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Rhodotorula mucilaginosa strain, peanut cadmium-reducing agent and application thereof

A technology of yeast strain and Rhododendron glabrata, applied in the application, biocide, plant growth regulator and other directions, can solve the problems of poor stress resistance, low yield, and restrict the large-scale promotion of varieties, so as to improve the emergence rate and improve the resistance of seeds. sexual effect

Active Publication Date: 2018-05-15
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, selecting special crop varieties, screening or breeding low-accumulation varieties of cadmium is a way to reduce the accumulation of cadmium in edible parts of plants, but due to the particularity of plant varieties, there may be certain defects in their biological characteristics, such as Low, poor stress resistance, etc., which limit the large-scale promotion of varieties

Method used

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  • Rhodotorula mucilaginosa strain, peanut cadmium-reducing agent and application thereof
  • Rhodotorula mucilaginosa strain, peanut cadmium-reducing agent and application thereof
  • Rhodotorula mucilaginosa strain, peanut cadmium-reducing agent and application thereof

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preparation example Construction

[0031] The preparation method of described rhodotorula gum fermented liquid, comprises the following steps:

[0032] The Rhodotorula gelatin strain described in the above scheme is activated and expanded to obtain the fermented seed liquid of the Rhodotorula gelatin strain, and the fermented seed liquid of the Rhodotorula gelatin strain is inoculated into the wort liquid culture medium at an inoculum size of 2.5% to 5%. , under the conditions of 25-30°C and a rotating speed of 150-200r / min, liquid fermentation was carried out for 72h-96h to obtain the fermented liquid of Rhodotorula japonicus;

[0033] The preparation method of described rhodotorula gum solid preparation, comprises the following steps:

[0034] a. The rhodotorula japonicus strain described in the above scheme is activated and expanded to obtain the fermented seed liquid of the rhodotorula japonicus strain, and the fermented seed liquid of the rhodotorula japonicus strain is inoculated into the wheat bran mediu...

Embodiment 1

[0060] Soil for test:

[0061] Collected from polluted soil (sandy loam) in Pingdu City, Qingdao City, its basic physical and chemical properties are: pH 6.4 (soil-water ratio 1:2.5), organic matter 14.8g / kg, cation exchange capacity 15.9cmol / kg, total cadmium content 3.05mg / kg. The soil samples were naturally air-dried, ground and passed through a 2mm (10 mesh) sieve, and used for pot plant simulation experiments. (The depth of cultivated soil in the field is about 20cm, according to the density of the soil (2.65g / m 3 ) and the depth of cultivated soil, the soil weight per mu of field is about 353t. According to this standard, the amount of cadmium reducing agent added in the pot test was converted. All embodiments in the present invention are converted according to this method)

[0062] Test plants

[0063] Peanuts (Fenghua No. 3) were purchased from Shandong Academy of Agricultural Sciences.

[0064] Pot experiment implementation

[0065] Put 21.0kg of air-dried soi...

Embodiment 2

[0068] Soil for test:

[0069] Collected from polluted soil (sandy loam) in Pingdu City, Qingdao City, its basic physical and chemical properties are: pH 6.4 (soil-water ratio 1:2.5), organic matter 14.8g / kg, cation exchange capacity 15.9cmol / kg, total cadmium content 3.05mg / kg. The soil samples were naturally air-dried, ground and passed through a 2mm (10 mesh) sieve, and used for pot plant simulation experiments.

[0070] Test plants

[0071] Peanuts (Fenghua No. 3) were purchased from Shandong Academy of Agricultural Sciences.

[0072] Pot experiment implementation

[0073] Put 21.0kg of air-dried soil samples through a 2mm sieve into each pot, add distilled water until the field water holding capacity of the soil is 16%, keep the soil water content balanced for 1 week and start sowing.

[0074] Select plump seeds, soak the seeds with a volume concentration of 1% sodium hypochlorite solution for 10 minutes, take them out, wash the seeds with deionized water for 5 to 7 ...

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Abstract

The invention provides a rhodotorula mucilaginosa strain, a peanut cadmium-reducing agent and application thereof, which belong to the technical field of crop safety production. According to the peanut cadmium-reducing agent comprising a rhodotorula mucilaginosa fermentation liquor or a rhodotorula mucilaginosa solid preparation provided by the invention, the content of cadmium in peanut seeds canbe reduced. Meanwhile, the P content in a leaf, the yield of the peanut seeds and the biomass of a peanut root system can be also improved. The cadmium-reducing agent provided by the invention also comprises a sulfosalicylic acid solution with the mass concentration being 1 to 2g / L, so that the removal rate of the cadmium in the peanut seeds can be further improved. Compared with peanuts withoutbeing applied with the cadmium-reducing agent, according to the peanuts cultivated by adopting the cadmium-reducing agent provided by the invention, the cadmium content in the peanut seeds can be reduced by 40.63 percent to 44.78 percent, meanwhile, the phosphorus content is improved by 18.18 percent to 21.43 percent, the dry weight of the peanut root system is improved by 31.13 percent to 37.9 percent, and the yield of the peanut seeds is improved by 20.85 percent to 22.2 percent.

Description

technical field [0001] The invention belongs to the technical field of crop safety production, and in particular relates to a rhodotorula japonicus strain, a peanut cadmium reducing agent and applications thereof. Background technique [0002] With the development of industry and the modernization of agricultural production, heavy metal pollution in farmland is becoming more and more serious, and the polluted area is expanding year by year. Heavy metals have attracted extensive attention due to their characteristics such as not easy to degrade and easy to enrich. Due to its high toxicity and high mobility, cadmium (Cd) is considered to be the most important environmental pollutant. Excessive cadmium in the human body can lead to a series of diseases such as bone pain, kidney disease, and liver damage. After entering the soil, cadmium is preferentially combined with carbonate to form insoluble cadmium carbonate, which has greater mobility and plant availability, so it is mor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A01N63/04A01P21/00A01G7/06C12R1/645
CPCA01G7/06A01N63/30C12N1/16C12N1/145C12R2001/645
Inventor 刘君宋宁宁李绍静王芳丽王凯荣
Owner QINGDAO AGRI UNIV
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