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Method for preparing compound microorganism fermented forage feed

A compound microorganism and forage technology, which is applied in the field of compound microorganism fermented forage forage preparation, can solve the problems of uneven product quality, difficulty in ensuring the safety of product feed, and large-scale production not yet formed.

Active Publication Date: 2012-07-25
新疆瑞源乳业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and application of fermented pasture feed in China is still in its infancy, most of which are naturally fermented workshop-style silage, which has not yet formed a large-scale production, the product quality is uneven, and the safety of product feeding is difficult to guarantee

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The 4 strains used in the present invention were all purchased from the China Industrial Microorganism Culture Collection and Management Center, namely: Trichoderma konningen CICC13037, Rhodotorula colloides CICC 31192, Bacillus subtilis CICC 10063, and Bacillus subtilis CICC 10089.

[0038] 1. Preparation process of compound microbial starter freeze-dried powder

[0039] 1.1 Activation of the original strain

[0040] Measure 10ml of 0.9% normal saline into 4 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacterial powders in the ampoules of 4 strains into 0.9% In normal saline, oscillate to make it dissolve, and activate it in a 30°C incubator for 30 minutes, and set aside.

[0041] 1.2 Expanded culture of fermentation bacteria

[0042] 1.2.1 Preparation of mother starter

[0043] Measure 200ml of culture medium for each of the four strains into four 500ml Erlenmeyer flasks, sterilize at 121°C for 20 minutes and cool to 30°...

Embodiment 2

[0060] 1. Preparation process of compound microbial starter freeze-dried powder

[0061] 1.1 Activation of the original strain

[0062] Measure 10ml of 0.9% normal saline into 4 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacterial powders in the ampoules of 4 strains into 0.9% In normal saline, oscillate to make it dissolve, and activate it in a 30°C incubator for 30 minutes, and set aside.

[0063] 1.2 Expanded culture of fermentation bacteria

[0064] 1.2.1 Preparation of mother starter

[0065] Measure 200ml of culture medium for each of the four strains into four 500ml Erlenmeyer flasks, sterilize at 121°C for 20 minutes and cool to 30°C, inoculate 10% of the volume of the culture medium with the activated strains in step 1.1, at 30°C, The shaking table was cultivated for 24 hours under the condition of shaking table speed of 110 rpm, as the mother starter.

[0066] 1.2.2 Preparation of production starter

[0067] First, ...

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Abstract

The invention provides a method for preparing a compound microorganism fermented forage feed. The method is characterized by firstly preparing a compound microorganism fermentation agent lyophilized powder of trichoderma koningii CICC 13037, rhodotorula mucilaginosa CICC 31192, bacillus subtilis CICC 10063 and bacillus subtilis CICC 10089, activating the lyophilized powder with 2% of glucose solution and then inoculating the activated lyophilized powder into the fresh forage section, then carrying out insulation fermentation and aeration-drying on the forage and then packaging the forage, thus obtaining the finished product.

Description

(1) Technical field [0001] The invention relates to a preparation method of compound microorganism fermented grass feed. (2) Background technology [0002] The development of pasture feed fermentation has a long history, and all countries in the world attach great importance to the research and production of fermented pasture feed. The research and application of fermented pasture feed in China is still in its infancy. Most of them are naturally fermented workshop-style silage, which has not yet formed a large-scale production. The quality of the products is uneven, and the safety of the products for feeding is difficult to guarantee. [0003] The so-called biological feed is a safe and pollution-free bacterial feed containing active probiotics made by mixing known beneficial microorganisms and nutrient carriers under the guidance of microecological theory through special processes such as fermentation and drying. Microorganisms can generally grow and reproduce using simple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14
Inventor 陈成滕春红姜帆佟晓芳刘文辉
Owner 新疆瑞源乳业有限公司
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