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Cartilage repair temperature-sensitive gel for injection and preparation method thereof

A technology of cartilage repair and temperature-sensitive gel, which is applied in the field of biomedicine, can solve the problems of precipitation temperature-sensitivity deterioration, achieve stable quality, good effect, and relieve pain

Active Publication Date: 2020-04-28
河北柯瑞生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, in the preparation process of temperature-sensitive gel, chitosan is usually first dissolved in an acidic solution, and β-glycerophosphate sodium salt is added under acidic conditions to obtain a temperature-sensitive gel system. However, adding collagen and / or After bone morphogenetic protein (BMP), there will be problems such as precipitation and / or poor temperature sensitivity, and there is no relevant report in the prior art

Method used

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  • Cartilage repair temperature-sensitive gel for injection and preparation method thereof
  • Cartilage repair temperature-sensitive gel for injection and preparation method thereof
  • Cartilage repair temperature-sensitive gel for injection and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Prepare 200ml of 0.1mol / L phosphate buffer solution, slowly add 3g of chitosan (deacetylation degree 95%) under stirring conditions, stir at room temperature 25°C for 24h, then centrifuge the solution at high speed, remove the precipitate and obtain the initial concentration of 0.015g / ml chitosan solution.

[0035] Take 0.1 g of the initial collagen solution with an initial concentration of 0.001 g / ml, add it to the above 200 ml chitosan solution, stir for 24 hours, and let stand at 4° C. for 48 hours to prepare a mixture of chitosan and collagen.

[0036] To prepare 0.60g / ml sodium β-glycerophosphate solution, take 200μL chitosan and collagen mixture into a 1ml centrifuge tube, and add 50μL sodium β-glycerophosphate solution dropwise slowly at 4°C under agitation Add the above mixture solution to make the final concentration of β-sodium glycerophosphate solution 0.12g / ml.

[0037]Then, under the condition of stirring at 0°C, osteoblast factor was slowly added dropwise...

Embodiment 2

[0039] Prepare 200ml of 0.1mol / L phosphate buffer solution, slowly add 4g of chitosan (deacetylation degree 95%) under stirring conditions, stir at room temperature 25°C for 24h, then centrifuge the solution at high speed, remove the precipitate and obtain the initial concentration of 0.02g / ml chitosan solution.

[0040] Take 0.2 g of the initial collagen solution with an initial concentration of 0.001 g / ml, add it to the above-mentioned 200 ml chitosan solution, stir for 24 h, and let stand at 4° C. for 48 h to prepare a mixture of chitosan and collagen.

[0041] To prepare 0.60g / ml sodium β-glycerophosphate solution, take 200μL chitosan and collagen mixture and put it in a 1ml centrifuge tube, and slowly add 100μL sodium β-glycerophosphate solution dropwise at 4°C with stirring Add to the above mixture solution to make the final concentration of β-sodium glycerophosphate solution 0.2g / ml.

[0042] Then, under the condition of stirring at 0°C, osteoblast factor was slowly ad...

Embodiment 3

[0044] Prepare 200ml of 0.1mol / L phosphate buffer solution, slowly add 6g of chitosan (deacetylation degree 95%) under stirring conditions, stir at room temperature 25°C for 24h, then centrifuge the solution at high speed, and remove the precipitate to obtain an initial concentration of 0.03g / ml chitosan solution.

[0045] Take 0.3 g of the initial collagen solution with an initial concentration of 0.001 g / ml, add it to the above-mentioned 200 ml chitosan solution, stir for 24 hours, and let stand at 4° C. for 48 hours to prepare a mixture of chitosan and collagen.

[0046] To prepare 0.60g / ml sodium β-glycerophosphate solution, take 200μL chitosan and collagen mixture and put it in a 1ml centrifuge tube, and slowly add 200μL sodium β-glycerophosphate solution dropwise at 4°C under stirring condition Add the above mixture solution to make the final concentration of β-sodium glycerophosphate solution 0.3g / ml.

[0047] Then, under the condition of stirring at 4°C, osteoblast fa...

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Abstract

The invention relates to a cartilage repair temperature-sensitive gel for injection. The gel comprises the following components: chitosan, collagen, beta-sodium glycerophosphate and osteoblast factors. The temperature-sensitive gel provided by the invention is based on blending of chitosan and beta-glyceryl phosphate, and simultaneously collagen and cell growth factors are added, so that the temperature-sensitive gel not only has functions of injectability, biocompatibility, biodegradability and the like in cartilage defect repair, but also provides a powerful growth space and an induced directional differentiation function for osteoblasts. The temperature sensitive gel provided by the invention is simple to use and operate, has a good cartilage repair effect, can shorten rehabilitation time and greatly relieving pain of patients.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an injectable thermosensitive gel for cartilage repair and a preparation method thereof. Background technique [0002] Cartilage is mainly composed of scattered round or oval chondrocytes and dense extracellular matrix, and is a tissue without blood vessels, nerves, and lymph glands. Its extracellular matrix is ​​mainly composed of type II collagen network structure and feathery glycoproteins, which provide sufficient mechanical strength for cartilage tissue. This structural feature makes the self-repair ability of damaged cartilage tissue very limited. However, damage or loss of function of cartilage tissue is common in surgery. With the aging of the population and the growing obesity problem, the number of people suffering from arthritis is increasing every year, and there are also many people who suffer from joint cartilage defects due to excessive exercise. ...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/20A61L27/22A61L27/50A61L27/52A61L27/58
CPCA61L27/24A61L27/20A61L27/227A61L27/50A61L27/52A61L27/58A61L2400/06A61L2430/06A61L2300/412C08L5/08C08L89/00
Inventor 夏中博左明星李杰孙敬超刘鑫齐磊
Owner 河北柯瑞生物医药有限公司
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