49results about How to "Great potential for clinical application" patented technology

The invention discloses a kit used for tumor exosome nanometer fluorescence detection and application of the kit. The kit comprises an identification element, a fluorescence reporter group and a fluorescence quenching group. The kit and the application thereof not only confirm that the PSMA on the human plasma source exosome can be used as a biomarker for identifying a health volunteer and a prostate cancer patient but further proves that the influence of normal cell source exosome in a clinical plasma specimen on a detection result can be effectively avoided through the method, and the kit has good anti-interference capability and a relatively large clinical application potential in the aspect of prostate cancer PSMA(+) exosome subgroup analysis.

The invention discloses a dual-ring hairpin probe mediate label-free strand displacement amplification method for detecting bleomycin. The method includes the steps that when bleomycin exists, a dual-ring hairpin probe fractures at a recognition site, and a triggering sequence is released; the triggering sequence and a ring part of a signal probe are combined and subjected to a strand displacement amplification reaction under the effect of a polymerase and a nicking enzyme, and finally a great number of G-four-chain sequences are generated. At the same time, a primer chain extends to open the signal probe, and therefore the G-four-chain sequences packaged in the neck of the signal probe can be exposed. Finally, NMM molecules are bound to the G-four-chain sequences to generate fluorescence signals, and bleomycin is quantified through the detected fluorescence signals. As the triggering sequence is designed at the neck of the dual-ring hairpin probe, background signals of a detection system are reduced; by combining bleomycin cutting and the strand displacement amplification reaction, bleomycin can be detected sensitively, and the detection limit is 0.34 nm. The method has the advantages of being easy to operate, free of labeling and good in specificity.

The invention discloses a method and a system for automatically segmenting an esophageal cancer radiotherapy target area and organs at risk, and relates to the field of medical image segmentation. Themethod comprises the following steps: extracting features of an input CT image through a residual network, and fusing multi-scale feature maps through a feature pyramid network; screening the regionof interest of each point in the feature map through a region suggestion network; pooling the region-of-interest screened by the region suggestion network to a fixed size in combination with the region-of-interest alignment layer; inputting a region of interest pooled to a fixed size into the full connection layer for organ classification, and performing organ position border regression; meanwhile, inputting the interest area pooled to a fixed size into the organ segmentation network. The method has the advantage that the accuracy of automatically segmenting the esophagus cancer radiotherapy target area and various organs at risk is improved.

The invention provides chitosan-modified methazolamide solid lipid nanoparticles and a preparation method thereof. The chitosan-modified methazolamide solid lipid nanoparticles can be used as eye drops. The preparation method is suitable for industrial production. The chitosan-modified methazolamide solid lipid nanoparticles are characterized in that based on 30ml of a nanoparticle-containing solution, the chitosan-modified methazolamide solid lipid nanoparticles comprise 5mg of methazolamide, 25 to 150mg of at least one lipid material, 25 to 150mg of phospholipid, 5 to 100mg of chitosan, 50 to 250mg of at least one non-phospholipid surfactant and 50 to 250mg of at least one assistant surfactant. The chitosan-modified methazolamide solid lipid nanoparticles have small particle sizes and high drug entrapment efficiency. Compared with solid lipid nanoparticles which are not modified by chitosan, the chitosan-modified methazolamide solid lipid nanoparticles have higher stability and better corneal permeability because of positive charges on the surfaces of the chitosan-modified methazolamide solid lipid nanoparticles so that drug bioavailability is improved. Therefore, the chitosan-modified methazolamide solid lipid nanoparticles have a large clinical application potential in glaucoma treatment.

The invention discloses a method for using a Pickering emulsion method for producing GelMA macropore hydrogel and application. The method comprises the steps of dissolving GelMA and PEG-4SH in PBS buffer liquid, and stirring a mixture; adding MgO nano-particles after fully dissolving the GelMA and the PEG-4SH, stirring a mixture, and conducting ultrasonic treatment on the mixture; adding dodecaneand a photoinitiator, and using a high-speed homogenizer for intensively stirring a mixture to obtain stable Pickering emulsion; and making the emulsion subjected to UV illumination to form gel, and obtaining the GelMA macropore hydrogel by using ethyl alcohol and water for full wash dialysis. The produced GelMA macropore hydrogel has the advantage of the macropore structure, can promote transportation of nutrient substances, discharging of metabolic products and cell communication, entraps a component containing Mg, has effect of promoting adhesion and proliferation of bone marrow mesenchymalstem cells and promoting osteogenic differentiation, has excellent biocompatibility, can be used as a bone tissue engineering scaffold with excellent performance, and has large clinical application potential in the field of bone repair.

The invention belongs to the field of medicine preparations and particularly relates to a natural nanoparticle-medicine composition for resisting Alzheimer's disease (AD) and a preparation method, property evaluation and application thereof. By recombining extracted natural lipoprotein nanoparticles and AD treatment medicine to prepare the natural nanoparticle-medicine composition, combined treatment of AD is achieved. The combined treatment is high in homologous bionic performance and safety, high in medicine loading capacity, efficient in brain targeting, high in amyloid protein affinity, capable of achieving targeting removing, mild in preparation conditions, simple in preparation process and easy in industry expanding. The natural nanoparticle-medicine composition is administrated through intravenous injection, oral taking, nasal delivery and the like, a new thought and technology is provided for the research and development of new AD medicine, and the natural nanoparticle-medicinecomposition has an important research value and promising clinical research prospect.

The invention discloses a DNA mutation test method in use of nano-Au, relates to a DNA mutation test method, and provides a DNA mutation test method that uses a nano-Au probe. The DNA mutation test method leads wild single-stranded DNA, a probe that is completely complementary with a wild single-stranded DNA sequence and the nano-Au to be mixed, statically placed, added with a sodium chloride solution, and then statically placed, thus obtaining an architecture 1; the DNA mutation test method further leads mutant single-stranded DNA, the probe that is completely complementary with the wild single-stranded DNA sequence and the nano-Au to be mixed, statically placed, added with the sodium chloride solution, and then statically placed, thus obtaining an architecture 2; the sodium chloride solution is added into and evenly mixed with the architecture 1, the ultraviolet-visible absorption spectrum detection is implemented, and an absorbance value that is obtained at a 520nm position is taken as an absorbance value 1; the sodium chloride solution is added into and evenly mixed with the architecture 2, the ultraviolet-visible absorption spectrum detection is implemented, and an absorbance value that is obtained at a 520nm position is taken as an absorbance value 2; when the absorbance values 2 and 1 have marked difference, mutation is determined to exist. The DNA mutation test method does not need any modification of the DNA and the nano-Au, thus simplifying sample processing.

The invention discloses a branched polyetherimide material containing a ketone thioacetal bond, as well as a preparation method and application thereof. The preparation method comprises the followingsteps: (1) stirring acetone and acid containing thiol to react at room temperature; (2) adding polyetherimide and an activator after the reaction is finished, dissolving in an organic reagent, stirring to react, bonding chlorin e6 and carboxyl polyethylene glycol through an amidation reaction to obtain the reactive oxygen sensitive branched polyetherimide material containing the ketone thioacetalbond. The branched polyetherimide material has excellent biocompatibility and degradability, is entrapped with siRNA, and forms nano-particles by self-assembly, can generate a great number of reactiveoxygen under excitation of a light source with specific wavelength to destroy the structure of endosome, the reactive oxygen sensitive ketone thioacetal bond is broken, particles are disintegrated, and endosome escape and release of intracellular siRNA can be accelerated. The branched polyetherimide material containing a ketone thioacetal bond has a huge clinical application potential in the field of tumor treatment.

The invention relates to the technical field of genetic engineering and biological agents, and particularly discloses octanoic acid acylation modified antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO. 1. The octanoic acid acylation modified antibacterial peptide simulates the arrangement rule of alpha-spiral secondary structure amino acid in natural protein, leucine and arginine provide hydrophobicity and cationicity respectively, a heptapeptide repetitive sequence 'abcdefg' is used as a template, the sequence is repeated twice, then the tail end of the sequence N is subjected to octanoic acid acylation modification, and the tail end of the sequence C is amidated. The peptide has broad-spectrum antibacterial activity on fungi and bacteria and has wide clinical application potential.

The invention relates a method for inducing in-vitro expansion of CD8<+> regulatory T cells by immunosuppressants. According to the method, expansion of CD8<+> regulatory T cells is jointly induced by TGF-beta and RAPA (rapamycin). The invention further provides a CD8<+> Treg (regulatory T cell) with an immunosuppression function and application of the CD8<+> Treg with the immunosuppression function. The method has the advantages that the TGF-beta and the RAPA are added into a polycloning in-vitro expansion system of the CD8<+> regulatory T cells for the first time, and a great number of CD8<+> Tregs with the remarkable immunosuppression function are obtained successfully; as multi-round expansion is conducted, the CD8<+> Tregs can increase exponentially so as to meet the demands of clinical cell treatment. The CD8<+> Tregs can be kept stable in the aspects of activity, purity, phenotype, nullipotency, suppression function and the like and can be used for treating various autoimmune diseases through adoptive infusion, thereby being huge in clinical application potential.

The invention discloses a sodium alginate / silver loaded graphene composite film having bacterium resistance and wound healing promotion functions and application thereof. The composite film is characterized by being prepared through the following steps: uniformly mixing a silver loaded graphene solution and a sodium alginate solution, spraying and assembling. The composite film provided by the invention is simple in preparation method, simultaneously has bacterium resistance and wound healing promotion functions, and achieves an obvious effect when being used as a wound dressing, thereby having great clinical application potentials.

The invention discloses a multi-crosslinked high-strength enzyme-induced mineralized hydrogel and a preparation method and application thereof. The preparation method comprises the following steps of (1) adding sodium alginate and acrylamide into deionized water, and heating to completely dissolve the sodium alginate to obtain a solution A, (2) mixing an alkaline phosphatase aqueous solution and the solution A, stirring, adding a methylene bisacrylamide aqueous solution and an accelerator, continuously stirring, adding an initiator, continuously stirring, finally degassing, transferring into a mold, and drying under a closed condition to form solid hydrogel, and (3) soaking the solid hydrogel in an ionic solution overnight, then taking out the solid hydrogel, soaking the solid hydrogel in a calcium glycerophosphate mineralizing solution, and mineralizing the solid hydrogel in a dark condition to prepare the multi-crosslinked high-strength enzyme-induced mineralized hydrogel. The hydrogel material prepared by the invention is good in toughness and excellent in mechanical strength, and has relatively good osteogenesis biological activity.

The invention discloses a thioketal bond connected pegylated Ce6 material as well as a preparation method and application thereof. The method comprises the following steps: saturating a mixture of cysteamine hydrochloride and acetone with hydrogen chloride, and performing reaction to obtain diamine containing thioketal bonds; performing reaction on methoxy polyethylene glycol carboxyl, dicyclohexylcarbodiimide and N-hydroxysuccinimide, and dropwise adding a polyethylene glycol solution into a diamine solution containing thioketal for reaction to obtain mPEG-TK-NH2; dissolving chlorin e6, dicyclohexylcarbodiimide and N-hydroxysuccinimide in a solvent for reaction; and adding the activated Ce6 solution into the mPEG-TK-NH2 solution, and carrying out a stirring reaction to obtain the thioketal bond connected pegylated Ce6 material. The thioketal bond connected pegylated Ce6 material has biocompatibility and degradability, and can enhance the uptake of drug-loaded particles by cells, so that the drug concentration in the cells is improved, tumor cells are killed, and the chemotherapy effect is improved.

The invention relates to a protein type nanoparticle for delivery of multiple specific antibodies, and application and a preparation method of the protein type nanoparticle. The protein type nanoparticle comprises polyester and protein with a hydrophobic structural domain; the hydrophobic structural domain of the protein is combined with the polyester through hydrophobic interaction; and the protein is at least one of albumin, globulin and cell wall protein. The protein type nanoparticle disclosed by the invention has excellent stability and biocompatibility, and can be used for preparing a multi-specific antibody delivery platform (alphaFc-NP) by bonding an anti-IgG-Fc antibody or an anti-IgG-Fc antibody fragment; the alphaFc-NP can be stably, rapidly, simply and conveniently combined with multiple specific antibodies through antigen-antibody interaction force; and furthermore, the treatment effect of the specific antibodies is enhanced.

The invention discloses acinetobacter baumannii bacteriophage SH-Ab15519 and application thereof. Acinetobacter baumannii bacteriophage SH-Ab15519 is assigned the accession number CCTCC NO:M 2016653. The novel acinetobacter baumannii bacteriophage SH-Ab15519 is obtained by separation and purification and can peculiarly and effectively kill acinetobacter baumannii, have good in vivo and in vitro antibacterial effects on medicine-resistant acinetobacter baumannii, provide an experimental basis for clinically developing a preparation for preventing and treating infection by medicine-resistant acinetobacter baumannii and have great clinical application potential.

The invention discloses high dietary fiber vine tea green juice powder and a preparation method thereof. The powder is prepared from the following raw materials in parts by weight: 8-10 parts of vinetea, 5-7 parts of strigose hydrangea juvenile leaves, 3-5 parts of mulberry leaves, 3-5 parts of chlorella, 1-3 parts of burdock, 1-2 parts of lotus leaves, 1-2 parts of sugar alcohol and 1-2 parts ofbamboo salt. The preparation method comprises the following steps: pre-treating the raw materials, performing secondary squeezing for the vine tea, strigose hydrangea juvenile leaves, mulberry leaves, chlorella and lotus leaves to obtain green juice, and performing drying and grinding to obtain a crude product green juice powder; directly drying and grinding the burdock to obtain burdock powder,mixing the burdock powder with the crude product green juice to obtain green juice powder, adding the bamboo salt and sugar alcohol into the green juice powder, performing uniform mixing and sterilizing to obtain the high dietary fiber vine tea green juice powder. The preparation method is simple, the green juice powder is rich in flavones, rubusosides and other active ingredients having remarkable effects for reducing blood glucose, blood pressure and blood fat, has rich dietary fibers, and is an ideal solid drink for the group with hypertension, hyperglycemia and hyperlipidemia.

The invention discloses a double-locking polymer and a preparation method and application thereof. The structural formula of the double-lock polymer is as shown in the specification; in the formula, xis a natural number ranging from 110 to 117, y is a natural number ranging from 70 to 80, and z is a natural number ranging from 1 to 3. The double-locking polymer has good biocompatibility and degradability, and nanoparticles formed by self-assembly of the double-locking polymer can be used for tumor imaging and treatment.

The invention discloses an HPPE (hyperbranched polyphosphate ester) material of an acetal bond skeleton as well as a preparation method and an application of the HPPE material. The method comprises steps as follows: (1), diol containing acetal bonds and triethylamine are dissolved in a solvent, phosphorus oxychloride is added under the condition of ice bath, and stirring reaction is performed; (2), after the reaction, methoxy polyethylene glycol is added, the stirring reaction is performed, and the HPPE material of the acetal bond skeleton is obtained. The HPPE material has good biocompatibility and biodegradability; the HPPE material is self-assembled to form nanoparticles, each acetal bond is broken into two hydroxyls in the endosome / lysosome acid environment in tumor cells, phosphate ofparticle kernels is transformed from hydrophobicity to hydrophility, the particles are disintegrated, a photosensitizer drug is rapidly released, concentration of a free photosensitizer in the tumorcells is increased, a large amount of active oxygen is produced under excitation of a light source with specific wavelength kills the tumor cells, PDT curative effect is improved, and the HPPE material has great clinical application potential.

The invention relates to a cabazitaxel weakly-alkaline derivative and a preparation thereof, in particular to synthesis of the cabazitaxel weakly-alkaline derivative, a liposome preparation containingthe cabazitaxel weakly-alkaline derivative and application of the cabazitaxel weakly-alkaline derivative in a drug delivery system, and belongs to the technical field of medicines. According to the cabazitaxel weakly-alkaline derivative, cabazitaxel is connected with a weakly-alkaline intermediate through an ester bond, the ester bond can be broken under the action of esterase in vivo, and an active drug is released. The structural general formula is shown in the specification, wherein a connecting group is C1-C4 alkyl, C3-C6 naphthenic base or phenyl; [N] is an N-methyl piperazinyl group, apiperidinyl group, a 4-(1-piperidinyl) piperidinyl group, a morpholinyl group, a pyrrolidine group or other tertiary amine structures. The cabazitaxel weakly-alkaline derivative disclosed by the invention can be prepared into the liposome preparation. The liposome preparation has the characteristics of high drug loading capacity, high encapsulation efficiency, good stability and the like. After injection administration, the in-vivo circulation time of the drug can be greatly prolonged, the accumulation amount of the drug at a tumor part is increased, and the anti-tumor effect and the tolerancedose are improved. .

The invention discloses a high-dietary fiber ampelopsis grossedentata green juice powder and a preparation method thereof. The raw materials are as follows: 8-10 parts of ampelopsis grossedentata, 5-7parts of leaves of strigose hydrangea, 3-5 parts of mulberry leaves, 3-5 parts of chlorella, 1-3 parts of great burdock achene, 1-2 parts of lotus leaves, 1-2 parts of sugar alcohol, and 1-2 parts ofbamboo salt. The preparation method comprises the following steps: first pretreating raw materials, then subjecting the ampelopsis grossedentata, the leaves of strigose hydrangea, the mulberry leaves, the chlorella and the lotus leaves to secondary juicing to obtain green juice, and performing drying and pulverizing to obtain a crude green juice powder; and directly drying and crushing the greatburdock achene to obtain a great burdock achene powder, mixing the great burdock achene powder with the crude green juice powder, then adding the bamboo salt and the sugar alcohol, and performing uniform mixing and sterilization to obtain the high-dietary fiber ampelopsis grossedentata green juice powder. The preparation method of the invention is simple, and the prepared green juice powder not only contains rich in active ingredients such as flavonoids and catechins which have significant effects on lowering blood sugar, lowering blood pressure and lowering blood fat, but also contains rich dietary fibers, and is an ideal solid drink for people with three highs (high blood pressure, high blood fat and high blood sugar).

The invention discloses an MMT (montmorillonite)-Gd-DTPA (diethylenetriaminepentaacetic acid) compound, a synthetic method thereof and an application of the MMT-Gd-DTPA compound to magnetic resonance diagnosis for a digestive tract. The synthetic method is characterized in that MMT powder and GdCl3 are mixed in water and react, MMT-Gd compound powder is obtained and reacts with DTPA in water, and the MMT-Gd-DTPA compound is obtained. The MMT-Gd-DTPA compound is obtained through hydro-thermal synthesis, the method is simple and easy to implement, the obtained compound not only has good longitudinal relaxation rate r1 and good biocompatibility, but also has good effect of adhesion to the digestive tract wall, is a better T1 magnetic resonance molecular imaging oral contrast agent and has higher clinical application potential.

The invention belongs to the technical field of collagen biomimetic materials, and particularly relates to a covalent photo-crosslinking polypeptide, and a collagen biomimetic material formed by self-assembly of the covalent photo-crosslinking polypeptide. The covalent photo-crosslinking polypeptide is a polypeptide sequence capable of forming a triple helix structure, and comprises T1 and T2 structural domains with continuous Tyr at two ends, N1 and N2 structural domains with repeated Gly-Xaa-Yaa and / or Gly-Xaa-Xaa, and an M structural domain containing Tyr between the N1 and N2 structural domains. The covalent photo-crosslinking polypeptide is self-assembled under photocatalysis to form a biomimetic material capable of simulating the structure and function of natural collagen; a polypeptide fragment with a biological function can be conveniently introduced into the M structural domain, self-assembly is not influenced, and the polypeptide self-assembly strategy has wide applicability; and the prepared biomimetic material has good biocompatibility, can be used as a scaffold material for cell growth, and has wide application prospects in the fields of tissue engineering and regenerative medicine.

The invention discloses a preparation method and application of a heat-induced irreversible composite hydrogel with antibacterial and wound healing functions. After the gel is dissolved in a silver-loaded graphene solution, it is sprayed on the wound surface and stimulated by the temperature of the wound surface. An irreversible hydrogel is formed in response. The temperature-sensitive hydrogel provided by the present invention has a simple preparation method, and has the functions of antibacterial and wound healing at the same time. It is used as a wound dressing, has obvious effects, and has great potential for clinical application.

The invention discloses a method for using a Pickering emulsion method for producing GelMA macropore hydrogel and application. The method comprises the steps of dissolving GelMA and PEG-4SH in PBS buffer liquid, and stirring a mixture; adding MgO nano-particles after fully dissolving the GelMA and the PEG-4SH, stirring a mixture, and conducting ultrasonic treatment on the mixture; adding dodecaneand a photoinitiator, and using a high-speed homogenizer for intensively stirring a mixture to obtain stable Pickering emulsion; and making the emulsion subjected to UV illumination to form gel, and obtaining the GelMA macropore hydrogel by using ethyl alcohol and water for full wash dialysis. The produced GelMA macropore hydrogel has the advantage of the macropore structure, can promote transportation of nutrient substances, discharging of metabolic products and cell communication, entraps a component containing Mg, has effect of promoting adhesion and proliferation of bone marrow mesenchymalstem cells and promoting osteogenic differentiation, has excellent biocompatibility, can be used as a bone tissue engineering scaffold with excellent performance, and has large clinical application potential in the field of bone repair.

The invention discloses a DNA mutation test method in use of nano-Au, relates to a DNA mutation test method, and provides a DNA mutation test method that uses a nano-Au probe. The DNA mutation test method leads wild single-stranded DNA, a probe that is completely complementary with a wild single-stranded DNA sequence and the nano-Au to be mixed, statically placed, added with a sodium chloride solution, and then statically placed, thus obtaining an architecture 1; the DNA mutation test method further leads mutant single-stranded DNA, the probe that is completely complementary with the wild single-stranded DNA sequence and the nano-Au to be mixed, statically placed, added with the sodium chloride solution, and then statically placed, thus obtaining an architecture 2; the sodium chloride solution is added into and evenly mixed with the architecture 1, the ultraviolet-visible absorption spectrum detection is implemented, and an absorbance value obtained at a 520nm position is taken as an absorbance value 1; the sodium chloride solution is added into and evenly mixed with the architecture 2, the ultraviolet-visible absorption spectrum detection is implemented, and an absorbance value obtained at a 520nm position is taken as an absorbance value 2; when the absorbance values 2 and 1 have marked difference, mutation is determined to exist. The DNA mutation test method does not need any modification of the DNA and the nano-Au, thus simplifying sample processing.

The present invention relates to weakly basic derivatives of cabazitaxel and preparations thereof, in particular to the synthesis of weakly basic derivatives of cabazitaxel, liposome preparations containing the derivatives and their application in drug delivery systems, belonging to medical technology field. The weakly basic derivative of cabazitaxel is connected with a weakly basic intermediate through an ester bond, and the ester bond can be broken by the action of esterase in the body to release the active drug. Its structural general formula is as follows: wherein, linking group is C 1 -C 4 Alkyl, C 3 -C 6 Cycloalkyl or phenyl; [N] is N-methylpiperazinyl, piperidinyl, 4-(1-piperidinyl) piperidinyl, morpholinyl, tetrahydropyrrolyl or other tertiary amine structures. The weakly basic derivatives of cabazitaxel of the present invention can be prepared into liposome preparations. The liposome preparation has the characteristics of high drug loading, high encapsulation efficiency, good stability and the like. After injection, it can greatly increase the circulation time of the drug in the body, increase the accumulation of the drug in the tumor site, and improve the anti-tumor effect and tolerance dose. .

The invention discloses a dual-ring hairpin probe mediate label-free strand displacement amplification method for detecting bleomycin. The method includes the steps that when bleomycin exists, a dual-ring hairpin probe fractures at a recognition site, and a triggering sequence is released; the triggering sequence and a ring part of a signal probe are combined and subjected to a strand displacement amplification reaction under the effect of a polymerase and a nicking enzyme, and finally a great number of G-four-chain sequences are generated. At the same time, a primer chain extends to open the signal probe, and therefore the G-four-chain sequences packaged in the neck of the signal probe can be exposed. Finally, NMM molecules are bound to the G-four-chain sequences to generate fluorescence signals, and bleomycin is quantified through the detected fluorescence signals. As the triggering sequence is designed at the neck of the dual-ring hairpin probe, background signals of a detection system are reduced; by combining bleomycin cutting and the strand displacement amplification reaction, bleomycin can be detected sensitively, and the detection limit is 0.34 nm. The method has the advantages of being easy to operate, free of labeling and good in specificity.

The invention belongs to the field of pharmaceutical preparations, and discloses a chitosan lipoprotein intranasal administration nanocomposite, comprising lipoprotein nanoparticles, chitosan or / and chitosan derivatives having the effect of promoting absorption of nasal mucosa. The invention also discloses a preparation method of chitosan lipoprotein intranasal administration nanocomposite, comprising: self-assembling chitosan or / and chitosan derivatives and lipoprotein nanoparticles through dynamic force to form a good membrane permeability nasal preparations. The preparation condition of the chitosan lipoprotein intranasal administration nanocomposite is mild, the process is simple, and the industrialized production is easy. The system is safe, non-toxic, and has good biodegradability, which can improve the absorption of lipoprotein drugs in the nasal mucosa and ensure high-efficiency brain targeting. The dosage form is administered by nasal drops, sprays, etc., and is easy to operate, convenient for patients who take the medicine for a long time, and has a good clinical application prospect in the treatment of central nervous system diseases.

The application provides a capsule endoscope system, including: a capsule endoscope and an external locator; the capsule endoscope includes a capsule endoscope body, the capsule endoscope body includes a capsule endoscope shell and a capsule endoscope magnet, and the capsule endoscope magnet is set Inside the shell of the capsule endoscope; the external locator includes an external magnet; the capsule endoscope magnet and the external magnet are respectively arranged on the inside and outside of the site to be tested, and are fixed on the site to be tested by mutual attraction, or the external magnet moves to make the inside of the capsule endoscopic. The mirror magnet moves at the site to be tested; the capsule endoscope also includes a connecting tube, one end of the connecting tube is connected to the outer surface of the capsule endoscope shell, and the other end of the connecting tube is suspended in the air; the connecting tube is used for washing the capsule endoscope and for recovering the capsule Endoscopy. By adopting the capsule endoscope system provided by the present invention, doctors can detect blunt abdominal injuries in real time, quickly, directly and accurately, with simple structure, easy operation and great clinical application potential.

The invention relates to a protein-type nanoparticle for multispecific antibody delivery and its application and preparation method. The protein-type nanoparticles include polyester and a protein having a hydrophobic domain, and the hydrophobic domain of the protein is combined with the polyester through hydrophobic interactions; the protein is at least one of albumin, globulin and cell wall protein A sort of. The protein-type nanoparticles of the present invention have excellent stability and biocompatibility, and can prepare a multispecific antibody delivery platform (αFc-NP) by bonding anti-IgG-Fc antibodies or anti-IgG-Fc antibody fragments, αFc-NP It can stably, quickly and easily bind to multiple specific antibodies through antigen-antibody interaction and enhance the therapeutic effect of specific antibodies.