Rhodotorula mucilaginosa, application thereof in preventing and curing postharvest disease of fruit and use method thereof

A technology for fruit postharvest disease and Rhodotorula japonicus, applied in the direction of microbial-based methods, applications, and preservation of fruits and vegetables, can solve problems affecting the use of antagonistic bacteria, and achieve the effects of reducing losses, inhibiting pathogenic bacteria, and preventing rot

Inactive Publication Date: 2010-11-24
无棣鑫岳化工集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These factors have affected the use of antagonistic bact...

Method used

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  • Rhodotorula mucilaginosa, application thereof in preventing and curing postharvest disease of fruit and use method thereof
  • Rhodotorula mucilaginosa, application thereof in preventing and curing postharvest disease of fruit and use method thereof
  • Rhodotorula mucilaginosa, application thereof in preventing and curing postharvest disease of fruit and use method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: Utilize Rhodotorula gelatin to carry out post-harvest disease prevention and storage and fresh-keeping methods of fruit (pome fruit and peach fruit)

[0029] The activated yeast was inoculated in a conical flask filled with 50ml of NYDB (5g of yeast extract, 8g of beef extract, 10g of glucose, 1000ml of purified water, pH7.0-9.0), and cultured on a shaker at 28°C and 180r / min for 48h. Take the bacterial solution and centrifuge it at 12000r / min for 10min, discard the supernatant, wash it repeatedly with sterile water for 3 times, and count it on a hemocytometer to obtain a concentration of 1×10 8 cells / ml of bacterial suspension. Punch 3 holes with a diameter of 3m (width) × 3mm (depth) at the waist of the fruit, and inject 30 μl of antagonistic bacteria at a concentration of 1 × 10 in each hole. 8 cells / ml cell suspension, the control fruit was injected with 30 μl sterile water. Two hours later, 20 μl of spore suspensions of Botrytis cinerea and Penicilliu...

Embodiment 2

[0033] Embodiment 2: Utilize Rhodotorula gum to carry out fruit (pome fruit) post-harvest disease prevention and storage method

[0034] 1. Test plan

[0035] The activated yeast was inoculated in a conical flask filled with 50ml of NYDB (5g of yeast extract, 8g of beef extract, 10g of glucose, 1000ml of purified water, pH7.0-9.0), and cultured on a shaking table at 28°C and 180r / min for 48h. Take the bacterial solution and centrifuge it at 12000r / min for 10min, discard the supernatant, wash it repeatedly with sterile water for 3 times, and count it on a hemocytometer to obtain a concentration of 1×10 8 cells / ml of bacterial suspension. Soak pome fruit to 1×10 8 In the bacterium suspension of each / ml, the control pome fruit is soaked in sterile water, and the time is 30 seconds-2 minutes. After drying, put the fruit into a plastic basket, seal it with plastic wrap to maintain a humidity of 95%, and store it at room temperature. After 45 days, the rot rate of the fruit was ...

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Abstract

The invention discloses Rhodotorula mucilaginosa which has efficacies of prevention and cure for postharvest disease of fruit, and application and use method thereof in storage and refreshment of fruits. The strain of the invention is CGMCC No.3617. When in use, Rhodotorula mucilaginosa is activated, cultured through fermentation and centrifuged to obtain thalluses, the thalluses are diluted by sterile water to prepare into bacterial suspension of 1.0*107-numbered thalluses/ml to 1.0*109-numbered thalluses/ml. Fruits are put into the bacterial suspension to soak for about 30s to 2min, then taken out immediately to perform air drying, put in a plastic crate, sealed by a preservative film, and then stored under room temperature or in cold storage. In the invention, antagonistic yeast is used to prevent and cure postharvest disease of fruit, the invention has simple use, convenient operation, good effect and low cost, thereby avoiding dangers to people or pollution to environment from using chemical bactericide, thus the invention has significant economic benefits and social benefits.

Description

technical field [0001] The invention relates to a strain of Rhodotorula mucilaginosa, the application of the strain to the prevention and treatment of post-harvest fruit diseases, and a method for using the strain. Background technique [0002] The loss caused by rot in the process of fruit picking, transportation and storage is huge, and it is also a problem of global concern. According to reports, in developed countries, 10% to 30% of fresh products are rotted due to postharvest diseases, while in developing countries that lack storage and transportation refrigeration equipment, the postharvest fruit rot rate is as high as 40% to 50%. The storage and preservation of foreign fruits accounts for more than 60% of the total fruit output. At present, the storage capacity of fruit in my country is more than 8 million tons, which is less than 15% of the total. Among them, the storage capacity of cold storage and air-conditioning storage is 700,000 tons, accounting for only 10% of...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23B7/155C12R1/645
Inventor 张红印黄星奕徐占利董英任晓锋
Owner 无棣鑫岳化工集团有限公司
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