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Method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases

A technology for fruit postharvest disease, Rhodotorula colloides, applied in the field of fruit postharvest biological control

Inactive Publication Date: 2011-12-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant reports at home and abroad on whether phytic acid culture can affect the effectiveness of postharvest antagonistic yeast biological control

Method used

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  • Method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases
  • Method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases
  • Method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Phytic acid enhances the inhibitory effect of Rhodotorula gluei on fruit diseases

[0031] 1. Test plan

[0032] After the fruit has been processed, wounds of uniform size and depth are formed on the equatorial part of the surface of each fruit with a sterile punch. Among them, the surface wound of strawberry is 3mm (diameter)×3mm (depth), the surface wound of peach fruit is 5mm (diameter)×3mm (depth), and the surface wound of apple is 5mm (diameter)×3mm (depth). Add 30 μl (1) 1×10 equal amount to each wound 8 each / ml yeast suspension diluted with sterile saline; (2) 1×10 8 pcs / ml yeast suspension diluted with phytic acid (containing 0.1%-0.5% phytic acid); (3) Sterile water (as a control). After 3 hours, an equal amount of 30 μl of pathogenic bacteria spore suspension was added to each wound. Among them, add 1×10 strawberries respectively 5 Botrytis cinerea ( Botrytis cinerea ) spore suspension and 1 x 10 4 Rhizopus glucoides per ml ( Rhizopus...

Embodiment 2

[0045] Example 2: Effects of Phytic Acid Enhanced Rhodotorula glucosa on Fruit Natural Rot and Storage Quality

[0046] 1. Test plan

[0047] The test fruits were directly soaked for 30s with the following treatments after picking: (1) Rhodotorula gum suspension cultured in NYDB medium; (2) Yeast suspension diluted with phytic acid solution; (3) Sterile water (as a control). Then put the fruit in a plastic basket, air-dry and seal it with plastic wrap. Store strawberries in a 4°C freezer, transfer them to a 20°C incubator for storage after 20 days, observe the fruit rot after 5 days, and calculate the rot rate; store peaches in a 4°C freezer, transfer them to a 25°C incubator for storage after 30 days, and observe the fruit rot after 7 days The apples were stored in a 4°C freezer, and then transferred to a 25°C incubator for storage after 30 days. After 10 days, the fruit rot was observed, the rot rate was calculated, and the storage quality was analyzed. Each treatment wa...

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Abstract

The invention discloses a method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases. The method comprises the following steps of: activating the rhodotorula mucilaginosa, inoculating the activated rhodotorula mucilaginosa to an NYDB culture medium, culturing, and performing centrifugation to obtain bacteria; diluting the bacteria by using phytic acid solution to prepare 1*10<8> bacteria per milliliter bacteria suspension; putting fruits into the bacteria suspension, soaking the fruits for 30 seconds, immediately taking out the fruits, and naturally airing the fruits; and putting the fruits into a plastic basket, sealing the fruits by using a preservative film, and storing the fruits at room temperature or under a refrigerating condition. The method enhances the prevention and control effect of the rhodotorula mucilaginosa on the fruit postharvest diseases by using the phytic acid, and is simple in use, convenient for operation, good in effect and low in cost. The rhodotorula mucilaginosa with enhanced prevention and control effect can substitute a chemical bactericide to prevent and control the fruit postharvest diseases, so that harm of the chemical bactericide to the human body is avoided; and the method has remarkable economic benefit and social benefit.

Description

technical field [0001] The present invention relates to utilizing phytic acid (phytic acid) to enhance Rhodotorula coccidis strain ( Rhodotorula mucilaginosa ) is a method for preventing and controlling fruit postharvest diseases, and belongs to the field of fruit postharvest biological control. Background technique [0002] The loss of fruits due to rot in the process of picking, transportation and storage is huge, which has become a global concern. According to reports, the loss caused by this in developed countries is 10%-25%, while in developing countries lacking refrigeration facilities, it is as high as 40%-50%. The post-harvest loss of fruits in my country is about 20%-25%. The National Economic and Social Development Statistical Bulletin of the People's Republic of China in 2006 issued by the National Bureau of Statistics in March 2007 shows that in 2006, my country's total fruit output reached 170.5 million tons. According to this estimate, the loss of fruits afte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/155C12N1/16C12R1/645
Inventor 张红印赵利娜任晓锋张晓云代春华
Owner JIANGSU UNIV
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