Bacillus amyloliquefaciens and application thereof
A technology for dissolving amyloid spores and bacilli, applied in the field of microorganisms, can solve the problems of complex production process, increasing the production cost of biological deodorants, and limiting the application scope of biological deodorants.
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[0033] Example 1
[0034] Strain source, strain isolation, gene sequence determination of Bacillus amyloliquefaciens
[0035] Strain source: isolated and selected from the soil of the domestic waste sanitary landfill in Fuzhou, Jiangxi. The strain number is SQ-001. It was identified as Bacillus amgloliquefaciens by the China Microbial Culture Collection, and was established in 2016 On August 30, it was deposited in the General Microbiology Center (CGMCC) of the China Microbial Culture Collection Management Committee in Beijing. The deposit number of this strain of Bacillus amyloliquefaciens is CGMCC No. 12917. Said strain SQ-001 has rod-shaped cells, positive Gram staining, spore formation, and no swelling; routine physiological and biochemical experiments show that it is an aerobic bacteria, exposed to enzymes, oxidases, and VP test is positive. % NaCl growth, PH5.7 growth, hydrolyze starch, reduce nitrate to nitrite.
[0036] The strain SQ-001 was obtained through the following s...
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[0043] Example 2
[0044] Preparation of compound microbial deodorant
[0045] (1) Seed preparation
[0046] Seed culture medium: glucose 2.5g / 100mL, yeast extract 0.5g / 100mL, corn steep liquor 2.5g / 100mL, water, adjust pH to 6.5. Bacillus amyloliquefaciens CGMCC No. 12917, Rhodotorula glutinis CGMCC No. 2.4008, Enterococcus faecalis CGMCC No. 1.2024 and Acetobacter pasteurian CGMCC No. 1.2830 were inoculated into the above seed culture medium, and the rotation speed of the rotary shaker was 150 rpm , Cultivate at 32℃ for 24h to get seeds suitable for inoculation.
[0047] (2) Mixed bacteria liquid fermentation culture
[0048] Fermentation medium: glucose 2.5g / 100mL, yeast extract 0.3g / 100mL, corn steep liquor, 4g / 100mL, potassium dihydrogen phosphate 0.03g / 100mL, magnesium sulfate 0.03g / 100mL. The acetic acid bacteria seeds obtained in (1) were inoculated into the fermentation medium according to 6% of the volume of the fermentation broth. After incubating at 32°C for 12 hours on a...
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[0050] Example 3
[0051] Preparation of compound microbial deodorant
[0052] (1) Seed preparation
[0053] Seed culture medium: glucose 5g / 100mL, yeast extract 1g / 100mL, corn steep liquor 3g / 100mL, water, adjust the pH to 6.0. Bacillus amyloliquefaciens CGMCC No. 12917, Rhodotorula glutinosa CGMCC No. 2.4008, Enterococcus faecalis CGMCC No. 1.2024 and Acetobacter pasteurium CGMCC No. 1.2830 were inoculated into the above-mentioned seed culture medium at 180 rpm in a rotary shaker , Cultivate for 24h at 30°C to get seeds suitable for inoculation.
[0054] (2) Mixed bacteria liquid fermentation culture
[0055] Fermentation medium: glucose 3g / 100mL, yeast extract 0.5g / 100mL, soybean powder 4g / 100mL, potassium dihydrogen phosphate 0.05g / 100mL, magnesium sulfate 0.04g / 100mL. The Acetobacter pasteurian seeds obtained in (1) were inoculated into the fermentation medium according to 5% of the volume of the fermentation broth, and incubated at 30°C for 24 hours on a rotary shaker at 170 rp...
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