Compound microorganism deodorant and preparation method thereof

A technology for compound microorganisms and deodorants, which is applied in the field of compound microorganism deodorants and their preparation, can solve the problems of complex production process, limit the application scope of biological deodorants, increase the production cost of biological deodorants, etc., and reach the application scope. Wide and good deodorizing effect

Active Publication Date: 2017-05-31
JIANGXI SHUNQUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the biological deodorant needs to be prepared by separately fermenting five different microorganisms and then mixing them together, the production process is complicated, which increases the production cost of the biological deodorant
The invention patent with the application number 01129774.3 discloses a microbial deodorant and its preparation method and application. The biological deodorant is only prepared by the mixed fermentation of two yeasts, Saccharomyces utilis and Saccharomyces ellipsoides in the genus Saccharomyces. Therefore limit the scope of application of this biological deodorant

Method used

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  • Compound microorganism deodorant and preparation method thereof
  • Compound microorganism deodorant and preparation method thereof
  • Compound microorganism deodorant and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0033] The strain origin, strain isolation and gene sequence determination of Bacillus amyloliquefaciens:

[0034] Source of the strain: It was isolated and selected from the soil of the domestic waste landfill in Fuzhou City, Jiangxi Province. The strain number is SQ-001. On August 30, it was deposited in the General Microorganism Center (CGMCC) of China Committee for the Collection of Microorganisms in Beijing. The strain preservation number of this strain of Bacillus amyloliquefaciens is CGMCC No.12917. The bacterial strain SQ-001 described in the bacterial strain SQ-001 has a rod shape, Gram staining is positive, and spores are formed without swelling; routine physiological and biochemical experiments show that it is an aerobic bacterium, and the contact enzyme, oxidase, VP test shows Positive, grow in 7% NaCl, grow at PH5.7, hydrolyze starch, reduce nitrate to nitrite.

[0035] Described bacterial strain SQ-001 obtains by following specific separation steps:

[0036] A ...

Embodiment 2

[0043] Preparation of compound microbial deodorant

[0044] (1) Seed preparation

[0045] Seed medium: glucose 2.5g / 100mL, yeast extract 0.5g / 100mL, corn steep liquor 2.5g / 100mL, water, adjust pH to 6.5. Inoculate Bacillus amyloliquefaciens CGMCC No.12917, Rhodotorula gum CGMCC No.2.4008, Enterococcus faecalis CGMCC No.1.2024 and Acetobacter pasteurianus CGMCC No.1.2830 into the above-mentioned seed medium. , Cultivate at 32°C for 24 hours to obtain seeds suitable for inoculation.

[0046] (2) Mixed bacteria liquid fermentation culture

[0047] Fermentation medium: glucose 2.5g / 100mL, yeast extract 0.3g / 100mL, corn steep liquor, 4g / 100mL, potassium dihydrogen phosphate 0.03g / 100mL, magnesium sulfate 0.03g / 100mL. The acetic acid bacteria seeds obtained in (1) were inoculated in the fermentation medium according to 6% of the volume of the fermentation broth, and after being cultivated at 32° C. for 12 h at a rotating speed of 150 rpm on a rotary shaker, they were then inserte...

Embodiment 3

[0051] Preparation of compound microbial deodorant

[0052] (1) Seed preparation

[0053] Seed medium: glucose 5g / 100mL, yeast extract 1g / 100mL, corn steep liquor 3g / 100mL, water, adjust pH to 6.0. Inoculate Bacillus amyloliquefaciens CGMCC No.12917, Rhodotorula gum CGMCC No.2.4008, Enterococcus faecalis CGMCC No.1.2024 and Acetobacter pasteurianus CGMCC No.1.2830 into the above seed medium, and in a rotary shaker at 180rpm , Cultivate at 30°C for 24 hours to obtain seeds suitable for inoculation.

[0054] (2) Mixed bacteria liquid fermentation culture

[0055]Fermentation medium: glucose 3g / 100mL, yeast extract 0.5g / 100mL, soybean powder 4g / 100mL, potassium dihydrogen phosphate 0.05g / 100mL, magnesium sulfate 0.04g / 100mL. The Acetobacter pasteurian seeds obtained in (1) were inoculated in the fermentation medium according to 5% of the volume of the fermentation broth, and were cultured at 30° C. for 24 h under the rotating speed of 170 rpm in a rotary shaker, and then inocu...

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Abstract

The invention relates to the technical field of biological deodorization, and specifically discloses a compound microorganism deodorant and a preparation method thereof. The compound microorganism deodorant is microorganism fermentation liquor, and the method comprises the following steps: taking bacillus amyloliquefaciens CGMCC No.12917, Rhodotorula mucilaginosa CGMSS No.2.4008, enterococcus faecalis CGMCC No.1.2024 and acetobacter pasteurianus CGMCC No.1.2830 as the strain, performing pure breed expanding cultivation and mixed bacteria liquid fermentation culture, and finally obtaining the microorganism deodorant of viable bacteria at dormant state, wherein the total bacteria count of the microorganism strain is not less than 3.0*1010cfu / mL. The compound microorganism deodorant disclosed by the invention is good in deodorization effect and wide in application range, the provided mixed bacteria fermentation process is simple in preparation method, the production period is shortened, the utilization ratio of the production equipment is improved, the production cost is lowered, and the large-scale popularization and application is realized.

Description

technical field [0001] The invention belongs to the technical field of biological deodorization, and in particular relates to a compound microbial deodorant and a preparation method thereof. Background technique [0002] Stench refers to all gaseous substances that stimulate the olfactory organs to cause people's unpleasantness and damage the living environment. It is a form of air pollution. Stench has become one of the seven typical public hazards in the world today and has attracted widespread attention from countries and people all over the world. The main types of malodorous gases are H 2 S, SO 2 , mercaptans, ammonia, amines, halogenated hydrocarbons, etc. The main sources of malodorous gas are domestic sources and industrial sources. Life sources refer to the stench produced in daily life, such as household toilets, public toilets, sewage treatment plants, garbage transfer stations, etc.; industrial sources refer to the stench produced in industrial production, su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16B01D53/84C12R1/07C12R1/645C12R1/02
CPCB01D53/84C12N1/16C12N1/20C12N1/205C12R2001/07Y02A50/20
Inventor 张保国张海华李明林
Owner JIANGXI SHUNQUAN BIOTECH CO LTD
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