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Bacillus amyloliquefaciens and application thereof

A technology for dissolving amyloid spores and bacilli, applied in the field of microorganisms, can solve the problems of complex production process, increasing the production cost of biological deodorants, and limiting the application scope of biological deodorants.

Inactive Publication Date: 2017-05-31
JIANGXI SHUNQUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the biological deodorant needs to be prepared by separately fermenting five different microorganisms and then mixing them together, the production process is complicated, which increases the production cost of the biological deodorant
The invention patent with the application number 01129774.3 discloses a microbial deodorant and its preparation method and application. The biological deodorant is only prepared by the mixed fermentation of two yeasts, Saccharomyces utilis and Saccharomyces ellipsoides in the genus Saccharomyces. Therefore limit the scope of application of this biological deodorant

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The strain origin, strain isolation and gene sequence determination of Bacillus amyloliquefaciens

[0035] Source of the strain: It was isolated and selected from the soil of the domestic waste sanitary landfill in Fuzhou City, Jiangxi Province. The strain number is SQ-001. On August 30, it was deposited in the General Microorganism Center (CGMCC) of China Committee for the Collection of Microorganisms in Beijing. The strain preservation number of this strain of Bacillus amyloliquefaciens is CGMCC No.12917. Described bacterial strain SQ-001, thalline is rod-shaped, Gram's staining is positive, forms spore, does not expand; Conventional physiological and biochemical experiments show that it is an aerobic bacterium, contact enzyme, oxidase, VP test is positive, in 7 % NaCl growth, pH5.7 growth, hydrolysis of starch, reduction of nitrate to nitrite.

[0036] Described bacterial strain SQ-001 obtains by following specific separation steps:

[0037] A sample collection: co...

Embodiment 2

[0044] Preparation of compound microbial deodorant

[0045] (1) Seed preparation

[0046] Seed medium: glucose 2.5g / 100mL, yeast extract 0.5g / 100mL, corn steep liquor 2.5g / 100mL, water, adjust pH to 6.5. Inoculate Bacillus amyloliquefaciens CGMCC No.12917, Rhodotorula gum CGMCC No.2.4008, Enterococcus faecalis CGMCC No.1.2024 and Acetobacter pasteurianus CGMCC No.1.2830 into the above-mentioned seed medium. , Cultivate at 32°C for 24 hours to obtain seeds suitable for inoculation.

[0047] (2) Mixed bacteria liquid fermentation culture

[0048] Fermentation medium: glucose 2.5g / 100mL, yeast extract 0.3g / 100mL, corn steep liquor, 4g / 100mL, potassium dihydrogen phosphate 0.03g / 100mL, magnesium sulfate 0.03g / 100mL. The acetic acid bacteria seeds obtained in (1) were inoculated in the fermentation medium according to 6% of the volume of the fermentation broth, and after being cultivated at 32° C. for 12 h at a rotating speed of 150 rpm on a rotary shaker, they were then inserte...

Embodiment 3

[0051] Preparation of compound microbial deodorant

[0052] (1) Seed preparation

[0053] Seed medium: glucose 5g / 100mL, yeast extract 1g / 100mL, corn steep liquor 3g / 100mL, water, adjust pH to 6.0. Inoculate Bacillus amyloliquefaciens CGMCC No.12917, Rhodotorula gum CGMCC No.2.4008, Enterococcus faecalis CGMCC No.1.2024 and Acetobacter pasteurianus CGMCC No.1.2830 into the above seed medium, and in a rotary shaker at 180rpm , Cultivate at 30°C for 24 hours to obtain seeds suitable for inoculation.

[0054] (2) Mixed bacteria liquid fermentation culture

[0055] Fermentation medium: glucose 3g / 100mL, yeast extract 0.5g / 100mL, soybean powder 4g / 100mL, potassium dihydrogen phosphate 0.05g / 100mL, magnesium sulfate 0.04g / 100mL. The Acetobacter pasteurian seeds obtained in (1) were inoculated in the fermentation medium according to 5% of the volume of the fermentation broth, and were cultured at 30° C. for 24 h under the rotating speed of 170 rpm in a rotary shaker, and then inoc...

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Abstract

The invention relates to the technical field of microorganisms and particularly relates to a bacillus amyloliquefaciens. The bacillus amyloliquefaciens is separated and bred from soil of Jiangxi Fuzhou domestic refuse landfill site; the strain number is SQ-001; the Latin name is bacillus amyloliquefaciens; the strain SQ-001 is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 30, 2016; the preservation address is Building 3, Yard 1, West Beichen Street, Chaoyang District, Beijing; the postcode is 100101; the strain preservation number is CGMCC No.12917. The bacillus amyloliquefaciens strain SQ-001, rhodotorula mucilaginosa CGMCC No. 2.4008, enterococcus faecalis CGMCC No. 1.2024 and acetobacter pasteurianus CGMCC No. 1.2830 are prepared into a composite microbial deodorant through processes of purebred amplification culture and mixed fermentation; the composite microbial deodorant is excellent in deodorization effect and wide in application range.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Bacillus amyloliquefaciens and its application. Background technique [0002] In the biosphere, the living range of microorganisms is the widest and most three-dimensional, and microorganisms are an extremely large team in the biological world. Numerous facts have vividly proved that since human beings have known microorganisms and gradually mastered the laws of their activities, it is possible to make microorganisms that were originally unprofitable become beneficial, those with small profits to become big, and those that are harmful to become small, harmless or even beneficial, thus Greatly promote the progress of mankind. my country is a country rich in rare microbial resources due to its vast land, complex terrain, cold, warm and hot zones, and diverse ecological environments. Due to historical reasons, the research and application of soil micr...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84C12N1/16C12R1/07C12R1/645C12R1/46C12R1/02
CPCB01D53/84B01D2251/95C12N1/16C12N1/20C12N1/205C12R2001/07Y02P20/59Y02A50/20
Inventor 张海华李明林陈文
Owner JIANGXI SHUNQUAN BIOTECH CO LTD
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