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Preparation method and application of microbial immobilized composite particles

A technology of microbial immobilization and composite particles, which is applied in the field of environmental bioremediation, can solve the problems of polyvinyl alcohol adhesion and poor mass transfer performance, and achieve the effects of improving removal effect, promoting adsorption and decomposition, and increasing specific surface area

Pending Publication Date: 2022-05-27
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immobilization technology used in the present invention can form uniform holes inside the carrier, form a mass transfer channel on the surface of the carrier, and greatly improve the mass transfer performance of the particles; in the present invention, polyvinyl alcohol is used in combination with sodium alginate (SA), It can solve the problems of polyvinyl alcohol adhesion and poor mass transfer performance, and is beneficial to the preparation of pellets and immobilized particles; at the same time, adding biochar can improve the mechanical properties of the carrier, increase the specific surface area and adsorption sites of the carrier, and significantly Improved degradation of neonicotinoid pesticides

Method used

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  • Preparation method and application of microbial immobilized composite particles
  • Preparation method and application of microbial immobilized composite particles
  • Preparation method and application of microbial immobilized composite particles

Examples

Experimental program
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Effect test

Embodiment 1

[0053] A preparation method of microbial immobilized composite particles:

[0054] S1. After washing and drying the rice husks with deionized water, put them in a crucible and place them in a tube furnace. Under an argon atmosphere, react at 700° C. for 3 hours. After cooling to room temperature, take out the rice husks, and then use 0.1 mol of ·L -1 HCl and HNO 3 The mixed solution is washed until the lotion is neutral, the rice husks are dried, and then ground through a 100-mesh sieve to obtain biochar;

[0055] S2. Inoculate the Bacillus aureus strain in 100mL LB liquid medium, put it into a shaker for constant temperature cultivation (30°C, 160rpm), cultivate to the late logarithmic growth phase, and centrifuge the medium at room temperature (×4000g, 10min), Then washed with sterile water, resuspended in 0.9% physiological saline to obtain a bacterial suspension with an OD600 value of 0.75, and then added 0.6 g of the biochar to 50 mL of the bacterial suspension, and all...

Embodiment 2

[0061] A preparation method of microbial immobilized composite particles:

[0062] S1. After washing and drying the moso bamboo with deionized water, put it in a crucible and place it in a tube furnace. Under an argon atmosphere, react at 850° C. for 2 hours. After cooling to room temperature, take out the rice husk, and then use 0.1 mol· L -1 HCl and HNO 3 The mixed solution is washed until the lotion is neutral, the rice husks are dried, and then ground through a 100-mesh sieve to obtain biochar;

[0063] S2. Inoculate the Bacillus aureus strain in 100mL LB liquid medium, put it into a shaker for constant temperature cultivation (30°C, 160rpm), cultivate to the late logarithmic growth phase, and centrifuge the medium at room temperature (×4000g, 10min), Then washed with sterile water, resuspended in 0.9% physiological saline to obtain a bacterial suspension with an OD600 value of 0.85, then added 0.8 g of the biochar to 50 mL of the bacterial suspension, and let stand for ...

Embodiment 3

[0068] A preparation method of microbial immobilized composite particles:

[0069] S1. After washing and drying the rice bran with deionized water, put it in a crucible and place it in a tube furnace. Under an argon atmosphere, react at 650° C. for 4 h. After cooling to room temperature, take out the rice husk, and then use 0.1 mol· L -1 HCl and HNO 3 The mixed solution is washed until the lotion is neutral, the rice husks are dried, and then ground through a 100-mesh sieve to obtain biochar;

[0070] S2. Inoculate the Bacillus aureus strain in 100mL LB liquid medium, put it into a shaker for constant temperature cultivation (30°C, 160rpm), cultivate to the late logarithmic growth phase, and centrifuge the medium at room temperature (×4000g, 10min), Then washed with sterile water, resuspended in 0.9% physiological saline to obtain a bacterial suspension with an OD600 value of 1.0, then 1.0 g of the biochar was added to 50 mL of the bacterial suspension, and allowed to stand ...

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Abstract

The invention relates to the field of environmental bioremediation, in particular to a preparation method and application of microbial immobilized composite particles. The preparation method comprises the following steps: S1, washing, drying and heating a biomass material for reaction, washing the biomass material with inorganic strong acid, and then drying, grinding and sieving the biomass material to obtain biochar; s2, culturing a Chryseobacterium strain, centrifuging, washing, resuspending to obtain a bacterial suspension, and adding the charcoal to obtain a mixed solution; heating and dissolving polyvinyl alcohol and sodium alginate, sterilizing, and adding the mixed solution to obtain an embedding solution; dissolving calcium chloride and boric acid in water to obtain a crosslinking solution; s3, dropwise adding the embedding liquid into the cross-linking liquid, and washing after reaction to obtain the microbial immobilized composite particles. The prepared microbial immobilized composite particles have the advantages of good mass transfer performance, good mechanical performance, large specific surface area and the like, and have a good degradation effect on neonicotinoid pesticides in water.

Description

technical field [0001] The invention relates to the field of environmental bioremediation, in particular to a preparation method and application of a microorganism-immobilized composite particle. Background technique [0002] Neonicotinoids are systemic neuroactive insecticides. It kills insects by blocking the normal conduction of the central nervous system of insects. It is widely used in the control of insect pests such as vegetables and fruits, food crops and human settlements. It is the most widely used class of insecticides in the world. Neonicotinoids are persistent, migratory and bioaccumulative, and can cause extremely serious effects on the endocrine, respiratory, reproductive and nervous systems of non-target organisms (such as bees, birds, aquatic organisms and humans). Potentially carcinogenic, mutagenic and teratogenic. During use, neonicotinoid pesticides can enter the sewage treatment system through runoff rainwater and domestic sewage, or be directly disch...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/084C02F3/34C12R1/01C02F101/30
CPCC12N11/14C12N11/10C12N11/084C02F3/34C02F2101/306C02F2101/40Y02W10/10
Inventor 项雪珠黄明智易晓辉郑婉冰
Owner SOUTH CHINA NORMAL UNIVERSITY
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