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Tumor cell membrane coated nanometer material, method for preparing same and application of tumor cell membrane coated nanometer material

A nanomaterial and tumor cell technology, applied in the field of glucose oxidase-loaded nanomaterials and their preparation, can solve problems such as limiting the drug delivery efficiency of tumor tissue, and achieve great clinical application potential, enhanced enrichment ability, and good biocompatibility. sexual effect

Active Publication Date: 2018-12-25
WUHAN UNIV
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  • Claims
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Problems solved by technology

The main reason is that the immune system can easily recognize nanoparticles as invaders, resulting in strong immune rejection, which limits the efficiency of drug delivery to tumor tissues.

Method used

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  • Tumor cell membrane coated nanometer material, method for preparing same and application of tumor cell membrane coated nanometer material
  • Tumor cell membrane coated nanometer material, method for preparing same and application of tumor cell membrane coated nanometer material
  • Tumor cell membrane coated nanometer material, method for preparing same and application of tumor cell membrane coated nanometer material

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Embodiment Construction

[0043] The features and advantages of the present invention can be further understood through the following detailed description in conjunction with the accompanying drawings. The examples provided are only illustrative of the method of the present invention and do not limit the remaining content of the present disclosure in any way. 1. Experimental part

[0044] 1. Materials and reagents

[0045] PBS (pH=7.4) and bovine embryo serum were purchased from Thermo Fisher Scientific (USA). Mesoporous silica nanoparticles were purchased from Shanghai Carboxyphenanthrene Biological Co., Ltd., with a diameter of 80-100 nm. EDTA, paraformaldehyde, DAPI, FITC, FDA, PI, CCK-8, BCA kit and phosphotungstic acid were purchased from Sigma (USA). SDS sample buffer and SDS gel were purchased from Beyond (China). DSPE-PEG-Cy5 was purchased from nanocs (USA). PD-1 immunosuppressant was purchased from ebioscience (USA). Other reagents were purchased from Sinopharm Chemical Reagent Co., Ltd....

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Abstract

The invention discloses a tumor cell membrane coated nanometer material, a method for preparing the same and application of the tumor cell membrane coated nanometer material, and belongs to the fieldof nanometer materials. The tumor cell membrane coated nanometer material is particularly a tumor site targeting bionic nanometer material. Cancer cell membranes are coated on the surfaces of mesoporous silicon dioxide nanometer particles (MSN), and glucose oxidase (GOx) is loaded on the MSN, so that hunger treatment can be carried out. The tumor cell membrane coated nanometer material, the methodand the application have the advantages that the surfaces of bionic materials are functionalized, CMSN-GOx has immune escape and homologous targeting capability, and accordingly enrichment of nanometer particles on tumor sites can be obviously improved; tumor can be partially ablated by the prepared CMSN-GOx nanometer particles, and certain antitumor immune response can be generated by means of tumor membrane induction; the tumor can be effectively ablated by the aid of hunger therapy by the CMSN-GOx combined with PD-1 immunotherapy as compared with injection of only PD-1 immunosupressant orthe CMSN-GOx, and adaptive immune response can be induced; the tumor cell membrane coated nanometer material is excellent in biocompatibility and has great clinical application potential.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a tumor cell membrane-coated nanomaterial loaded with glucose oxidase (hereinafter referred to as: CMSN-GOx) and its preparation method and application. Background technique [0002] In recent years, tumor immunology has attracted extensive attention due to its immunomodulatory means against tumors. Furthermore, the immune checkpoint inhibitor-programmed apoptosis protein 1 (PD-1) is a typical negative regulator of tumor-infiltrating lymphocytes (TILs). Although PD-1 immunotherapy has a good clinical therapeutic effect, the off-target effect is still obvious, and it will also bring some adverse reactions. In addition, the greater harm of these drugs is that they may cause autoimmune dysfunction. Therefore, how to improve the effect of cancer treatment and reduce off-target effects is the key factor of PD-1 immunotherapy. For example, checkpoint inhibition com...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K47/04A61K39/395A61P35/00A61K38/44
CPCA61K38/443A61K39/0011A61K39/39558A61K47/02A61P35/00C12Y101/03004A61K2300/00
Inventor 刘威谢伟陈贝朱道明吴文韬
Owner WUHAN UNIV
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