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Glyphosate degrading bacterium and application of glyphosate degrading bacterium to compound pollution treatment of heavy metal-glyphosate

A heavy metal and glyphosate technology, applied in the field of microorganisms, can solve the problem of inability to effectively deal with heavy metal-glyphosate composite pollution, and achieve the effects of good degradation and good growth.

Active Publication Date: 2017-08-25
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned problem that the prior art cannot effectively deal with heavy metal-glyphosate complex pollution, the present invention aims to provide a heavy metal-resistant and highly efficient degradation of glyphosate waste water. A method for neutralizing glyphosate pollutants to solve the problem of microbial degradation of glyphosate in heavy metal polluted environments

Method used

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  • Glyphosate degrading bacterium and application of glyphosate degrading bacterium to compound pollution treatment of heavy metal-glyphosate
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  • Glyphosate degrading bacterium and application of glyphosate degrading bacterium to compound pollution treatment of heavy metal-glyphosate

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Embodiment 1

[0037] The screening of embodiment 1 Rhodotorula japonicus OP11

[0038] (1) Isolation and purification of strains

[0039] 1. Enrichment of bacterial strains: Collect 2.5g of farmland soil samples that have been applied with glyphosate for a long time, place them in a shake flask containing 60mL of enrichment medium, add 30mL of 100mM glyphosate and 5mM CuCl prepared in sterile water 2 10 mL of the solution was placed in a shaker at 30°C, 180 rpm, and incubated for 4 days with constant temperature shaking.

[0040]2. Acclimatization of bacterial strains: under aseptic conditions, add the bacterial solution to the inorganic salt basic liquid medium with glyphosate as the only carbon source at an inoculum size of 10%. Increased to 500mM, from 5mM, 10mM to 15mM. The total volume of the culture solution is 100mL, placed in a shaker, 30°C, 180rpm, constant temperature shaking culture, 4d is one acclimatization cycle, and three cycles of acclimatization are carried out.

[0041]...

Embodiment 2

[0051] Example 2 Tolerance and degradability of bacterial strain OP11 to glyphosate

[0052] Under sterile conditions, the purified strain OP11 was pre-cultured in a basal salt medium containing 200mM glyphosate for 24h (OD≈1.0), and the culture solution was inoculated into the basal salt medium with an inoculum volume fraction of 5%. , adding different concentrations (100, 250 and 500 mM) of glyphosate as a carbon source, respectively, 30 ° C, 180 rpm, shaking culture for 4 days. Samples were taken every 6 hours to determine the growth of bacteria and the degradation rate of glyphosate. Use an ultraviolet spectrophotometer to measure the OD value of the bacteria at a wavelength of 600 nm, and draw a growth curve. The concentration of glyphosate in the supernatant was determined by nitrosylation ultraviolet spectrophotometry.

[0053] Calculation method of degradation rate: degradation rate (%)=(1-C 1 / C 0 )×100. C 1 : Glyphosate residual concentration treated by degradi...

Embodiment 3

[0056] Degradation of glyphosate by bacterial strain OP11 under different pHs of embodiment 3

[0057] Under sterile conditions, the purified strain OP11 was pre-cultured in a basal salt medium containing 200mM glyphosate for 24h (OD ≈ 1.0), and the culture solution was inoculated to a pH of 5.0 according to an inoculum volume fraction of 5%. 6.0, 7.0, 8.0, 9.0 In the inorganic salt medium with 250mM glyphosate as the sole carbon source, 30°C, 180rpm, shake at constant temperature for 60h, then measure the degradation rate of glyphosate. The determination of the glyphosate degradation rate is the same as that of the implementation case 2.

[0058] Depend on Image 6 It can be seen that the strain OP11 grows in a wide pH range in the medium with glyphosate as the only carbon source, grows well in the range between pH 4.0 and pH 9.0, and has good tolerance to glyphosate. Degradation. The optimum pH range for strain OP11 to degrade glyphosate was pH5.0-7.0, and the degradation...

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Abstract

The invention relates to the field of microorganisms, in particular relates to a heavy metal-resistant glyphosate degrading bacterium (Rhodotorula mucilaginosa), OP11, preservation unit: China General Microbiological Culture Collection Center (CGMCC), preservation date: January 6, 2017, preservation number: CGMCC No. 13540. The heavy metal-resistant glyphosate degrading bacterium has the ability to use glyphosate as carbon and nitrogen source, has the ability to resist heavy metals, can rapidly absorb heavy metals, decompose and assimilate the glyphosate at the same time, and achieve the purpose of reducing the glyphosate in the environment.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a heavy metal-resistant glyphosate-degrading fungus Rhodotorula mucilaginosa and its degradation of glyphosate in a heavy metal-polluted environment. Background technique [0002] Glyphosate (N-phosphonic acid methyl-glycine) is a systemic broad-spectrum herbicide, which is widely used in agriculture because of its high efficiency and remarkable effect. However, studies in recent years have shown that long-term application of glyphosate will cause inestimable impacts on non-target organisms and the environment, especially on aquatic organisms and soil microorganisms. In addition, with the development of modern industry and agriculture, more and more heavy metals have entered the environment, and the frequent use of sewage irrigation, pesticides and fertilizers containing heavy metals has further deteriorated the environment. These production methods may lead to heavy me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C02F3/34C12R1/645C02F101/38C02F101/20C02F101/22
CPCC02F3/347C02F2101/20C02F2101/22C02F2101/306C02F2101/38C12N1/145C12R2001/645
Inventor 宋宁宁刘君王芳丽王凯荣李绍静卞建林
Owner QINGDAO AGRI UNIV
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