Feather degrading bacterium immobilization method
A technology for degrading bacteria and feathers, applied in the field of microorganisms, can solve the problems of threatening human and poultry health, easy breeding of pathogenic microorganisms, waste of protein resources, etc., and achieve the effects of low production cost, increased maintenance time, and easy recycling
Examples
Embodiment 1
[0043] The immobilized preparation process of feather degrading bacteria is as follows:
[0044] (1) Bacillus aureus was inoculated into LB liquid medium, and cultured at 30°C for 24 h to obtain a fermentation broth rich in Bacillus aureus.
[0045] (2) Using straw as a carrier, air-dried naturally, then crushed to 1 cm, washed with distilled water to remove impurities, and then sterilized at 121 °C for 30 min.
[0046](3) Then the carrier obtained in step (2) was added to (1) at 15 g / L, shaken at 30°C for 24 hours, and then centrifuged at 8000 rpm, discarded the supernatant, washed with sterile water and filtered.
[0047] (4) Add the filtered inoculum to the small petri dish, lay a flat layer, then put the small petri dish into the large petri dish, and place 3mm glass beads in the outer circle of the large and small petri dish. Wet the glass beads with distilled water, add 5ml / g of methyl orthosilicate dropwise evenly on the glass beads, then cover the large petri dish and...
Embodiment 2
[0050] The immobilized preparation process of feather degrading bacteria is as follows:
[0051] (1) Bacillus aureus was inoculated into LB liquid medium, and cultured at 37°C for 20 h to obtain a fermentation broth rich in Bacillus aureus.
[0052] (2) Using wood chips as a carrier, air-dried naturally, then crushed to 1 cm, washed with distilled water to remove impurities, and then sterilized at 121 °C for 30 min.
[0053] (3) Then the carrier obtained in step (2) was added to (1) at 25 g / L, shaken at 37°C for 24 hours, and then centrifuged at 8000 rpm, discarded the supernatant, washed with sterile water and filtered.
[0054] (4) Add the filtered inoculum to the small petri dish, lay a flat layer, then put the small petri dish into the large petri dish, and place 3mm glass beads in the outer circle of the large and small petri dish. Wet the glass beads with distilled water, add 3 ml / g of methyl orthosilicate dropwise evenly on the glass beads, and then cover the large pet...
Embodiment 3
[0057] The immobilized preparation process of feather degrading bacteria is as follows:
[0058] (1) Bacillus aureus was inoculated into the LB liquid medium, and cultured at 25°C for 20 h to obtain a fermentation broth rich in Bacillus aureus.
[0059] (2) Using straw as a carrier, air-dried naturally, then crushed to 1 cm, washed with distilled water to remove impurities, and then sterilized at 121 °C for 30 min.
[0060] (3) Then the carrier obtained in step (2) was added to (1) at 15g / L, shaken at 37°C for 24 hours, and then centrifuged at 8000 rpm, discarded the supernatant, washed with sterile water and filtered.
[0061] (4) Add the filtered inoculum to the small petri dish, lay a flat layer, then put the small petri dish into the large petri dish, and place 3mm glass beads in the outer circle of the large and small petri dish. Wet the glass beads with distilled water, and then evenly drop 1 ml / g of methyl orthosilicate on the glass beads, then cover the large petri di...
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