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Biological preparation containing plant source component and microorganism source component and application of biological preparation in prevention and treatment of plant nematode diseases

A microbial source, biological preparation technology, applied in plant growth regulators, microorganism-based methods, microorganisms, etc., can solve the problems of unsatisfactory control effect and unstable control effect of single plant-derived preparations

Active Publication Date: 2021-10-01
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research on biological control of root-knot nematode mainly focuses on the control of single components. The control effect of a single plant source preparation is unstable, while a single bacterial agent has the effect of controlling root-knot nematode, but the control effect is not ideal. disease control challenges

Method used

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  • Biological preparation containing plant source component and microorganism source component and application of biological preparation in prevention and treatment of plant nematode diseases
  • Biological preparation containing plant source component and microorganism source component and application of biological preparation in prevention and treatment of plant nematode diseases
  • Biological preparation containing plant source component and microorganism source component and application of biological preparation in prevention and treatment of plant nematode diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Screening method and identification method of cold-resistant psesson strain SJ5

[0046] 1. Separation and screening of the biopsy

[0047] Separation and screening of cold sputum strain SJ5 using dilution plate coating method, the specific steps are as follows: Separation of cyclic soil bacteria in the pathogeneous tomato, take 100 μl of dilutions on NA medium, each gradient 3 The repetition was cultured at 30 ° C for 40 hours, and the single colonies different from the form of different morphological characteristics were observed. After purification, it was stored in the 4 ° C refrigerator. Splitting, purified strains were screened in NA medium, and the strain was seeded with Na medium, and after 72 h at 27 ° C, the bacterial morphology was observed, and the SJ5 colony was milky white, circular, bright, micro-transparent (See figure 1 ). Gram-stained results showed that the form of the SJ5 strain was rod, no spores, and the SJ5 strain showed fresh red, which proves that t...

Embodiment 2

[0059] Preparation method of three microbial fermentation fluids

[0060] 1) The LB liquid medium is prepared, 20 min after 121 ° C, to obtain a sterilized LB liquid medium, and after adjusting the pH to 7.4, the LB liquid medium is separated into the shake flask, and 300 ml of medium per bottle. Cooling spare.

[0061] 2) Incoke 1ml to the shake flanet of the hematococcal strain SJ5 strain, inoculated on the shaker after the shaker was placed on the shaker, and 60 h was cultured at 180 rpm, 28 ° C, and the bacterial liquid was collected to obtain a cold pseudomonas strain. Fermentation of SJ5.

[0062] 3) Inoculation of the bond vagobacterium inoculated 1ml to the shake flask, the inoculated shake flask was placed on a shaker, cultured at 200 rpm, 30 ° C for 54 h, and the bacterial liquid was collected, and the fermentation broth of the bonding bunus was obtained.

[0063] 4) Inoculation of the Bacillus strain inoculation, in the shake flask, the inoculated shake flask was placed...

Embodiment 3

[0065] Toxicity assay method of three microbial fermentation fluid on southern roots

[0066] 1) The three microbial fermentation fluids prepared in Example 2 were filtered through a sterile filtration membrane (filtration film pore diameter 0.22 μm), respectively, and the fermentation filtrate of three microorganisms was obtained.

[0067] 2) Set five processes, set 5 petri dishes (35mm diameter) per handle, 20 μl of the roots (J2) suspension (J2) suspension (approximately 100 pieces), each dish add benefit to the final concentration of 100 μg / ml, The addition of mastin to a final concentration of 20 μg / ml, adding streptomycin to a final concentration of 50 μg / ml. Treatment 1 is 3000 μLB liquid medium (control group); 23000 μL strain SJ5 fermentation filtrate; 33000 μL of bispylobacterium fungus fermentation filtrate; 43000 μL of Bacillus fermentation filtrate; 53000 μL of bitter printer inducery oil dilution (1000 times, mother liquor concentration 1%) . 12h was placed in ...

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Abstract

The invention provides a biological preparation containing a plant source component and a microorganism source component and application of the biological preparation in prevention and treatment of root knot nematode diseases, which belongs to the technical field of biological pesticides. The invention provides a cold-resistant pseudomonas strain SJ5 with the preservation number of CCTCC No.M2021375, and the cold-resistant pseudomonas strain SJ5 has a relatively strong killing effect on nematodes. Meanwhile, the invention further provides a biological preparation containing plant source components and microorganism source components. The biological preparation is prepared from sophora flavescens azadirachtin missible oil, fermentation liquor of a cold-resistant pseudomonas strain SJ5, fermentation liquor of Chryseobacterium mucilaginosus and fermentation liquor of bacillus subtilis. The biological agent is used for preventing and treating plant nematode diseases, the biological prevention effect of chemical agents such as fosthiazate and dazomet can be achieved, plant growth can be effectively promoted, and the biological agent has higher application value.

Description

Technical field [0001] The present invention belongs to the technical field of biological pesticides, particularly relates to a biological preparation comprising a plant-derived component and a microorganism source component and its application in the prevention of root knot nematode. Background technique [0002] Plant parasitic nematodes from plant parasitic nematodes and plant diseases caused. There are agricultural control, chemical control traditional control methods of root-knot nematodes. Agricultural control such as deep plowing, crop rotation, cultivation of resistant varieties, but because of root-knot nematode host range is very wide, and the lack of domestic high resistance crop varieties, agricultural control effect is not ideal. Currently nematode products on the market is still dominated chemicals, fosthiazate and dazomet and other common chemicals capacity in the market share in a total of 69.12 percent, the extensive use of chemical pesticides leads to soil degra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N65/26A01N63/27A01N63/22A01N63/20A01P5/00C12R1/38
CPCA01N65/26A01N65/00A01N63/27A01N63/22A01N63/20Y02A50/30
Inventor 陈穗云舒洁张仁军
Owner YUNNAN UNIV
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