Multi-crosslinked high-strength enzyme-induced mineralized hydrogel and preparation method and application thereof

A multi-crosslinking, high-strength technology, applied in the field of medical materials, can solve the problems of low swelling stability, high brittleness of mineralized hydrogel, lack of osteogenic bioactivity, etc., to achieve improved mechanical properties, excellent comprehensive mechanical properties, The effect of great research value and clinical application potential

Active Publication Date: 2021-12-24
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a multi-crosslinked high-strength enzyme-induced mineralization hydrogel and its preparation method and application, which can greatly improve the low compressive strength of the original hydrogel At the same time as low swelling stability, it solves the problems of high brittleness and lack of osteogenic bioactivity in existing mineralized hydrogels

Method used

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  • Multi-crosslinked high-strength enzyme-induced mineralized hydrogel and preparation method and application thereof
  • Multi-crosslinked high-strength enzyme-induced mineralized hydrogel and preparation method and application thereof
  • Multi-crosslinked high-strength enzyme-induced mineralized hydrogel and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] 1. Use Ca 2+ , Zn 2+ 、Sr 2+ Preparation of crosslinked unmineralized pristine SA-PAM hydrogels

[0044] (1) Accurately weigh 2g sodium alginate (SA, purchased from Cologne) and 12g acrylamide (AM, purchased from Sigma), measure 80mL of deionized water in a beaker, dissolve AM in deionized water, add SA, place the beaker on a constant temperature water bath stirrer, heat at 45°C overnight until SA is completely dissolved, and record it as solution A.

[0045] (2) Prepare MBA and APS mother liquor: accurately weigh 0.1 g of MBA (methylenebisacrylamide, purchased from Sigma) powder and dissolve in 5 mL of deionized water; weigh 0.1 g of APS (ammonium persulfate, purchased from Sigma) The powder was dissolved in 1 mL of deionized water. The prepared two mother solutions were stored in the dark at room temperature, and were respectively recorded as solution B (MBA) and solution C (APS).

[0046] (3) Under the condition of stirring in a constant temperature water bath at...

Embodiment 2

[0058] 1. Use Ca 2+ 、Sr 2+ Preparation of cross-linked unmineralized SA-PAM hydrogels

[0059] (1) Accurately weigh 1.5g of sodium alginate (SA, purchased from Cologne) and 13.5g of acrylamide (AM, purchased from Sigma), measure 70mL of deionized water in a beaker, and dissolve AM in deionized water , add SA, place the beaker on a constant temperature water bath stirrer, heat at 37°C overnight until SA is completely dissolved, and record it as solution A.

[0060] (2) Prepare MBA and APS mother liquors: accurately weigh 0.15 g of MBA (methylene bisacrylamide, purchased from Sigma) powder and dissolve in 5 mL of deionized water; weigh 0.15 g of APS (ammonium persulfate, purchased from Sigma) The powder was dissolved in 1 mL of deionized water. The prepared two mother solutions were stored in the dark at room temperature, and were respectively recorded as solution B (MBA) and solution C (APS).

[0061] (3) Under the condition of stirring in a constant temperature water bath ...

Embodiment 3

[0073] 1. Use Ca 2+ , Zn 2+ 、Sr 2+ Preparation of cross-linked unmineralized SA-PAM hydrogels

[0074] (1) Accurately weigh 3g sodium alginate (SA, purchased from Cologne) and 12g acrylamide (AM, purchased from Sigma), measure 80mL of deionized water in a beaker, dissolve AM in deionized water, add SA, place the beaker on a constant temperature water bath stirrer, heat at 37°C overnight until SA is completely dissolved, and record it as solution A.

[0075] (2) Prepare MBA and APS mother liquor: accurately weigh 0.1 g of MBA (methylenebisacrylamide, purchased from Sigma) powder and dissolve in 5 mL of deionized water; weigh 0.1 g of APS (ammonium persulfate, purchased from Sigma) The powder was dissolved in 1 mL of deionized water. The prepared two mother solutions were stored in the dark at room temperature, and were respectively recorded as solution B (MBA) and solution C (APS).

[0076] (3) Under the condition of stirring in a constant temperature water bath at 37°C, a...

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Abstract

The invention discloses a multi-crosslinked high-strength enzyme-induced mineralized hydrogel and a preparation method and application thereof. The preparation method comprises the following steps of (1) adding sodium alginate and acrylamide into deionized water, and heating to completely dissolve the sodium alginate to obtain a solution A, (2) mixing an alkaline phosphatase aqueous solution and the solution A, stirring, adding a methylene bisacrylamide aqueous solution and an accelerator, continuously stirring, adding an initiator, continuously stirring, finally degassing, transferring into a mold, and drying under a closed condition to form solid hydrogel, and (3) soaking the solid hydrogel in an ionic solution overnight, then taking out the solid hydrogel, soaking the solid hydrogel in a calcium glycerophosphate mineralizing solution, and mineralizing the solid hydrogel in a dark condition to prepare the multi-crosslinked high-strength enzyme-induced mineralized hydrogel. The hydrogel material prepared by the invention is good in toughness and excellent in mechanical strength, and has relatively good osteogenesis biological activity.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a multi-crosslinked high-strength enzyme-induced mineralization hydrogel and its preparation method and application. Background technique [0002] In the field of biomedical materials-bone tissue engineering, hydrogels have been widely studied due to their good biocompatibility, biodegradability, tunable physicochemical properties, and porous structure similar to extracellular matrix. However, the strength of traditional polysaccharide hydrogels and synthetic hydrogels cannot play the role of buffering stress in daily life before new bone regeneration. Hydrogels used for bone tissue engineering should have high elastic modulus and sufficient toughness. Under physiological conditions, it has excellent swelling stability to support the load on the bone, and has certain biological activity to promote the growth of new bone. [0003] Researchers have developed different me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/00C08J3/24C08J3/075C08L33/26C08L5/04C08L89/00A61L27/16A61L27/20A61L27/22A61L27/50A61L27/52A61L27/58
CPCC08J7/00C08J3/246C08J3/243C08J3/075A61L27/20A61L27/227A61L27/16A61L27/52A61L27/58A61L27/50A61L2430/02C08J2333/26C08J2405/04C08J2489/00C08L5/04C08L89/00C08L33/26Y02P20/54
Inventor 何静郭鲸吴方舒雪东邓欢张钧伟王瑶
Owner SICHUAN UNIV
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