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Rhodotorula mucilaginosa DJHN070401 with high antimony resistance as well as separation screening method and application thereof

A technology of high antimony-resistant glue and yeast, which is applied in the field of microorganisms, can solve the problems of antimony-resistant microorganism screening, few identification methods, and weak related technologies, so as to reduce antimony toxicity and ecological hazards, good tolerance performance, and good antimony resistance performance effect

Active Publication Date: 2018-11-23
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The key to using microbial technology to control antimony pollution is to obtain strains with high resistance to antimony, but there are few screening and identification methods for antimony-resistant microorganisms, and related technologies are still weak

Method used

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  • Rhodotorula mucilaginosa DJHN070401 with high antimony resistance as well as separation screening method and application thereof
  • Rhodotorula mucilaginosa DJHN070401 with high antimony resistance as well as separation screening method and application thereof
  • Rhodotorula mucilaginosa DJHN070401 with high antimony resistance as well as separation screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and screening of Rhodotorula gum DJHN070401

[0027] The Rhodotorula japonicum of the present invention has a genus name of Rhodotorula, a species name of mucilaginosa, and a strain name of DJHN070401. The strain is isolated and screened from agricultural soil near the North Mine Smelter in Xikuangshan, Hunan, specifically through plate resistance screening. The screening method belongs to routine experimental methods in the art. The specific method and process are described as follows:

[0028] Take 10g of the soil sample to be tested, add it to a conical flask filled with 90mL of sterile water, stir it on a magnetic stirrer for 30min, let the strains be fully eluted from the soil sample, and let it stand for 20min; then take 1mL of the supernatant Add it to the improved 9K liquid medium (Wang Shuguang, Xu Zhe, Luo Pengcheng. An improved 9K solid medium preparation method [P].201510671854.7.), and cultivate in a shaker at 30°C and 150rpm for 1 to 2...

Embodiment 2

[0032] Molecular Identification of Example 2 Rhodotorula japonicus DJHN070401

[0033] In this example, the Rhodotorula glucosa DJHN070401 of the present invention was tested and analyzed through the 18S rRNA sequence, 26S rRNA sequence and ITS sequence respectively.

[0034] 2.1 Determination and analysis of 18S rRNA sequence of Rhodotorula gum DJHN070401

[0035]DNA extraction from fungi: Take an appropriate amount of bacterial samples and centrifuge at 8000r / m for 10min, discard the supernatant; add 100μl STEs to resuspend; add 80μl glass beads (diameter 0.1-0.2mm), add 40μl TE (pH=8.0) to each tube; add 120μl PCI (phenol:chloroform:isoamyl alcohol=25:24:1); vortex for 10min; centrifuge at 12000r / m for 10min, take the upper aqueous phase into a new EP tube, add an equal volume of isopropanol, and then add 1 / 5 volume NaAc; Precipitate at -20°C for 15 minutes, centrifuge at 12,000 r / m at 4°C for 10 minutes; discard the supernatant, fully dry the DNA in air at 37°C, dissolve ...

Embodiment 3

[0062] Example 3 Antimony Performance Test of Rhodotorula japonicus DJHN070401

[0063] The isolated pure culture strain was inoculated into 100 mL of 9K liquid medium, and cultured with shaking at 30°C overnight. Dilute the bacterial concentration to 10 -2 After that, take 60 μL and apply it to solids with Sb(Ⅴ) concentrations of 100, 200, 300, 500, 750, 1 000 mg / L and Sb(Ⅲ) concentrations of 50, 100, 150, 200, 300, 400, 500 mg / L in sequence. On the medium plate, make 3 parallel plates for each antimony concentration gradient. After culturing at a constant temperature of 30°C for 3 days, the growth of the colony was observed. The results showed that the isolated pure culture strains were able to grow on solid medium plates with Sb(Ⅴ) concentrations of 100, 200, 300, 500, and 750 mg / L respectively, but colonies on solid medium plates with 750 mg / L The number decreased significantly, indicating that the concentration of Sb(Ⅴ) was 750mg / L, which was close to the limit concent...

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Abstract

The invention relates to rhodotorula mucilaginosa DJHN070401 with high antimony resistance as well as a separation screening method and application thereof, and belongs to the technical field of microorganisms. A strain of the rhodotorula mucilaginosa is classified and named as rhodotorula mucilaginosa DJHN070401, and is preserved in China Center For Type Culture Collection (CCTCC), wherein the preservation number is CCTCC NO: M2018212, and the preservation date is April, 18th, 2018. The strain of the rhodotorula mucilaginosa disclosed by the invention is obtained through performing separationscreening agricultural soil near to northern ore smelting plants of a tin mine in Hunan province. The rhodotorula mucilaginosa DJHN070401 disclosed by the invention has higher adsorption volume and high adsorption speed on antimony ions in a water body, is environmentally friendly, has good application prospect, and has significance on development of a technology for performing adsorption removalon antimony based on microorganisms.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a rhodotorula colloides, more specifically, the invention relates to a high antimony-resistant rhodotorula colloids DJHN070401 and its separation and screening method and application. Background technique [0002] Antimony (Sb) is a metalloid element in the same main group as arsenic. It is listed as a priority pollutant for its genotoxicity, carcinogenicity, and global mobility. Collection library. Therefore, how to make antimony-containing wastewater meet the standard before discharge is an urgent scientific task. Traditional antimony-containing wastewater treatment technologies at home and abroad include chemical coagulation precipitation, electrochemical precipitation, chemical redox, ion exchange, membrane treatment technology, etc. Although these technologies have certain positive effects, they still have low efficiency, high cost, and operational problems. Complex, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/02B09C1/10C02F3/34C12R1/645C02F101/20
CPCB09C1/105C02F3/347C02F2101/20C12N1/02C12N1/16C12N1/145C12R2001/645
Inventor 邓仁健金昌盛唐智娥黄中杰侯保林任伯帜
Owner HUNAN UNIV OF SCI & TECH
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