79 results about "Glycerophosphoric Acid" patented technology

Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.

A sodium glycerylphosphate injection is prepared from anhydrous sodium glycerylphosphate, hydrochloric acid and the water for injection through adding concentrated hydrochloric acid to the water for injection at 20-30 deg.C, while stirring, dissolving anhydrous sodium glycerylphosphate in it while stirring, adding the water for injection while stirring, adding hydrochloric acid to regulate pH=7.2-7.6, and adding the water for injection at 35-40 deg.C. Before it is applied, it must be mixed with compound amino acid or glucose injection. The finished injection must be applied within 24 hr.

The invention provides a CectGPDH2 gene and an application thereof in increase of fatty acid content. The nucleotide sequence of the CectGPDH2 gene is shown as SEQ NO.1. The gene comes from Chlorella ellipsoidea and encodes GPDH, wherein the GPDH participates in shuttle of the mitochondrion G3P (glycerol-3-phosphate), provides electrons for respiratory chains, is a key enzyme for G3P synthesis, is one of the key enzymes for connecting glucose metabolism with lipid metabolism and plays an important role in lipid synthesis and energy metabolism in plants. The total fatty acid content of seeds of Arabidopsis thaliana and rape genetically modified with the gene can be increased obviously, and the gene can be used for increasing the oil content of plant cells and the content of linoleic acid (C18:2) and eicosenoic acid (C20:1) in the plants and has good application prospect.

The invention relates to a cartilage repair temperature-sensitive gel for injection. The gel comprises the following components: chitosan, collagen, beta-sodium glycerophosphate and osteoblast factors. The temperature-sensitive gel provided by the invention is based on blending of chitosan and beta-glyceryl phosphate, and simultaneously collagen and cell growth factors are added, so that the temperature-sensitive gel not only has functions of injectability, biocompatibility, biodegradability and the like in cartilage defect repair, but also provides a powerful growth space and an induced directional differentiation function for osteoblasts. The temperature sensitive gel provided by the invention is simple to use and operate, has a good cartilage repair effect, can shorten rehabilitation time and greatly relieving pain of patients.

The invention belongs to the technical field of antibiosis, particularly relates to an antibacterial silver ion compound, which further relates to a non-irritant silver ion antibacterial agent as wellas a preparation method and an application thereof. The cationic ions in the antibacterial silver ion compound are coordination cations formed by silver ions and amino acid ligands. The amino acid ligand is selected from amino acid and/or polypeptide, and the anion is selected from one or more of acetate radical, hydrogen phosphate radical, dihydrogen phosphate radical, glycerol hydrogen phosphate radical, glycerol phosphate radical, lactate radical, glycolate radical, tartrate radical, citrate radical and malate radical. The antibacterial silver ion compound disclosed by the invention has good stability and compatibility, does not generate decomposition discoloration after being placed for a long time, does not have unpleasant odor, has good salt tolerance and is non-irritant. The antibacterial agents such as antibacterial liquid, antibacterial gel and the like prepared by taking the antibacterial silver ion compound as an active ingredient have better antibacterial property, are non-irritant and can be in direct contact with a human body.

The invention belongs to the technical field of biological fermentation, and discloses a method for producing vitamin B12 by using Pseudomonas aeruginosa based on pH value control. The method for producing the vitamin B12 by using the Pseudomonas aeruginosa based on the pH value control comprises the steps that a production strain of vitamin B12, namely the Pseudomonas aeruginosa is inoculated into a fermentation medium, and fermentation is carried out at a temperature of 25-30 DEG C; the composition of the fermentation medium comprises 70-85g/L of cane molasses, 90-110g/L of yeast extract, 0.05-0.2 g/L of 5,6-dimethylbenzimidazole, 0.1-1g/L of glycerophosphoric acid, 0.05-2g/L of cobalt chloride, 0.05-0.2g/L of urea, 10-30g/L of betaine, 0.05-0.2 g/L of arginine, 0.01-0.5g/L of thiamine and the like; the pH value of fermentation broth is controlled at 7 by the cane molasses and the urea; and the purified vitamin B12 is separated from the fermentation broth. The yield of the vitamin B12 is improved by controlling the pH in the fermentation process.

The invention belongs to the technical field of gene engineering of enzymes, and discloses a preparation method of high-purity glycerophosphodiesters, which comprises the following steps: hydrolyzing lysophosphatide in a water phase system by taking lysophosphatide as a raw material and pfGDPD treated by a metal ion chelating agent as a catalyst to obtain a glycerophosphodiester product; the amino acid sequence of the pfGDPD is shown as SEQ ID No: 1. Starting from lysophospholipid, high-purity glycerophosphodiesters (such as GPC, GPE, GPI and the like) can be prepared through one step of hydrolysis by a single enzyme method, and the conversion rate is close to 100%. The invention provides a new thought and method for the production of glycerophosphate diester.

The invention provides a Cem GPDH (Glycerol-3-phosphate dehydrogenase) gene and an application of the Cem GPDH gene in the aspect of increase of cellular fatty acid content. A nucleotide sequence of the Cem GPDH gene is represented as SEQ ID NO: 1. The gene is derived from Chlorella ellipsoidea, and GPDH is coded. G3P (Glycerol-3-phosphate) synthesized through catalysis by GPDH is an important raw material for synthesis of TAG (triacylglycerol), the enzyme participates in mitochondria G3P shuttle, provides electrons for a respiratory chain, is a key enzyme for G3P synthesis, is one of key enzymes for connecting glycometabolism and lipid metabolism and has an important effect on lipid synthesis and energy metabolism in plants; and besides, the gene can remarkably increase the total fatty acid content of cells when utilized to convert yeast cells, plant cells and microalgae cells.

The invention relates to recombinant escherichia coli capable of producing O-acetyl-L-homoserine at high yield and application of the recombinant escherichia coli in preparation of O-acetyl-L-homoserine by microbial fermentation. The method comprises the following steps that knocking out of a gene glpR responsible for encoding a repression protein in a glycerol metabolic pathway is carried out, sothat constitutive expression of a glycerol kinase gene glpK and a glycerol 3-phosphate dehydrogenase gene is realized, and the flux of the glycerol metabolic pathway of a strain is enhanced; secondly, the gene is found to be a key gene in a glycerol metabolic pathway of the strain by overexpression of glpD gene; and finally, an in-situ promoter of the glpD gene is replaced with a trc promoter toindicate that the level of O-acetyl-L homoserine produced by strain fermentation is closely related to the expression level of the glpD gene. Through the combination of the modification strategies, the escherichia coli strain capable of producing the O-acetyl-L-homoserine at high yield is obtained.

The invention provides a culture medium for inducing differentiation of dental pulp stem cells into osteoblasts. The culture medium comprises 4-5.5g/L of bovine serum albumin (BSA), 1.5mg/L of reducedglutathione, 0.08-0.12mmol/L of beta-mercaptoethanol, 0.08%-1.5%(V/V) of SITE, 0.8-1.2M/L of beta-glycerophosphoric acid, 15-25[mu]M/L of L-ascorbic acid, 0.5-1.2mM/L of dexamethasone and the balanceof low-sugar dulbecco's modified eagle medium (DMEM) culture medium added with an amino acid concentrated solution. The culture medium for inducing differentiation of dental pulp stem cells to osteoblasts is a serum-free culture medium, and the culture medium can be used to optimize a method for inducing differentiation of dental pulp stem cells to osteoblasts. The induction time is short and cost is low, and the invention provides a new selection for culture of seed cells in bone tissue engineering.

The invention discloses a nano drug delivery system of a grassland spodoptera litura sex attractant and application of the nano drug delivery system. The amphiphilic lipid molecule loaded sex pheromone and the synergist linalool are formed by self-assembly of amphiphilic lipid molecules DSPE-mPEG5000 (1, 2-distearoyl-sn-glycerin-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000]), sex pheromone and linalool through a cosolvent method, and then the amphiphilic lipid molecule loaded sex pheromone and the synergist linalool are adsorbed onto a sustained-release carrier 0.22 [mu]m polyether sulfone membrane. The sex pheromone is composed of (Z)-9-tetradecene-1-alcohol acetate, (Z)-7-dodecene-1-alcohol acetate and (Z)-11-hexadecene-1-alcohol acetate in a mass ratio of 90: 0.5: 9.5. Theinvention further discloses a moth luring effect of the moth luring agent as a grassland spodoptera litura sex luring agent in the field.

The invention discloses a sustained-release microneedle patch and a preparation method thereof, and relates to the technical field of medicines. The microneedle patch comprises a needle body and a needle tip on the needle body, and at least the needle tip part comprises a part which is prepared from needle tip preparation liquid and can realize gelation in the drying process of the microneedle patch; and solutes of the needle tip preparation liquid comprise glycerophosphate compounds and nonionic cellulose ether compounds. The glycerophosphate and the cellulose ether compound can compete for water molecules, so that the cloud point of the cellulose ether compound is changed, the microneedle is gelatinized in the drying process, the gel microneedle with a good skin puncture effect is prepared, and in-vivo slow release of entrapped drugs is realized.

The invention discloses natural polysaccharide-based injectable in-situ formed hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: 1) dissolving a natural polysaccharide material in a neutral or acidic solution to prepare a natural polysaccharide material solution; 2) dissolving glycerophosphate in water or dilute acid to preparea glycerophosphate solution; (3) dissolving four-arm polyethylene glycol succinimide carbonate in a PBS to prepare a four-arm polyethylene glycol succinimide carbonate solution; and 4) uniformly mixing the solutions prepared in the step 1), the step 2) and the step 3), and performing incubating to obtain the natural polysaccharide-based injectable in-situ formed hydrogel. According to the preparation method, rapid forming is achieved through a click chemical reaction, gelation can be achieved through operation under physiological conditions, the gelation time is short, the reaction conditionsare mild, and the gelation performance is controllable. The hydrogel prepared by the method can be applied to tissue engineering materials, and is particularly suitable for local cavity filling materials for bone defects.

A compound represented by the following general formula (I), or a salt thereof:

wherein R¹ and R² independently represent a substituted or unsubstituted alkyl or alkenyl group having 8 to 30 carbon atoms; L represents a divalent bridging group (L is constituted by atoms selected from the group consisting of carbon atom, oxygen atom, nitrogen atom and hydrogen atom, wherein the total number of atoms constituting L and selected from the group consisting of carbon atom, oxygen atom and nitrogen atom is 4 to 15); and Ch represents a chelate forming moiety containing 3 or more nitrogen atoms. A compound suitable for a liposome contrast medium for performing lesion-selective imaging is provided.

The invention relates to the technical field of materials, and in particular relates to a temperature-sensitive hydrogel with visual diagnosis and treatment of an infected wound and a preparation method of the hydrogel. The preparation method comprises the following steps: firstly mixing chitosan and an acid containing an indicator to obtain a mixed solution A, secondly mixing beta-glycerophosphoric acid disodium salt and a solution containing a photothermal material to obtain a mixed solution B, and finally mixing the mixed solution A and the mixed solution B to obtain the indicator and photothermal material doped temperature-sensitive hydrogel. The temperature-sensitive hydrogel obtained by the method can detect whether the wound is infected in situ through naked-eye visible color change, the color of the hydrogel at the wound infection site changes from green to yellow, and after the wound is confirmed to be infected, heat generated by illumination is used to treat the infected site; the adopted preparation method is simple to operate, the hydrogel can be used for diagnosis without the aid of instrument; and the material has higher application value in antibacterial diagnosis and treatment.

The invention discloses a preparation method of a temperature-sensitive self-curing bacteriostatic medical ultrasonic coupling agent, including the following steps: Step 1, preparing a chitosan solution; Step 2, preparing beta-glycerophosphate; and Step 3, preparing a finished product. The prepared coupling agent has approximately equalacoustic impedance of an existing coupling agent, completely excludes air, can be kept for a long time without drying, remains sticky for a long time, does not irritate the skin or cause damage to the skin, can inhibit bacterial growth and prevent cross infection, is easy to clean or easy to remove, and can be widely used as a B-ultrasound coupler.