Method for detecting DNA mutation by nanometer gold

Abstract

The invention discloses a DNA mutation test method in use of nano-Au, relates to a DNA mutation test method, and provides a DNA mutation test method that uses a nano-Au probe. The DNA mutation test method leads wild single-stranded DNA, a probe that is completely complementary with a wild single-stranded DNA sequence and the nano-Au to be mixed, statically placed, added with a sodium chloride solution, and then statically placed, thus obtaining an architecture 1; the DNA mutation test method further leads mutant single-stranded DNA, the probe that is completely complementary with the wild single-stranded DNA sequence and the nano-Au to be mixed, statically placed, added with the sodium chloride solution, and then statically placed, thus obtaining an architecture 2; the sodium chloride solution is added into and evenly mixed with the architecture 1, the ultraviolet-visible absorption spectrum detection is implemented, and an absorbance value that is obtained at a 520nm position is taken as an absorbance value 1; the sodium chloride solution is added into and evenly mixed with the architecture 2, the ultraviolet-visible absorption spectrum detection is implemented, and an absorbance value that is obtained at a 520nm position is taken as an absorbance value 2; when the absorbance values 2 and 1 have marked difference, mutation is determined to exist. The DNA mutation test method does not need any modification of the DNA and the nano-Au, thus simplifying sample processing.

Description

technical field

[0001] The invention relates to a method for detecting DNA mutations, in particular to a method for detecting DNA mutations with nanometer gold probes. Background technique

[0002] Gene mutation refers to the change of base pair composition or sequence in the structure and function of genomic DNA molecules, mainly including base substitution, deletion or insertion. Since gene mutations are closely related to the occurrence of various genetic diseases and tumors, the detection of gene mutations is of great significance for the early detection and diagnosis of genetic diseases or tumors.

[0003] The methods for detecting gene mutation mainly include gel electrophoresis, DNA sequencing and DNA chip technology. The basic principle of gel electrophoresis is: DNA with different conformations has different mobility in the gel, and whether there is a gene mutation can be judged according to the change of the mobility. Although this method is simple, its sensitivi...

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