83 results about "Glycerophosphoric Acid" patented technology

Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1 / RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2 / HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.

The present invention relates, e.g., to a minimal set of protein-coding genes which provides the information required for replication of a free-living organism in a rich bacterial culture medium, wherein (1) the gene set does not comprise the 101 genes listed in Table 2; and / or wherein (2) the gene set comprises the 381 protein-coding genes listed in Table 3 and, optionally, one of more of: a set of three genes encoding ABC transporters for phosphate import (genes MG410, MG411 and MG412; or genes MG289, MG290 and MG291); the lipoprotein-encoding gene MG185 or MG260; and / or the glycerophosphoryl diester phosphodiesterase gene MG293 or MG385.

The invention discloses a method for preparing rhamnolipid by using a semi-solid state fermentation method and application of the rhamnolipid and belongs to the technical field of bioengineering. The method disclosed by the invention comprises the following steps: by taking agricultural wastes such as rapeseed dregs and bran as raw materials, adding nutrient elements such as glycerin and phosphate, performing semi-solid state fermentation by utilizing pseudomonas aeruginosa AB93066 (purchased from CCTCC), thereby obtaining the rhamnolipid. The defoaming operation can be saved, and the obtained fermentation product containing the rhamnolipid can be directly applied to bioremediation of heavy metal contaminated soil after being sterilized. Meanwhile, the rhamnolipid has the effect of inhibiting pathogenic bacteria, and the soil organic matters are increased in the rapeseed dreg fertilized field.

A sodium glycerylphosphate injection is prepared from anhydrous sodium glycerylphosphate, hydrochloric acid and the water for injection through adding concentrated hydrochloric acid to the water for injection at 20-30 deg.C, while stirring, dissolving anhydrous sodium glycerylphosphate in it while stirring, adding the water for injection while stirring, adding hydrochloric acid to regulate pH=7.2-7.6, and adding the water for injection at 35-40 deg.C. Before it is applied, it must be mixed with compound amino acid or glucose injection. The finished injection must be applied within 24 hr.

The invention relates to the technical scheme of organisms, in particular to gene modification application and a bacterial strain obtained through gene modification. MK3-MEP123, sourced from B.subtilis168 and established in a laboratory, is used as an original strain, at first, glycerinum-3-phosphate dehydrogenase (GlpD) is subjected to overexpression, the transformation from glycerinum-3-phosphate to dihydroxyacetone phosphate is improved, and the influence on MK-7 synthesis is inspected; then, dhbB genes are knocked out, consumption of isochorismate is reduced, and the influence on MK-7 synthesis is inspected; and finally the influence of the supply of an oxygen and carbon and nitrogen source on MK-7 synthesis is inspected through 2L shake flask fermentation. A fundamental research and theoretical basis is provided for establishing a high-yield MK-7 bacterial strain of bacillus natto by a metabolic engineering method industrially.

The invention relates to building and application of engineered strains capable of producing xylitol and ethanol at a high temperature simultaneously with a high yield. Concretely, N376F mutant genes of saccharomyces cerevisiae galactose permease (hexose transporter protein of hexose for transporting galactose and glucose) (ScGAL2) are effectively expressed in heat-resistant yeast strains YZJ015 by a gene engineering transformation method; meanwhile, K.marxianus glycerol-3-phosphate dehydrogenase (KmGPD1) genes are removed to cut off a glycerol production branch, so that the obtained strains YZJ119 obtain good capability of efficiently co-utilizing and fermenting glucose and xylose to produce the ethanol and the xylitol at a high temperature (higher than 42 DEG C). Under the condition of 42 DEG C, the YZJ119 can simultaneously use 43.95g / l of xylose and 83.11g / l of glucose to produce 40.43g / l of xylitol and 34.66g / l of ethanol in 28 hours; the production speed of the xylitol is 1.44g / l / h; the production speed of the ethanol is 1.24g / l / h; the yield of the xylitol is 0.92g / g; and the yield of the ethanol is 0.42g / g.

The invention provides methods and compositions for differentiating stromal cells from adipose tissue into cells having osteoblastic properties, and methods for improving a subject's bone structure. The methods comprise culturing stromal cells from adipose tissue in β-glycerophosphate and ascorbic acid and / or ascorbate-2-phosphate for a time sufficient to allow differentiation of said cells into osteoblasts. Such methods and compositions are useful in the production of osteoblasts for autologous transplantation into bone at a surgical site or injury. The compositions comprise adipose stromal cells, a medium capable of supporting the growth of fibroblasts and amounts of β-glycerophosphate and ascorbic acid and / or ascorbic-2 phosphate sufficient to induce the differentiation of said stromal cells into osteoblasts. The invention further provides methods of identifying compounds that affect osteoblast differentiation. Such compounds are useful in the study of bone development and in the treatment of bone disorders, including bone fractures and osteoporosis.

The invention discloses a cosmetic composition with the long-lasting soothing and repairing effect and application thereof. The cosmetic composition comprises 0.5-5% of panthenol, 0.25-2.5% of myrothamnus flabellifolia leaf / stem extract, 0.01-0.1% of glycerophosphoinositide choline salt and 0.01-0.1% of dunaliella salina extract. Glyceryl glucoside is an active ingredient of the myrothamnus flabellifolia leaf / stem extract, and the myrothamnus flabellifolia leaf / stem extract is an enantiomer of the glyceryl glucoside. The cosmetic composition containing the panthenol, the myrothamnus flabellifolia leaf / stem extract, the glycerophosphoinositide choline salt and the dunaliella salina extract can immediately soothe skin allergic reactions, improve skin tolerance and reduce skin redness causedby irritation, and is mild and safe. The composition with the soothing and repairing effect can relieve skin's lipid metabolism imbalance caused by irritation when being added into cosmetics, therebybeing beneficial to recovery of essential functions. The skin's barrier function can be recovered, the entry of irritants into deep layers of the skin is reduced, moisture volatilization is decreased,and the skin is restored to a healthy state.

The invention provides a CectGPDH2 gene and an application thereof in increase of fatty acid content. The nucleotide sequence of the CectGPDH2 gene is shown as SEQ NO.1. The gene comes from Chlorella ellipsoidea and encodes GPDH, wherein the GPDH participates in shuttle of the mitochondrion G3P (glycerol-3-phosphate), provides electrons for respiratory chains, is a key enzyme for G3P synthesis, is one of the key enzymes for connecting glucose metabolism with lipid metabolism and plays an important role in lipid synthesis and energy metabolism in plants. The total fatty acid content of seeds of Arabidopsis thaliana and rape genetically modified with the gene can be increased obviously, and the gene can be used for increasing the oil content of plant cells and the content of linoleic acid (C18:2) and eicosenoic acid (C20:1) in the plants and has good application prospect.

The invention relates to a cartilage repair temperature-sensitive gel for injection. The gel comprises the following components: chitosan, collagen, beta-sodium glycerophosphate and osteoblast factors. The temperature-sensitive gel provided by the invention is based on blending of chitosan and beta-glyceryl phosphate, and simultaneously collagen and cell growth factors are added, so that the temperature-sensitive gel not only has functions of injectability, biocompatibility, biodegradability and the like in cartilage defect repair, but also provides a powerful growth space and an induced directional differentiation function for osteoblasts. The temperature sensitive gel provided by the invention is simple to use and operate, has a good cartilage repair effect, can shorten rehabilitation time and greatly relieving pain of patients.

The invention belongs to the technical field of antibiosis, particularly relates to an antibacterial silver ion compound, which further relates to a non-irritant silver ion antibacterial agent as wellas a preparation method and an application thereof. The cationic ions in the antibacterial silver ion compound are coordination cations formed by silver ions and amino acid ligands. The amino acid ligand is selected from amino acid and / or polypeptide, and the anion is selected from one or more of acetate radical, hydrogen phosphate radical, dihydrogen phosphate radical, glycerol hydrogen phosphate radical, glycerol phosphate radical, lactate radical, glycolate radical, tartrate radical, citrate radical and malate radical. The antibacterial silver ion compound disclosed by the invention has good stability and compatibility, does not generate decomposition discoloration after being placed for a long time, does not have unpleasant odor, has good salt tolerance and is non-irritant. The antibacterial agents such as antibacterial liquid, antibacterial gel and the like prepared by taking the antibacterial silver ion compound as an active ingredient have better antibacterial property, are non-irritant and can be in direct contact with a human body.

The invention belongs to the technical field of biological fermentation, and discloses a method for producing vitamin B12 by using Pseudomonas aeruginosa based on pH value control. The method for producing the vitamin B12 by using the Pseudomonas aeruginosa based on the pH value control comprises the steps that a production strain of vitamin B12, namely the Pseudomonas aeruginosa is inoculated into a fermentation medium, and fermentation is carried out at a temperature of 25-30 DEG C; the composition of the fermentation medium comprises 70-85g / L of cane molasses, 90-110g / L of yeast extract, 0.05-0.2 g / L of 5,6-dimethylbenzimidazole, 0.1-1g / L of glycerophosphoric acid, 0.05-2g / L of cobalt chloride, 0.05-0.2g / L of urea, 10-30g / L of betaine, 0.05-0.2 g / L of arginine, 0.01-0.5g / L of thiamine and the like; the pH value of fermentation broth is controlled at 7 by the cane molasses and the urea; and the purified vitamin B12 is separated from the fermentation broth. The yield of the vitamin B12 is improved by controlling the pH in the fermentation process.

The invention belongs to the technical field of gene engineering of enzymes, and discloses a preparation method of high-purity glycerophosphodiesters, which comprises the following steps: hydrolyzing lysophosphatide in a water phase system by taking lysophosphatide as a raw material and pfGDPD treated by a metal ion chelating agent as a catalyst to obtain a glycerophosphodiester product; the amino acid sequence of the pfGDPD is shown as SEQ ID No: 1. Starting from lysophospholipid, high-purity glycerophosphodiesters (such as GPC, GPE, GPI and the like) can be prepared through one step of hydrolysis by a single enzyme method, and the conversion rate is close to 100%. The invention provides a new thought and method for the production of glycerophosphate diester.

The invention provides a Cem GPDH (Glycerol-3-phosphate dehydrogenase) gene and an application of the Cem GPDH gene in the aspect of increase of cellular fatty acid content. A nucleotide sequence of the Cem GPDH gene is represented as SEQ ID NO: 1. The gene is derived from Chlorella ellipsoidea, and GPDH is coded. G3P (Glycerol-3-phosphate) synthesized through catalysis by GPDH is an important raw material for synthesis of TAG (triacylglycerol), the enzyme participates in mitochondria G3P shuttle, provides electrons for a respiratory chain, is a key enzyme for G3P synthesis, is one of key enzymes for connecting glycometabolism and lipid metabolism and has an important effect on lipid synthesis and energy metabolism in plants; and besides, the gene can remarkably increase the total fatty acid content of cells when utilized to convert yeast cells, plant cells and microalgae cells.

The invention relates to a hydrogel for repairing peripheral nerve injury (PNI). The hydrogel contains the following components in percentages by mass: 0.2%-0.6% of hyaluronic acid, 0.18%-0.22% of hydroxyethyl cellulose sodium, 1.5%-2.5% of chitosan, 2.5%-3.5% of beta-glycerophosphoric acid disodium salt, 0.8%-1.2% of PLGA drug-carrying microspheres, and the balance of water. The hydrogel providedby the invention uses CS-HEC-HA / GP as a substrate, overcomes the shortcomings of a single component HA scaffold, improves the mechanical properties of the substrate, controls the gelation time and degradation, belongs to temperature-sensitive hydrogels, and can be used for injection administration; and in addition, a nerve growth factor (NGF) is loaded into a biological material, so that targetedand sustained molecular release can be realized directly in tissue repair, and the NGF is loaded into the PLGA microspheres to obtain PLGA (NGF) with a uniform particle size to achieve the effect ofdrug sustained release and prolong the treatment cycle by the hydrogel, so that side effects caused by drug burst release can be avoided.

The invention relates to recombinant escherichia coli capable of producing O-acetyl-L-homoserine at high yield and application of the recombinant escherichia coli in preparation of O-acetyl-L-homoserine by microbial fermentation. The method comprises the following steps that knocking out of a gene glpR responsible for encoding a repression protein in a glycerol metabolic pathway is carried out, sothat constitutive expression of a glycerol kinase gene glpK and a glycerol 3-phosphate dehydrogenase gene is realized, and the flux of the glycerol metabolic pathway of a strain is enhanced; secondly, the gene is found to be a key gene in a glycerol metabolic pathway of the strain by overexpression of glpD gene; and finally, an in-situ promoter of the glpD gene is replaced with a trc promoter toindicate that the level of O-acetyl-L homoserine produced by strain fermentation is closely related to the expression level of the glpD gene. Through the combination of the modification strategies, the escherichia coli strain capable of producing the O-acetyl-L-homoserine at high yield is obtained.

The invention relates to the field of cosmetics, and particularly discloses a skin care composition. The skin care composition comprises the following active ingredients: [beta]-glucan, glycerophosphoric acid inositol choline salts, radix ophiopogonis extracts, opuntia streptacantha stem extracts and radix sophorae flavescentis extracts. Compared with the prior art, through a mutual function, i.e., a synergistic interaction function, of all ingredients, on one hand, the composition provided by the invention can improve the moisture content of the skin and improve skin immunity, on the other hand, the composition provided by the invention alleviates skin discomfortableness caused by acute inflammations, reduces skin aging acceleration caused by chronic inflammations, causes the skin to be soft, moist and comfortable, and has a good and long-lasting effect of moisturizing and alleviating the sensitive skin.

The invention provides a culture medium for inducing differentiation of dental pulp stem cells into osteoblasts. The culture medium comprises 4-5.5g / L of bovine serum albumin (BSA), 1.5mg / L of reducedglutathione, 0.08-0.12mmol / L of beta-mercaptoethanol, 0.08%-1.5%(V / V) of SITE, 0.8-1.2M / L of beta-glycerophosphoric acid, 15-25[mu]M / L of L-ascorbic acid, 0.5-1.2mM / L of dexamethasone and the balanceof low-sugar dulbecco's modified eagle medium (DMEM) culture medium added with an amino acid concentrated solution. The culture medium for inducing differentiation of dental pulp stem cells to osteoblasts is a serum-free culture medium, and the culture medium can be used to optimize a method for inducing differentiation of dental pulp stem cells to osteoblasts. The induction time is short and cost is low, and the invention provides a new selection for culture of seed cells in bone tissue engineering.

The invention discloses a synthesis method of mitochondria-targeting nano triphenylphosphine MoS2 quantum dots. The preparation method comprises the following steps: firstly, combining amine-modified1 2-distearoyl-sn-glycerin-3phosphoric acid ethanolamine-n-[amino-(polyethylene glycol)-2000] and (3-carboxylpropyl)-triphenylphosphine bromide together through an amino coupling reaction by using a cross-linking agent N-(3-dimethylaminopropyl)-N-ethyl diimine hydrochloride with the length of zero to form DSPE-PEG-GTPP; then, mixing the DSPE-PEG-GTPP and the molybdenum disulfide quantum dot in trichloromethane, wherein the hydrophobic end distearoyl phosphatidyl ethanolamine of the DSPE-PEG-GTPP is connected to the surface of the molybdenum disulfide quantum dot, the (3-carboxylpropyl)-triphenylphosphine bromide end extends outwards, and thus the triphenylphosphine molybdenum disulfide is obtained. The synthesized triphenylphosphine molybdenum disulfide quantum dot can target mitochondria,and a new way is provided for research of mitochondria-related nervous system diseases and mechanisms.

The invention discloses a nano drug delivery system of a grassland spodoptera litura sex attractant and application of the nano drug delivery system. The amphiphilic lipid molecule loaded sex pheromone and the synergist linalool are formed by self-assembly of amphiphilic lipid molecules DSPE-mPEG5000 (1, 2-distearoyl-sn-glycerin-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000]), sex pheromone and linalool through a cosolvent method, and then the amphiphilic lipid molecule loaded sex pheromone and the synergist linalool are adsorbed onto a sustained-release carrier 0.22 [mu]m polyether sulfone membrane. The sex pheromone is composed of (Z)-9-tetradecene-1-alcohol acetate, (Z)-7-dodecene-1-alcohol acetate and (Z)-11-hexadecene-1-alcohol acetate in a mass ratio of 90: 0.5: 9.5. Theinvention further discloses a moth luring effect of the moth luring agent as a grassland spodoptera litura sex luring agent in the field.

One aspect of the present invention relates to stabilized irrigating compositions comprising sodium glycerophosphate and / or calcium glycerophosphate, and a bicarbonate salt. Another aspect of the present invention relates to methods of irrigating ocular tissues during a surgical procedure comprising bathing the intraocular tissues with an irrigating composition comprising sodium glycerophosphate and / or calcium glycerophosphate, and a bicarbonate salt.

The invention discloses a sustained-release microneedle patch and a preparation method thereof, and relates to the technical field of medicines. The microneedle patch comprises a needle body and a needle tip on the needle body, and at least the needle tip part comprises a part which is prepared from needle tip preparation liquid and can realize gelation in the drying process of the microneedle patch; and solutes of the needle tip preparation liquid comprise glycerophosphate compounds and nonionic cellulose ether compounds. The glycerophosphate and the cellulose ether compound can compete for water molecules, so that the cloud point of the cellulose ether compound is changed, the microneedle is gelatinized in the drying process, the gel microneedle with a good skin puncture effect is prepared, and in-vivo slow release of entrapped drugs is realized.

The invention discloses natural polysaccharide-based injectable in-situ formed hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: 1) dissolving a natural polysaccharide material in a neutral or acidic solution to prepare a natural polysaccharide material solution; 2) dissolving glycerophosphate in water or dilute acid to preparea glycerophosphate solution; (3) dissolving four-arm polyethylene glycol succinimide carbonate in a PBS to prepare a four-arm polyethylene glycol succinimide carbonate solution; and 4) uniformly mixing the solutions prepared in the step 1), the step 2) and the step 3), and performing incubating to obtain the natural polysaccharide-based injectable in-situ formed hydrogel. According to the preparation method, rapid forming is achieved through a click chemical reaction, gelation can be achieved through operation under physiological conditions, the gelation time is short, the reaction conditionsare mild, and the gelation performance is controllable. The hydrogel prepared by the method can be applied to tissue engineering materials, and is particularly suitable for local cavity filling materials for bone defects.

Glycerophosphate salts have been found to hasten the healing and rapid scaling of lesions of the mouth. Methods are provided for improving healing of an oral lesion or preventing diseases or conditions related to an oral lesion using a glycerophosphate salt. In particular, methods are provided for preventing diseases or conditions related to an oral lesion using calcium glycerophosphate (CGP).

The invention discloses a bonding agent for decayed tooth filling treatment and a preparing method thereof. The bonding agent comprises, by weight, 34-52 parts of epoxy resin, 5-13 parts of sodium hexafluorisilicate, 6-19 parts of glycerophosphoric acid methacrylate, 12-17 parts of hydroxyethyl cellulose, 15-22 parts of talcum powder, 32-43 parts of glycerinum, 3-8 parts of methacrylic acid, 12-25 parts of dibutene and 6-17 parts of triethanolamine. The prepared bonding agent is high in bonding strength, good in compressive property and not prone to being dissolved in saliva.

The invention relates to the technical field of materials, and in particular relates to a temperature-sensitive hydrogel with visual diagnosis and treatment of an infected wound and a preparation method of the hydrogel. The preparation method comprises the following steps: firstly mixing chitosan and an acid containing an indicator to obtain a mixed solution A, secondly mixing beta-glycerophosphoric acid disodium salt and a solution containing a photothermal material to obtain a mixed solution B, and finally mixing the mixed solution A and the mixed solution B to obtain the indicator and photothermal material doped temperature-sensitive hydrogel. The temperature-sensitive hydrogel obtained by the method can detect whether the wound is infected in situ through naked-eye visible color change, the color of the hydrogel at the wound infection site changes from green to yellow, and after the wound is confirmed to be infected, heat generated by illumination is used to treat the infected site; the adopted preparation method is simple to operate, the hydrogel can be used for diagnosis without the aid of instrument; and the material has higher application value in antibacterial diagnosis and treatment.