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COD (chemical oxygen demand) degrading strain and application thereof

A liquid bacterial agent, the technology of aureobacillus indole, applied in the field of environmental microorganisms, can solve the problems of death of aquatic organisms, impact on environmental sanitation, water quality deterioration, etc., and achieve the effects of high application value, good economic benefits, and high treatment efficiency.

Active Publication Date: 2020-10-02
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If this kind of wastewater is not treated properly and discharged directly, due to biodegradation, the receiving water body will be hypoxic, most aquatic organisms will die, the water quality will deteriorate day by day, odor will be generated, and environmental sanitation will be affected. Disease microorganisms will also multiply in large numbers, and a large amount of toxic organic substances will continue to accumulate and store in natural environments such as water bodies and soils, and finally enter the human body, endangering human health

Method used

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  • COD (chemical oxygen demand) degrading strain and application thereof
  • COD (chemical oxygen demand) degrading strain and application thereof
  • COD (chemical oxygen demand) degrading strain and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0056] The preparation method of the liquid bacterial agent is: producing Chryseobacterium indole Chryseobacterium indologenes The pure strain of CI-B4 was inoculated into LB liquid medium and cultured for 16-24 h.

[0057] Specifically, the culture temperature is 30°C.

[0058] Specifically, the rotating speed of the shaker during cultivation is 160-200 r / min.

[0059] A third aspect of the present invention provides indole-producing Chryseobacterium Chryseobacterium indologenes Application of CI-B4 or the liquid bacterial agent in COD degradation of organic wastewater.

[0060] Indole-producing Chryseobacterium indole Chryseobacterium indologenes The concentration of CI-B4 is at least 1×10 8 cfu / mL.

[0061] The organic wastewater includes livestock and poultry breeding wastewater, industrial organic wastewater, biogas slurry from anaerobic digestion of kitchen waste, black and odorous river water, domestic wastewater, and the like.

[0062] A fourth aspect of the p...

Embodiment 1

[0080] Example 1: Isolation, screening and performance measurement of COD degrading bacteria

[0081] Enrichment culture: The organic wastewater of the experimental sample was collected from various sewage treatment tanks from different sources. Take 5 mL of organic wastewater into 45 mL of sterilized wastewater liquid culture medium, mix it evenly and place it on a shaker at 30 °C and 180 r / min Medium enrichment culture for 96h.

[0082] Separation and purification: after gradient dilution of the cultured enrichment solution, evenly spread it on the waste water solid medium, cultivate it in a constant temperature incubator at 30°C for 24~48 hours, pick out the single clones with different colony shapes, and streak After purification, it is numbered and preserved. A total of 16 strains were obtained through separation and purification.

[0083] Preliminary verification: Inoculate the 16 isolated strains into LB liquid medium for enrichment culture, take 5% (volume ratio) of ...

Embodiment 2

[0094] Embodiment 2: The degradation effect of COD degrading bacteria CI-B4 on COD in livestock and poultry breeding wastewater

[0095] The wastewater from livestock and poultry farming is taken from the solid-liquid separation of manure from a cattle farm and a pig farm. The COD concentration of the stock solution of cattle breeding wastewater is 22000 mg / L, and the pH value is 7.5. The COD concentration of the stock solution of pig breeding wastewater is 18000 mg / L, pH value is 7.3.

[0096] The strain CI-B4 was inoculated in LB liquid medium, and cultured in a shaker at 30 °C and 180 r / min for 24 h. According to the volume ratio of 5%, it would be in the logarithmic growth phase (the bacterial density was at least 1×10 8 cfu / mL) was centrifuged, washed, and put into the reactor. The reactor was filled with 2 L of waste water. The control conditions were: the speed of the electric stirrer was 180 r / min, the DO value was 5 mg / L, and the temperature was 30 °C. , Adjust the ...

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Abstract

The invention relates to the technical field of environmental microorganisms, and particularly relates to a COD (chemical oxygen demand) degrading strain and application thereof. The COD degrading strain is identified as Chryseobacterium indologenes, and the preservation number is CCTCC NO: M 2018651. The Chryseobacterium indologenes CI-B4 is salt-resistant and low-temperature-resistant, and has awide temperature application range (5-40 DEG C); the Chryseobacterium indologenes CI B4 has the capability of efficiently degrading organic pollutants in wastewater, and can improve the effluent quality of organic wastewater; after enlarged culture, the Chryseobacterium indologenes CI-B4 can be applied to biochemical treatment of livestock and poultry breeding wastewater, industrial organic wastewater, black and odorous water bodies of rivers, anaerobic digestion biogas slurry of kitchen waste and the like, and has a high application value; and when the Chryseobacterium indologenes CI-B4 is used for treating organic wastewater, the treatment efficiency is high, the economic benefit is good, the operation is convenient, and pollution is avoided.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a COD-degrading strain and application thereof. Background technique [0002] With the acceleration of the global industrialization process, organic pollution of the water environment has become one of the global environmental protection issues. Organic pollutants mainly come from the discharge of large-scale high-concentration organic wastewater, mainly from industry, agriculture and other fields. High-concentration organic wastewater mainly refers to wastewater whose chemical oxygen demand (Chemical Oxygen Demand, COD) reaches or exceeds thousands or even tens of thousands of milligrams per liter. The components of pollutants in high-concentration organic wastewater are complex, and they pose a serious threat to human health and the ecological environment after being discharged into water bodies. Therefore, the research on high-concentration organic waste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F103/20C02F101/30C12R1/01
CPCC02F3/34C02F2101/30C02F2103/007C02F2103/20C02F2209/02C02F2209/06C02F2209/08C02F2209/22C02F2303/02C12N1/20C12N1/205C12R2001/01
Inventor 刘莉王娜史吉平颜薇芝李泳洪陈晓淼梁承宇
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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