The invention discloses a multi-crosslinked high-strength
enzyme-induced mineralized hydrogel and a preparation method and application thereof. The preparation method comprises the following steps of (1) adding
sodium alginate and
acrylamide into deionized water, and heating to completely dissolve the
sodium alginate to obtain a solution A, (2) mixing an
alkaline phosphatase aqueous solution and the solution A, stirring, adding a
methylene bisacrylamide
aqueous solution and an accelerator, continuously stirring, adding an initiator, continuously stirring, finally degassing, transferring into a mold, and
drying under a closed condition to form
solid hydrogel, and (3) soaking the
solid hydrogel in an
ionic solution overnight, then taking out the
solid hydrogel, soaking the solid hydrogel in a
calcium glycerophosphate mineralizing solution, and mineralizing the solid hydrogel in a dark condition to prepare the multi-crosslinked high-strength
enzyme-induced mineralized hydrogel. The hydrogel material prepared by the invention is good in
toughness and excellent in
mechanical strength, and has relatively good osteogenesis
biological activity.