Chryseobacterium and application thereof in preparation of halophyte epiphytic repair and maintenance microbial inoculum

A technology of Chryseobacterium aureus and halophytes, applied in plant growth regulators, plant growth regulators, methods based on microorganisms, etc., can solve the problems of easy diffusion and loss, difficult to multiply and repair, and achieve less bacterial agent consumption , promote growth, promote the effect of plant growth

Pending Publication Date: 2022-01-21
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to the strong hydrodynamic effect of the ocean, conventional bacterial agents are easy to spread and lose in the process of repairing sea areas, and it is difficult to multiply and play a repairing role

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Separation and identification of Gold Bacillus

[0038] Separation of strains

[0039] From the coastal wetland, the base is collected from the river, and the mass ratio of 2% is transferred to a sterilized enrichment medium, and after 1-7 days of oscillating, dilution culture is carried out in LB solid medium, and selecting a single colony Separation is carried out on agar solid medium, and the purified strain YTLJ-E-II12 is obtained.

[0040] The enrichment medium is from 0.05 to 0.2% mass (NH) 4 ) SO 4 0.2-1% sodium succinate, 2-5% K 2 HPO 4 1-3% MGSO 4 , 1-3% NaCl, 0.01-0.05% FESO 4 0.01-0.05% MNSO 4 0.001-0.005% (NH 4 ) 2 MOO 4 , 0.001-0.005% CoCl 2 0.1-1% Bacterial population induction inhibitor (specifically chlorozoranone), 0.1-1% bisphenol A and 0.02-0.1% high carbon alcohol fatty acid ester complex.

[0041] 2, identification of strains

[0042] 2.1, morphological identification

[0043] The above step 1 was separated and purified to the obtained strain for analys...

Embodiment 2

[0052] Separation and identification of Obsuscus YTLJ-N-L43

[0053] Separation of strains

[0054] The collection of seaweed with seaweed is transferred to a sterilized enrichment medium by mass ratio of 1%, and after 1-7 days of shocking, dilution culture is carried out in LB solid medium, and single colonies fall in agar solids. The medium is separated on the medium, and the purified strain YTLJ-N-L43 is obtained.

[0055] The enrichment medium is from 0.05 to 0.2% mass (NH) 4 ) SO 4 0.2-1% sodium succinate, 2-5% K 2 HPO 4 1-3% MGSO 4 , 1-3% NaCl, 0.01-0.05% FESO 4 0.01-0.05% MNSO 4 0.001-0.005% (NH 4 ) 2 MOO 4 , 0.001-0.005% CoCl 2 0.1-1% Bacterial population induction inhibitor (specifically phtolinol), 0.1-1% bisphenol A and 0.02-0.1% high carbon alcohol fatty acid ester complex.

[0056] 2, identification of strains

[0057] 2.1, morphological identification

[0058] The above step 1 was separated and purified to the obtained strain for analysis and compared the bacterial s...

Embodiment 3

[0066] 1) Gold pylori YTLJ-E-II12 to the Philipphi Pollution Wastewater Treatment:

[0067] The goldenum paconium YTLJ-E-II12 obtained by the above-described embodiment is added to 1% inoculation amount to a pneryless pollution wastewater, which has a concentration of the waste reaches 20 μg / L. The introduction of goldenumi YTLJ-E-II12 was decreased by 30 ° C for 30 days, and the Philippine removal rate reached 96%.

[0068] 2) Treatment of high-amamine nitrogen breeding wastewater by Cushifa YTLJ-N-N-L43:

[0069] The altoma YTLJ-N-L43 obtained by the above embodiment was added to aquaculture wastewater by 1% inoculation, and the ammonia nitrogen concentration in wastewater reached 10 mg / L. The introduction of the femoraliostel ytlj-N-L43 treatment of aquaculture wastewater at 25 ° C, and the ammonia-removal rate of 7 days can reach 96%.

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Abstract

The invention relates to a halophyte epiphytic repair and maintenance microbial inoculum, which is prepared by the following steps: respectively pre-culturing and propagating Chryseobacterium YTLJ-E-II12 and Acinetobacter YTLJ-N-L43, carrying out vacuum freeze drying to obtain corresponding single-bacterium powder, mixing the single-bacterium powder, and mixing with halophyte extract and the like to obtain the halophyte epiphytic repair and maintenance microbial inoculum. The Chryseobacterium YTLJ-E-II12 and the Acinetobacter YTLJ-N-L43 are preserved in the Guangdong Microbial Culture Collection Center, and the preservation numbers are respectively GDMCC 61770 and GDMCC 61691. The microbial inoculum can strengthen the combined removal of various toxic pollutants in the high-salt environment, has the effect of promoting the growth of halophyte, and is used for treating the organic-inorganic combined pollution of the high-salt environment such as ocean and coastal zones.

Description

Technical field [0001] The present invention relates to a method in the field of environmentally friendly techniques, and in particular, a goldenbacterus and an application in the preparation of salt plants. Background technique [0002] With the rapid development of the economy and the enhancement of human activities, environmental pollution has become an important factor in restricting the development of coastal vulnerability. The coastal environment is called Luyuan Natural and Synthetic Chemical's "Terminal Try", which has been detected in a variety of coastal environments (domestic sewage, treatment plants, seawater, surface water and groundwater) to detect NG L -1 To MGL -1 Chemical pollutants. At this stage, the biomaffore technology is considered to be one of the most cost effective methods of removing organic pollutants and rushed nitrogen phosphorus nutrient elements in water bodies. It has low cost and does not cause secondary pollution of the environment. . While bior...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C02F3/34C02F3/32C02F3/30C05F11/08A01N63/20A01P21/00C12R1/01C02F101/16C02F101/30
CPCC12N1/20C12N1/36C02F3/34C02F3/325C02F3/32C02F3/303C02F3/305C05F11/08A01N63/20C02F2101/16C02F2101/166C02F2101/163C02F2101/30
Inventor 吕剑武君王娜王建华张翠
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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