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Nano drug delivery system of grassland spodoptera litura sex attractant and application of nano drug delivery system

A technology of Spodoptera frugiperda and nano-drug loading, which is applied in the application, attracting pesticides, biocides, etc., can solve the problems of different attracting effects, single preparation method, and the inability to guarantee the sustained and slow release of sex pheromones. Enhancing the trapping effect and improving the slow-release time

Active Publication Date: 2021-02-26
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition and ratio of the sex pheromones of fall armyworm are different due to different geographical populations, and the commercialized sex attractants of fall armyworm have different attracting effects
Moreover, the preparation method is single, and the sustained release of sex pheromone cannot be guaranteed. Therefore, the present invention proposes a preparation method, using amphiphilic lipid molecules to load Spodoptera frugiperda sex pheromone and plant volatile linalool nano-loaded drug The construction of the system, the preparation of a sex attractant with sustained slow-release effect for the control of Spodoptera frugiperda

Method used

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  • Nano drug delivery system of grassland spodoptera litura sex attractant and application of nano drug delivery system
  • Nano drug delivery system of grassland spodoptera litura sex attractant and application of nano drug delivery system
  • Nano drug delivery system of grassland spodoptera litura sex attractant and application of nano drug delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Preparation of sex pheromones

[0020] Three components of sex pheromone (Z)-9-tetradecen-1-ol acetate, (Z)-7-dodecen-1-ol acetate and (Z)-11- Hexadecen-1-ol acetate is prepared according to the mass percentage of 90:0.5:9.5, and the solvent is tetrahydrofuran.

[0021] 2) Construction of nano drug-loading system for sex attractant of Spodoptera frugiperda

[0022] Take 1 mL of the mixed solution prepared in step 1) (the mass of the three-component mixed solution is 100 mg) and the synergist linalool 1 mg, and mix with 1 mg of the amphiphilic lipid molecule DSPE-PEG under ultrasound 5000 Mix, then pour 9 mL of distilled water and continue sonication for 120 s. Then the mixture was stirred overnight at room temperature, and the organic solvent was evaporated to obtain nanoparticles constructed by the nano drug-loading system.

[0023] 3) Preparation of sex lures for Spodoptera frugiperda

[0024] Take 100 μL of the Spodoptera frugiperda nanoparticles obtained in s...

Embodiment 2

[0026] The specific steps are the same as in Example 1: the synergist linalool in step 2) is not added in this step, and the others are all the same, and the lure number N2 of the gained.

Embodiment 3

[0028] Specific implementation steps:

[0029] Same as Example 1, step 1) Three different components of sex pheromones were prepared according to the mass percentage of 90:0.5:9.5, the mass of the mixed solution was 100 mg, diluted with n-hexane to 1 mL, and the mixed solution of sex pheromones was obtained;

[0030] Step 2) Take 10 μL of the sex pheromone mixture in 1) with a pipette gun, and slowly inject it into the polyvinyl chloride capillary, the lure is ready, numbered N3.

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Abstract

The invention discloses a nano drug delivery system of a grassland spodoptera litura sex attractant and application of the nano drug delivery system. The amphiphilic lipid molecule loaded sex pheromone and the synergist linalool are formed by self-assembly of amphiphilic lipid molecules DSPE-mPEG5000 (1, 2-distearoyl-sn-glycerin-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000]), sex pheromone and linalool through a cosolvent method, and then the amphiphilic lipid molecule loaded sex pheromone and the synergist linalool are adsorbed onto a sustained-release carrier 0.22 [mu]m polyether sulfone membrane. The sex pheromone is composed of (Z)-9-tetradecene-1-alcohol acetate, (Z)-7-dodecene-1-alcohol acetate and (Z)-11-hexadecene-1-alcohol acetate in a mass ratio of 90: 0.5: 9.5. Theinvention further discloses a moth luring effect of the moth luring agent as a grassland spodoptera litura sex luring agent in the field.

Description

technical field [0001] The invention belongs to the technical field of pest biological control, and in particular relates to a nanometer drug-carrying system of a sex lure of Spodoptera frugiperda and its application. Background technique [0002] Fall armyworm ( Spodoptera frugiperda ), belonging to the genus Spodoptera in the family Noctuidae of the order Lepidoptera, is reported to have originated from tropical and subtropical regions of the Americas and is a very destructive pest. It was found in Yunnan Province of my country in January 2019, and it had spread and invaded 18 provinces (cities, districts) in my country by May. Up to now, 27 provinces have been found to be invaded and damaged by F. frugiperda, and the trend continues to move northward. [0003] Fall armyworm is a polyphagous insect that can cause damage to various crops such as corn, rice, sorghum, wheat, sugar cane, cotton and rapeseed. Spodoptera frugiperda larvae have different preferences for differ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N49/00A01N37/02A01N25/10A01P19/00
CPCA01N25/10A01N37/02A01N49/00
Inventor 陈秀琴邱良妙刘其全何玉仙占志雄
Owner INST OF PLANT PROTECTION FAAS
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