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Rhodotorula mucilaginosa capable of degrading urethane and its application in liquor products and food

A technology of urethane and Rhodotorula gelatin, applied in the field of bioengineering, to achieve the effect of broad application potential

Active Publication Date: 2012-06-13
HUZHOU LAOHENGHE BREWING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of these can fundamentally solve the pollution problem of EC in brewing wine. Once EC is formed, it is extremely stable and cannot be degraded by urease. Therefore, the current methods are helpless for the formed EC.

Method used

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  • Rhodotorula mucilaginosa capable of degrading urethane and its application in liquor products and food
  • Rhodotorula mucilaginosa capable of degrading urethane and its application in liquor products and food
  • Rhodotorula mucilaginosa capable of degrading urethane and its application in liquor products and food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Glue Rhodotorula capable of degrading EC ( Rhodotorula mucilaginosa ) Screening of CGMCC No: 5081 strain

[0024] The bacterial strains involved in the present invention are derived from the production of Maotai-flavored liquor such as 'Kouzijiao', 'Langjiu', 'Jinshiyuan', 'Maotai' and other rice wines, wheat koji from healthy mice Feces and feces of mice gavaged with EC (10 mg / kg / d). Shake a certain amount of the sample to be separated in sterile normal saline, inoculate an appropriate amount in 50mL enrichment medium with EC as the only carbon source, and add inorganic salts, shake culture at 30°C, 150rpm on a shaker, when the bacteria When the solution became turbid, the next transfer was carried out, and a stable bacterial solution that could use EC as the only carbon source was obtained through continuous acclimatization. Dilution coating was carried out on the solid medium in which EC was the only carbon source and an indicator was added, and a single colony cap...

Embodiment 2

[0027] Physiological and biochemical properties of the strain

[0028] Strain CGMCC No: 5081 does not ferment sugar, can assimilate glucose, sucrose, maltose, cellobiose, galactose, D-xylose, D-ribose, succinic acid, citric acid and other carbon sources, but cannot assimilate inositol, It can assimilate inorganic nitrogen sources such as ammonium sulfate, ammonium nitrate, and ammonium chloride, but cannot assimilate urea. The growth temperature range is 4-37 ℃, and the optimum temperature is 25-33 ℃; the growth pH range is 2.0-12.0, preferably 4.0-8.0; it can be grown in the environment containing 400g / L glucose, 2.0 mol / L KCl and 6% ethanol respectively. The environment is growing.

Embodiment 3

[0030] Glue Rhodotorula ( Rhodotorula mucilaginosa ) CGMCC No: 5081 strain aerobic degradation EC function

[0031] (1) Add 50mL seed medium to a 250mL Erlenmeyer flask. The medium components are: yeast extract (1%), peptone (1%), glucose (2%), potassium dihydrogen phosphate (0.25%), dihydrogen phosphate Ammonium (1%), Ammonium Sulfate (0.4%), Sodium Chloride (0.2%), Magnesium Sulfate Heptahydrate (0.05%), Zinc Sulfate Heptahydrate (0.02%), Ferrous Sulfate Heptahydrate (0.005%), Tetra Manganese sulfate hydrate (0.015%). In addition, ethyl carbamate or its analogues such as methyl carbamate, propyl carbamate, or amide compounds such as formamide, acetamide, butanamide, lactamide, N-methylacetamide, penicillin sodium, etc. are added naturally. pH: Inoculate the CGMCC No: 5081 strain obtained in Example 1 above, at 30°C, 150rpm, and carry out aerobic culture for 36-60 hours;

[0032] (2) Take the cultured bacterial solution in the above (1) and transfer the inoculation amount ...

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Abstract

The invention relates to Rhodotorula mucilaginosa capable of degrading urethane and its application in liquor products and food, which belongs to the biology engineering technical field. The Ethylcarbamate is called EC for short and Urethane. The invention provides a yeast strain capable of degrading urethane, the classification name is Rhodotorula mucilaginosa which is preserved at the common microorganisms center of the CCCCM with the preservation number of 5081. The strain is from Daqu brewed by Chinese liquor, Chinese rice wine wheat Qu, healthy mice manure and gavage EC mice manure, the strain grows by taking EC as sole carbon source, and the EC is finally decomposed to NH4<+>, C2H5OH and CO2 which are no toxic. The Rhodotorula mucilaginosa can be used for brewing the wine, liquor, prepared wine and other food, and is capable of reducing the EC content and food security.

Description

technical field [0001] The invention relates to a rhodotorula japonicus capable of degrading ethyl carbamate and a method for improving food safety by enzymatically degrading ethyl carbamate in wine and food, belonging to the technical field of bioengineering. Background technique [0002] Ethyl carbamate (EC for short, also known as Urethane) is a natural component of tobacco leaves and cigarettes, as well as fermented food (such as bread, yogurt, cheese, etc.) and alcoholic beverages (such as wine, brandy, whiskey, fruit wine, Accompanying products of Chinese rice wine and Japanese sake, etc.). It can cause lung tumors, lymphoma, liver cancer, skin cancer, etc. Since 2002, EC has become one of the key substances monitored by the World Health Organization. Human dietary intake of ethyl carbamate mainly comes from fermented foods and beverages, of which alcoholic beverages are known to be the main source of ethyl carbamate. According to the conclusions of the MOE (Maximum...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/02C12H1/15A23L1/015C12R1/645A23L5/20
Inventor 徐岩吴群
Owner HUZHOU LAOHENGHE BREWING
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