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Method for improving fermentation productivity of carotenoid

A carotene and yield technology, applied in the field of high antioxidant carotenoids preparation, can solve the problems of long production cycle, poor functionality, difficult extraction and the like, and achieve the effects of low production cost, short fermentation cycle and small footprint

Inactive Publication Date: 2015-08-19
WEIHAI LIDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, mainly using Haematococcus pluvialis to obtain natural astaxanthin is the main production method, but this method has a long production cycle, while the red yeast method is difficult to extract due to wall thickness and too high cis (3R-3R’) content, poor functionality

Method used

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  • Method for improving fermentation productivity of carotenoid
  • Method for improving fermentation productivity of carotenoid
  • Method for improving fermentation productivity of carotenoid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Using Rhodotorula gum as the carotenoid fermentation strain, inoculate the Rhodotorula gum into the culture medium according to the inoculum size of 5%. The culture medium includes: glucose 12g / L, yeast powder 2g / L, salt 3g / L, the pH of the medium is 5.5, add ferrous ion solvent to the medium, and maintain the fermentation temperature at 27°C. After 32 hours of fermentation, the shaker speed is 140r / min, and the temperature is lowered to 20°C to continue Fermentation, fermentation 24 hours, shaker speed 80r / min.

[0017] The preservation number of the Rhodotorula gum is CGMCC No.10123.

[0018] The ferrous ion solvent is ferrous sulfate, and the added amount of ferrous sulfate is 0.05mmol / L relative to the molar concentration of the medium.

Embodiment 2

[0020] Using Rhodotorula gum as the carotenoid fermentation strain, inoculate the Rhodotorula gum into the culture medium according to the inoculum size of 7%. The culture medium includes: glucose 130g / L, yeast powder 22g / L, salt 12g / L, the pH of the medium is 7.5, add ferrous ion solvent to the medium, the fermentation temperature is maintained at 29°C, after 40 hours of fermentation, the shaker speed is 200r / min, and the temperature is lowered to 25°C to continue Fermentation, fermentation 38 hours, shaker speed 140r / min.

[0021] The ferrous ion solvent is ferrous sulfate, and the added amount of ferrous sulfate is 0.5 mmol / L relative to the molar concentration of the medium.

Embodiment 3

[0023] Using Rhodotorula gum as the carotenoid fermentation strain, inoculate the Rhodotorula gum into the culture medium according to the inoculum size of 5%. The culture medium includes: glucose 71g / L, yeast powder 11g / L, salt 7.5g / L, the pH of the medium is 6.5, add ferrous ion solvent to the medium, the fermentation temperature is maintained at 30°C, after 36 hours of fermentation, the shaking table speed is 170r / min, and the temperature is lowered to 22°C Continue to ferment, ferment for 35 hours, shaker speed 110r / min.

[0024] The ferrous ion solvent is ferrous sulfate, and the added amount of ferrous sulfate is 0.275mmol / L relative to the molar concentration of the medium.

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Abstract

The invention discloses a method for improving the fermentation productivity of carotenoid, which is realized by the following steps: rhodotorula mucilaginosa is used as fermentation strains of the carotenoid, and the rhodotorula mucilaginosa is inoculated into a culture medium according to the inoculum size of 2%-10%; a ferrous ion solvent is added into the culture medium, wherein the volume of addition of the ferrous ion solvent is that the molarity relative to the culture medium is between 0.05-0.5mmol / L, and the fermentation temperature is kept at 27-33 DEG C; after the fermentation lasts for 32-40 hours, the temperature is lowered to 20-25 DEG C for continuous fermentation; the fermentation lasts for 24-46 hours. By adding ferrous ions, the capability of beta-carotene for converting into the high antioxygen carotenoid is greatly improved, the insufficience of quantity of beta-carotene dioxygen dismutase and beta-carotene hydrogenase is made up; the beta-carotene is converted into the high antioxygen carotenoid; by the method, the fermentation yield is improved by 30-34%.

Description

technical field [0001] The invention relates to the technical field of preparation of high antioxidant carotenoids, in particular to a method for improving the fermentation yield of carotenoids. Background technique [0002] Carotenoid and carotene are antioxidants and coloring agents widely used in food and health food, feed, cosmetics, etc., to improve human and animal health. Among them, astaxanthin is the strongest antioxidant discovered by humans in nature so far. It has anti-aging, anti-tumor, and cardiovascular and cerebrovascular disease prevention effects. It is widely used in health food, high-end cosmetics, pharmaceuticals, and feed industries. At present, mainly using Haematococcus pluvialis to obtain natural astaxanthin is the main production method, but this method has a long production cycle, and the red yeast method is difficult to extract due to the wall thickness and the cis (3R-3R') content is too high, and the functionality is poor. . Contents of the i...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
Inventor 魏升宁张文丽张美丽孙常明李纪丰程振伟
Owner WEIHAI LIDA BIOTECH
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