49results about How to "Great potential for clinical application" patented technology

The invention discloses a kit used for tumor exosome nanometer fluorescence detection and application of the kit. The kit comprises an identification element, a fluorescence reporter group and a fluorescence quenching group. The kit and the application thereof not only confirm that the PSMA on the human plasma source exosome can be used as a biomarker for identifying a health volunteer and a prostate cancer patient but further proves that the influence of normal cell source exosome in a clinical plasma specimen on a detection result can be effectively avoided through the method, and the kit has good anti-interference capability and a relatively large clinical application potential in the aspect of prostate cancer PSMA(+) exosome subgroup analysis.

The invention discloses a dual-ring hairpin probe mediate label-free strand displacement amplification method for detecting bleomycin. The method includes the steps that when bleomycin exists, a dual-ring hairpin probe fractures at a recognition site, and a triggering sequence is released; the triggering sequence and a ring part of a signal probe are combined and subjected to a strand displacement amplification reaction under the effect of a polymerase and a nicking enzyme, and finally a great number of G-four-chain sequences are generated. At the same time, a primer chain extends to open the signal probe, and therefore the G-four-chain sequences packaged in the neck of the signal probe can be exposed. Finally, NMM molecules are bound to the G-four-chain sequences to generate fluorescence signals, and bleomycin is quantified through the detected fluorescence signals. As the triggering sequence is designed at the neck of the dual-ring hairpin probe, background signals of a detection system are reduced; by combining bleomycin cutting and the strand displacement amplification reaction, bleomycin can be detected sensitively, and the detection limit is 0.34 nm. The method has the advantages of being easy to operate, free of labeling and good in specificity.

The invention discloses a method and a system for automatically segmenting an esophageal cancer radiotherapy target area and organs at risk, and relates to the field of medical image segmentation. Themethod comprises the following steps: extracting features of an input CT image through a residual network, and fusing multi-scale feature maps through a feature pyramid network; screening the regionof interest of each point in the feature map through a region suggestion network; pooling the region-of-interest screened by the region suggestion network to a fixed size in combination with the region-of-interest alignment layer; inputting a region of interest pooled to a fixed size into the full connection layer for organ classification, and performing organ position border regression; meanwhile, inputting the interest area pooled to a fixed size into the organ segmentation network. The method has the advantage that the accuracy of automatically segmenting the esophagus cancer radiotherapy target area and various organs at risk is improved.

The invention provides chitosan-modified methazolamide solid lipid nanoparticles and a preparation method thereof. The chitosan-modified methazolamide solid lipid nanoparticles can be used as eye drops. The preparation method is suitable for industrial production. The chitosan-modified methazolamide solid lipid nanoparticles are characterized in that based on 30ml of a nanoparticle-containing solution, the chitosan-modified methazolamide solid lipid nanoparticles comprise 5mg of methazolamide, 25 to 150mg of at least one lipid material, 25 to 150mg of phospholipid, 5 to 100mg of chitosan, 50 to 250mg of at least one non-phospholipid surfactant and 50 to 250mg of at least one assistant surfactant. The chitosan-modified methazolamide solid lipid nanoparticles have small particle sizes and high drug entrapment efficiency. Compared with solid lipid nanoparticles which are not modified by chitosan, the chitosan-modified methazolamide solid lipid nanoparticles have higher stability and better corneal permeability because of positive charges on the surfaces of the chitosan-modified methazolamide solid lipid nanoparticles so that drug bioavailability is improved. Therefore, the chitosan-modified methazolamide solid lipid nanoparticles have a large clinical application potential in glaucoma treatment.

The invention discloses a method for using a Pickering emulsion method for producing GelMA macropore hydrogel and application. The method comprises the steps of dissolving GelMA and PEG-4SH in PBS buffer liquid, and stirring a mixture; adding MgO nano-particles after fully dissolving the GelMA and the PEG-4SH, stirring a mixture, and conducting ultrasonic treatment on the mixture; adding dodecaneand a photoinitiator, and using a high-speed homogenizer for intensively stirring a mixture to obtain stable Pickering emulsion; and making the emulsion subjected to UV illumination to form gel, and obtaining the GelMA macropore hydrogel by using ethyl alcohol and water for full wash dialysis. The produced GelMA macropore hydrogel has the advantage of the macropore structure, can promote transportation of nutrient substances, discharging of metabolic products and cell communication, entraps a component containing Mg, has effect of promoting adhesion and proliferation of bone marrow mesenchymalstem cells and promoting osteogenic differentiation, has excellent biocompatibility, can be used as a bone tissue engineering scaffold with excellent performance, and has large clinical application potential in the field of bone repair.

The invention discloses a DNA mutation test method in use of nano-Au, relates to a DNA mutation test method, and provides a DNA mutation test method that uses a nano-Au probe. The DNA mutation test method leads wild single-stranded DNA, a probe that is completely complementary with a wild single-stranded DNA sequence and the nano-Au to be mixed, statically placed, added with a sodium chloride solution, and then statically placed, thus obtaining an architecture 1; the DNA mutation test method further leads mutant single-stranded DNA, the probe that is completely complementary with the wild single-stranded DNA sequence and the nano-Au to be mixed, statically placed, added with the sodium chloride solution, and then statically placed, thus obtaining an architecture 2; the sodium chloride solution is added into and evenly mixed with the architecture 1, the ultraviolet-visible absorption spectrum detection is implemented, and an absorbance value that is obtained at a 520nm position is taken as an absorbance value 1; the sodium chloride solution is added into and evenly mixed with the architecture 2, the ultraviolet-visible absorption spectrum detection is implemented, and an absorbance value that is obtained at a 520nm position is taken as an absorbance value 2; when the absorbance values 2 and 1 have marked difference, mutation is determined to exist. The DNA mutation test method does not need any modification of the DNA and the nano-Au, thus simplifying sample processing.

The invention discloses a branched polyetherimide material containing a ketone thioacetal bond, as well as a preparation method and application thereof. The preparation method comprises the followingsteps: (1) stirring acetone and acid containing thiol to react at room temperature; (2) adding polyetherimide and an activator after the reaction is finished, dissolving in an organic reagent, stirring to react, bonding chlorin e6 and carboxyl polyethylene glycol through an amidation reaction to obtain the reactive oxygen sensitive branched polyetherimide material containing the ketone thioacetalbond. The branched polyetherimide material has excellent biocompatibility and degradability, is entrapped with siRNA, and forms nano-particles by self-assembly, can generate a great number of reactiveoxygen under excitation of a light source with specific wavelength to destroy the structure of endosome, the reactive oxygen sensitive ketone thioacetal bond is broken, particles are disintegrated, and endosome escape and release of intracellular siRNA can be accelerated. The branched polyetherimide material containing a ketone thioacetal bond has a huge clinical application potential in the field of tumor treatment.

The invention relates to the technical field of genetic engineering and biological agents, and particularly discloses octanoic acid acylation modified antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO. 1. The octanoic acid acylation modified antibacterial peptide simulates the arrangement rule of alpha-spiral secondary structure amino acid in natural protein, leucine and arginine provide hydrophobicity and cationicity respectively, a heptapeptide repetitive sequence 'abcdefg' is used as a template, the sequence is repeated twice, then the tail end of the sequence N is subjected to octanoic acid acylation modification, and the tail end of the sequence C is amidated. The peptide has broad-spectrum antibacterial activity on fungi and bacteria and has wide clinical application potential.

The invention relates a method for inducing in-vitro expansion of CD8<+> regulatory T cells by immunosuppressants. According to the method, expansion of CD8<+> regulatory T cells is jointly induced by TGF-beta and RAPA (rapamycin). The invention further provides a CD8<+> Treg (regulatory T cell) with an immunosuppression function and application of the CD8<+> Treg with the immunosuppression function. The method has the advantages that the TGF-beta and the RAPA are added into a polycloning in-vitro expansion system of the CD8<+> regulatory T cells for the first time, and a great number of CD8<+> Tregs with the remarkable immunosuppression function are obtained successfully; as multi-round expansion is conducted, the CD8<+> Tregs can increase exponentially so as to meet the demands of clinical cell treatment. The CD8<+> Tregs can be kept stable in the aspects of activity, purity, phenotype, nullipotency, suppression function and the like and can be used for treating various autoimmune diseases through adoptive infusion, thereby being huge in clinical application potential.

The invention discloses a multi-crosslinked high-strength enzyme-induced mineralized hydrogel and a preparation method and application thereof. The preparation method comprises the following steps of (1) adding sodium alginate and acrylamide into deionized water, and heating to completely dissolve the sodium alginate to obtain a solution A, (2) mixing an alkaline phosphatase aqueous solution and the solution A, stirring, adding a methylene bisacrylamide aqueous solution and an accelerator, continuously stirring, adding an initiator, continuously stirring, finally degassing, transferring into a mold, and drying under a closed condition to form solid hydrogel, and (3) soaking the solid hydrogel in an ionic solution overnight, then taking out the solid hydrogel, soaking the solid hydrogel in a calcium glycerophosphate mineralizing solution, and mineralizing the solid hydrogel in a dark condition to prepare the multi-crosslinked high-strength enzyme-induced mineralized hydrogel. The hydrogel material prepared by the invention is good in toughness and excellent in mechanical strength, and has relatively good osteogenesis biological activity.

The invention relates to a protein type nanoparticle for delivery of multiple specific antibodies, and application and a preparation method of the protein type nanoparticle. The protein type nanoparticle comprises polyester and protein with a hydrophobic structural domain; the hydrophobic structural domain of the protein is combined with the polyester through hydrophobic interaction; and the protein is at least one of albumin, globulin and cell wall protein. The protein type nanoparticle disclosed by the invention has excellent stability and biocompatibility, and can be used for preparing a multi-specific antibody delivery platform (alphaFc-NP) by bonding an anti-IgG-Fc antibody or an anti-IgG-Fc antibody fragment; the alphaFc-NP can be stably, rapidly, simply and conveniently combined with multiple specific antibodies through antigen-antibody interaction force; and furthermore, the treatment effect of the specific antibodies is enhanced.

The invention discloses high dietary fiber vine tea green juice powder and a preparation method thereof. The powder is prepared from the following raw materials in parts by weight: 8-10 parts of vinetea, 5-7 parts of strigose hydrangea juvenile leaves, 3-5 parts of mulberry leaves, 3-5 parts of chlorella, 1-3 parts of burdock, 1-2 parts of lotus leaves, 1-2 parts of sugar alcohol and 1-2 parts ofbamboo salt. The preparation method comprises the following steps: pre-treating the raw materials, performing secondary squeezing for the vine tea, strigose hydrangea juvenile leaves, mulberry leaves, chlorella and lotus leaves to obtain green juice, and performing drying and grinding to obtain a crude product green juice powder; directly drying and grinding the burdock to obtain burdock powder,mixing the burdock powder with the crude product green juice to obtain green juice powder, adding the bamboo salt and sugar alcohol into the green juice powder, performing uniform mixing and sterilizing to obtain the high dietary fiber vine tea green juice powder. The preparation method is simple, the green juice powder is rich in flavones, rubusosides and other active ingredients having remarkable effects for reducing blood glucose, blood pressure and blood fat, has rich dietary fibers, and is an ideal solid drink for the group with hypertension, hyperglycemia and hyperlipidemia.

The invention discloses a high-dietary fiber ampelopsis grossedentata green juice powder and a preparation method thereof. The raw materials are as follows: 8-10 parts of ampelopsis grossedentata, 5-7parts of leaves of strigose hydrangea, 3-5 parts of mulberry leaves, 3-5 parts of chlorella, 1-3 parts of great burdock achene, 1-2 parts of lotus leaves, 1-2 parts of sugar alcohol, and 1-2 parts ofbamboo salt. The preparation method comprises the following steps: first pretreating raw materials, then subjecting the ampelopsis grossedentata, the leaves of strigose hydrangea, the mulberry leaves, the chlorella and the lotus leaves to secondary juicing to obtain green juice, and performing drying and pulverizing to obtain a crude green juice powder; and directly drying and crushing the greatburdock achene to obtain a great burdock achene powder, mixing the great burdock achene powder with the crude green juice powder, then adding the bamboo salt and the sugar alcohol, and performing uniform mixing and sterilization to obtain the high-dietary fiber ampelopsis grossedentata green juice powder. The preparation method of the invention is simple, and the prepared green juice powder not only contains rich in active ingredients such as flavonoids and catechins which have significant effects on lowering blood sugar, lowering blood pressure and lowering blood fat, but also contains rich dietary fibers, and is an ideal solid drink for people with three highs (high blood pressure, high blood fat and high blood sugar).

The invention discloses an MMT (montmorillonite)-Gd-DTPA (diethylenetriaminepentaacetic acid) compound, a synthetic method thereof and an application of the MMT-Gd-DTPA compound to magnetic resonance diagnosis for a digestive tract. The synthetic method is characterized in that MMT powder and GdCl3 are mixed in water and react, MMT-Gd compound powder is obtained and reacts with DTPA in water, and the MMT-Gd-DTPA compound is obtained. The MMT-Gd-DTPA compound is obtained through hydro-thermal synthesis, the method is simple and easy to implement, the obtained compound not only has good longitudinal relaxation rate r1 and good biocompatibility, but also has good effect of adhesion to the digestive tract wall, is a better T1 magnetic resonance molecular imaging oral contrast agent and has higher clinical application potential.

The invention belongs to the technical field of collagen biomimetic materials, and particularly relates to a covalent photo-crosslinking polypeptide, and a collagen biomimetic material formed by self-assembly of the covalent photo-crosslinking polypeptide. The covalent photo-crosslinking polypeptide is a polypeptide sequence capable of forming a triple helix structure, and comprises T1 and T2 structural domains with continuous Tyr at two ends, N1 and N2 structural domains with repeated Gly-Xaa-Yaa and/or Gly-Xaa-Xaa, and an M structural domain containing Tyr between the N1 and N2 structural domains. The covalent photo-crosslinking polypeptide is self-assembled under photocatalysis to form a biomimetic material capable of simulating the structure and function of natural collagen; a polypeptide fragment with a biological function can be conveniently introduced into the M structural domain, self-assembly is not influenced, and the polypeptide self-assembly strategy has wide applicability; and the prepared biomimetic material has good biocompatibility, can be used as a scaffold material for cell growth, and has wide application prospects in the fields of tissue engineering and regenerative medicine.