Method of treating reclaimed water containing chryseobacterium meningosepticum

A golden bacterium and septic technology, which is applied in the field of inhibiting the infection risk of meningoseptica aureus in water, can solve the problems of population outbreak risk and substandard bacterial population suppression, and achieve the effect of reducing infection risk.

Active Publication Date: 2019-07-12
JILIN INST OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a method for treating reclaimed water containing Chryseobacterium meningosepticum, so as to solve the risk

Method used

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  • Method of treating reclaimed water containing chryseobacterium meningosepticum
  • Method of treating reclaimed water containing chryseobacterium meningosepticum
  • Method of treating reclaimed water containing chryseobacterium meningosepticum

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Include the following steps:

[0030] (a) Add bioflocculant solution to the regenerated water containing Chryseobacterium meningosepticum, stir evenly, the volume ratio of the regenerated water containing Chryseobacterium meningosepticum and bioflocculation agent solution is 90:5, the bioflocculant solution The concentration is 3g / ml;

[0031] (b) Transform the pNJR6 plasmid into Escherichia coli cells by heat shock transformation method or electroporation method, with a minimum transformation rate of 10%, as a lethal plasmid donor bacterium;

[0032] (c) Add 10 to the regenerated water of Chryseobacterium meningosepticum solution added to step (a) 3A / L lethal plasmid donor bacteria, and adding a concentration of 50mg / L nano-scale graphene oxide, the pH value is 7, after 24 hours, the lethality to Chryseobacterium meningosepticum is greater than 50%, and the lethality after 36 hours can be Up to 80% or more;

[0033] (d) After 60 hours, add a bioflocculant solution w...

Embodiment 2

[0035] Include the following steps:

[0036] (a) Add a bioflocculant solution to the regenerated water containing Chryseubacterium meningosepticum, stir evenly, the volume ratio of the regenerated water containing Chryseubacterium meningosepticum to the bioflocculant solution is 90:8, the bioflocculant solution The concentration is 4g / ml;

[0037] (b) Transform the pNJR6 plasmid into Escherichia coli cells by heat shock transformation method or electroporation method, with a minimum transformation rate of 10%, as a lethal plasmid donor bacterium;

[0038] (c) Add 10 to the regenerated water of Chryseobacterium meningosepticum solution added to step (a) 3 A / L lethal plasmid donor bacteria, and adding a concentration of 65mg / L nano-scale graphene oxide, the pH value is 7, after 24 hours, the lethality to Chryseobacterium meningosepticum is greater than 50%, and the lethality after 36 hours can be Up to 80% or more;

[0039] (d) After 60 hours, add a bioflocculant solution wit...

Embodiment 3

[0041] Include the following steps:

[0042] (a) Add bioflocculant solution to the regenerated water containing Chryseobacterium meningosepticum, stir evenly, the volume ratio of the regenerated water containing Chryseubacterium meningosepticum and bioflocculation agent solution is 90:10, the bioflocculant solution The concentration is 5g / ml;

[0043] (b) Transform the pNJR6 plasmid into Escherichia coli cells by heat shock transformation method or electroporation method, with a minimum transformation rate of 10%, as a lethal plasmid donor bacterium;

[0044] (c) Add 10 to the regenerated water of Chryseobacterium meningosepticum solution added to step (a) 3 A / L lethal plasmid donor bacteria, and adding a concentration of 80mg / L nano-scale graphene oxide, the pH value is 7, after 24 hours, the lethality to Chryseobacterium meningosepticum is greater than 50%, and the lethality after 36 hours can be Up to 80% or more;

[0045] (d) After 60 hours, add a bioflocculant solution...

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Abstract

The invention relates to a method of treating reclaimed water containing chryseobacterium meningosepticum and belongs to the field of water treatment. The method comprises the steps of adding a biological flocculant solution into the reclaimed water containing the chryseobacterium meningosepticum, and adding 103 lethal plasmid donor bacteria per L and nano graphite oxide with a concentration of 50-80mg/L. A lethality rate of the chryseobacterium meningosepticum can reach above 50% after 24h, and above 80% after 36h. The method has the advantages that a biological flocculant secreted from bacillus subtilis is used for efficient flocculation of the chryseobacterium meningosepticum and relevant florae, so that promotion of flora cell flocculation and polymerization is achieved; in addition, the transmission efficiency of pNJR6 plasmids in the florae in a chryseobacterium meningosepticum conjugation process is improved by low-concentration graphite oxide; and the method achieves the purpose of flocculation removal and induction killing to reduce an infection risk of the chryseobacterium meningosepticum in the reclaimed water.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to a method for inhibiting the infection risk of Chryseobacterium meningosepticum in water. Background technique [0002] Reclaimed water is the reuse of sewage after treatment, and is internationally recognized as the "second water source". In the utilization of urban reclaimed water, reclaimed water mainly refers to non-drinking water that can be reused within a certain range after urban sewage or domestic sewage has reached a certain water quality standard after treatment. According to relevant statistics, 80% of urban water supply is converted into sewage, and after collection and treatment, 70% of the reclaimed water can be recycled again. This means that through the reuse of sewage, the city's available water can be increased by at least 50% while the existing water supply remains unchanged. All countries in the world pay attention to the utilization of recycled wa...

Claims

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Application Information

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IPC IPC(8): C02F9/14
CPCC02F1/5263C02F1/56C02F3/34C02F2303/04
Inventor 徐亮孙彩云孙大志庄志军
Owner JILIN INST OF CHEM TECH
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