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A kind of petroleum degrading bacterial agent and its preparation method and application

A technology of oil-degrading bacteria and oil pollution, which is applied to the restoration of contaminated soil fixed on/in it, and can solve the problems of limited storage time, affecting repair efficiency, and different effects, and achieves convenience for transportation and Storage, strong environmental adaptability, and the effect of maintaining activity

Active Publication Date: 2018-05-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The degradation process of microorganisms to oil pollution has different effects under different environmental conditions, which affects the repair efficiency; and the bacterial liquid used in practical applications is fermented with rich nutrients, which has limited storage time and inconvenient transportation. question

Method used

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  • A kind of petroleum degrading bacterial agent and its preparation method and application
  • A kind of petroleum degrading bacterial agent and its preparation method and application
  • A kind of petroleum degrading bacterial agent and its preparation method and application

Examples

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preparation example Construction

[0024] A kind of preparation method of petroleum degrading bacterial agent provided by the invention, concrete steps are as follows:

[0025] 1) Dissolve polyvinyl alcohol completely at 80-100°C, and mix it with sodium alginate solution in proportion to obtain a mixed solution of sodium alginate and polyvinyl alcohol;

[0026] 2) Add activated carbon to the mixed solution of sodium alginate and polyvinyl alcohol and mix until the concentration of activated carbon is 5 g / L;

[0027] 3) After cooling the solution obtained in step 2) to 40°C, mix it with the oil-degrading bacteria solution at a volume ratio of 10:1;

[0028] 4) Under stirring conditions at 0~4°C, use a syringe to drop the solution obtained in step 3) into a calcium chloride saturated boric acid solution with a concentration of 30% to form a ball;

[0029] 5) Put the solution obtained in step 4) in a refrigerator at 4°C for 26 hours, then take out the microspheres in the solution and wash them with sterilized 0.9...

Embodiment 1

[0038] A kind of preparation method of petroleum degrading bacterial agent in the present embodiment, concrete steps are as follows:

[0039] 1) Dissolve polyvinyl alcohol completely at 80~100°C, mix it with sodium alginate solution in proportion to obtain a mixed solution of sodium alginate and polyvinyl alcohol; mix sodium alginate (SA) with polyvinyl alcohol (PVA) The mass percentage of sodium alginate in the solution is 1%, the mass percentage of polyvinyl alcohol is 8%, SA:PVA=1:8;

[0040] 2) Add activated carbon to the mixed solution of sodium alginate and polyvinyl alcohol and mix until the concentration of activated carbon is 5 g / L;

[0041] 3) After cooling the solution obtained in step 2) to 40°C, mix it with the petroleum-degrading bacteria solution at a volume ratio of 10:1; the petroleum-degrading bacteria solution contains Chryseobacterium, Caulobacter, Achromobacter and Burkholderia Bacterial solution, the mass ratio of the four strains is 36:22:18:5, the OD o...

Embodiment 2

[0051] In this example, the mass percentage of sodium alginate (SA) and polyvinyl alcohol (PVA) in the mixed solution of sodium alginate (SA) and polyvinyl alcohol (PVA) in step 1) is 2%, the mass percentage of polyvinyl alcohol is 8%, SA:PVA=2 : 8; all the other steps are identical with embodiment 1, prepare bacterial agent.

[0052] The mass percentage of petroleum in the petroleum-contaminated soil treated with the bacterial agent was 32%. In this example, the degradation rate of the oil-contaminated soil by the petroleum hydrocarbon-degrading bacterial agent was 13%, and the oil content in the soil changed as figure 2 shown.

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Abstract

The invention discloses a petroleum-degrading bacterial agent and its preparation method and application. The preparation method is as follows: mix polyvinyl alcohol and sodium alginate solution uniformly in proportion, then add activated carbon to mix evenly, and add 10:1 alginate after cooling. The ratio is mixed with the oil-degrading bacterial solution containing Chryseobacterium: Caulobacter: Achromobacter: Burkholderia (the mass ratio of each strain is 36:22:18:5); then use a syringe to drop the resulting mixed solution into chlorine Calcium chloride is saturated with boric acid solution to form balls; after standing still, the microspheres in the solution are taken out and washed with sterilized 0.9% NaCl solution to obtain a solid microsphere-shaped petroleum-degrading bacterial agent. The petroleum-degrading bacterial agent of the invention can be used to restore petroleum-contaminated soil; the form of the solid bacterial agent is also convenient for transportation and storage, and provides technical support for the practical application of microbial restoration of petroleum-contaminated soil.

Description

technical field [0001] The invention relates to the preparation of a mixed bacterial agent for repairing petroleum-contaminated soil, in particular to a petroleum-degrading bacterial agent and its preparation method and application. Background technique [0002] Petroleum is one of the most important energy sources in modern society, known as "the blood of industry" and "black gold". Petroleum is widely used in industrial fields such as the preparation of natural gas, gasoline, kerosene, volatile oil, lubricating oil, paraffin and asphalt. During the processing, use and transportation of crude oil, it is easy to cause spills and leakage accidents, causing oil to enter water bodies, soil and other environments to cause oil pollution. Petroleum is a complex mixture composed of various hydrocarbons and a small amount of non-hydrocarbon compounds. It is difficult to degrade in the environment. The polycyclic aromatic hydrocarbons in its components are a kind of persistent organ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10C12N11/08C12N11/04B09C1/10
Inventor 赵和平孟帆钟亮温丽莲张吟
Owner ZHEJIANG UNIV
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