological characteristics of cells: The cell shape of mutant strain ZTHY2 is oval.

[0054] (3) Physiological and biochemical characteristics: The urease test and diazo blue B test of the mutant strain ZTHY2 were positive, and it did not ferment glucose, lactose, galactose, maltose, sucrose, melibiose, raffinose, melanotriose and inulin , grown at 25°C, 30°C, and 35°C.

[0055] 2. Determination of gene sequence

[0056] (1) The gene sequence of the 26S rDNA D1 / D2 region of the mutant strain ZTHY2 is shown in SEQ ID NO.1.

[0057] To sum up, after the identification and analysis of the morphological characteristics, physiological and biochemical characteristics and the ...

Embodiment 3

[0058] The preparation of embodiment 3 animal growth promoter

[0059] An animal growth promoter is prepared by using a mutant strain of Rhododendron, and its preparation method is as follows:

[0060] (1) Take Rhodotorula rubrum ZTHY2, and after activation, ferment and culture at 31°C and 150r / min for 96h to obtain an animal growth promoter;

[0061] Among them, the medium components of the activation culture are: peptone 2%, yeast extract 2%, beef extract 1%, glucose 2%, sodium chloride 2%, the above are all mass ratios, and the pH value is natural;

[0062] The medium components of the fermentation culture are: soybean peptone 2%, yeast extract 2%, beef extract 1%, glucose 2%, sodium chloride 2%, manganese sulfate 0.075%, the above are all mass ratios, and the pH value is natural;

[0063] (2) It was determined that the viable count of Rhododendron in the animal growth promoter was 3.6×10 10 CFU / mL, carotenoid yield was 52mg / L.

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Abstract

The invention discloses a rhodotorula mucilaginosa mutant strain and application thereof. The rhodotorula mucilaginosa mutant strain disclosed by the invention is Rhodotorula mucilaginosa ZTHY2 and ispreserved in China Center for Type Culture Collection on May 13th, 2015 with the preservation number of CCTCC NO:M 2015296. The mutant strain is obtained by adopting a compound mutation method, yieldof carotenoid can reach 39mg / L within 72 hours and is 3.6 times of an original strain, the yield can reach 52mg / L after fermentation is carried out for 96 hours, and the rhodotorula mucilaginosa mutant strain has relatively high application value in preparation of an animal growth promoter or an animal feed additive, especially in aspects of improving production performance of sanhuang chicken and weaning piglets, improving quality of eggs and lowering content of cholesterol in egg yolk. Besides, the mutant strain disclosed by the invention is stable in performance, easy to culture, high in breeding speed and high in mycelium biomass and is obviously better than a Rhodotorula strain directly screened from nature.

Description

technical field [0001] The present invention relates to the technical field of bioengineering. More specifically, it relates to a mutant strain of Rhodotorulatum and its application. Background technique [0002] Marine red yeast refers to single-celled eukaryotic microorganisms that exist in the marine environment and marine organisms. In different marine environments such as Antarctica, the Pacific Ocean, the southwest coast of India, deep-sea volcanoes, and some polluted sea areas, red yeast is the dominant flora ; and Rhodotorula japonica is the dominant population in the coastal waters of Leizhou Peninsula (South China Sea). Marine red yeast is rich in protein, unsaturated fatty acids, carotenoids, digestive enzymes, vitamins, etc., and has high nutritional value and activity. It can be used as food additives, functional biological feeds, antioxidants, colorants, etc. The main species for the production of carotenoids. [0003] Carotenoid is an orange-yellow fat-solu...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23K10/18C12P23/00C12R1/645
CPCA23K10/18C12N1/16C12P23/00C12N1/145C12R2001/645
Inventor 徐春厚刘颖谢为天邝雪梅
Owner 山东宝来利来生物工程股份有限公司
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