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Method for preparing rhamnolipid by using semi-solid state fermentation method and application of rhamnolipid

A rhamnolipid and semi-solid technology, applied in the field of bioengineering, can solve the problems of defoaming difficulties, restrictions on the large-scale industrial application of rhamnolipids, and low yield of rhamnolipids, so as to save the cumbersome defoaming operation, promoting grain income and stable production, and promoting the effect of food safety

Inactive Publication Date: 2015-04-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although rhamnolipids have potentially broad applications, they are prone to foaming during fermentation and are difficult to control
Generally, rhamnolipid fermentation mainly uses oil as raw material. During the fermentation process, severe emulsification occurs between rhamnolipid and oil, which makes purification difficult.
When using glycerol as a substrate for fermentation, the yield of rhamnolipids is low
At present, there are two main enterprises in our country in production. The fermentation of rhamnolipids generally uses various vegetable oils as carbon sources. The fermentation cycle is long, the defoaming is difficult and the cost is high, and the cost of fermentation raw materials is also high. These all restrict the production of rhamnolipids. Large-scale industrial application of glycolipids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Preparation of rhamnolipids by semi-solid fermentation in shake flasks

[0015] Pseudomonas aeruginosa AB93066 was used as the starting strain for fermentation, and the medium included (g / L): rapeseed meal 100, bran 50, potassium dihydrogen phosphate 7, magnesium sulfate 0.3, sodium nitrate 6, and industrial glycerin 40. Put 50mL of tap water in a 250mL shaker flask, add the corresponding medium components to make a semi-solid fermentation medium, sterilize at 121°C for 15min, inoculate 6%, ferment in a shaker at 37°C at 100rpm for 12d, the yield of rhamnolipids Up to 19 g / kg. The fermented mash is pasteurized at 80°C for 30 minutes to obtain the product.

Embodiment 2

[0016] Example 2 Preparation of rhamnolipids by semi-solid fermentation in a fermenter

[0017] Pseudomonas aeruginosa AB93066 was used as the starting strain for fermentation, and the medium included (g / L): rapeseed meal 100, bran 40, crushed straw 20, potassium dihydrogen phosphate 7, magnesium sulfate 0.3, sodium nitrate 6, industrial Glycerin 40. Fill 3L tap water in a 7L fermenter, add the corresponding components of the medium, sterilize at 121°C for 15min, inoculum size 6%, ferment at 37°C, 150rpm for 12d, and the final rhamnolipid yield reaches 30 g / kg. The product can be obtained by steaming the fermented mash for 15 minutes to sterilize.

Embodiment 3

[0018] Example 3 Application of rhamnolipids in heavy metal contaminated soil remediation

[0019] The fermented mash containing rhamnolipids is diluted with water by 2 to 10 times of volume, so that the concentration of rhamnolipids in the solution is 1 g / L. Excavate lead and cadmium-contaminated soil from heavy metal-contaminated land, fill the soil in 1 kg portion for each flowerpot, and then add fermented mash solution containing rhamnolipid to rinse 4 times. Plant lettuce seedlings in flowerpots subsequently, after two months, measure the heavy metal content of soil and lettuce, compare with contrast, find that lead in the soil reduces from 153 mg / kg to 20 mg / kg, and cadmium content reduces from 51 mg to 20 mg / kg. 4 mg / kg, the lead content in lettuce was reduced from 3.6 mg / kg to 0.056 mg / kg, and the cadmium content was reduced from 2.1 mg / kg to 0.047 mg / kg.

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Abstract

The invention discloses a method for preparing rhamnolipid by using a semi-solid state fermentation method and application of the rhamnolipid and belongs to the technical field of bioengineering. The method disclosed by the invention comprises the following steps: by taking agricultural wastes such as rapeseed dregs and bran as raw materials, adding nutrient elements such as glycerin and phosphate, performing semi-solid state fermentation by utilizing pseudomonas aeruginosa AB93066 (purchased from CCTCC), thereby obtaining the rhamnolipid. The defoaming operation can be saved, and the obtained fermentation product containing the rhamnolipid can be directly applied to bioremediation of heavy metal contaminated soil after being sterilized. Meanwhile, the rhamnolipid has the effect of inhibiting pathogenic bacteria, and the soil organic matters are increased in the rapeseed dreg fertilized field.

Description

technical field [0001] The invention relates to a method for preparing rhamnolipid by a semi-solid fermentation method and an application thereof, belonging to the technical field of bioengineering. Background technique [0002] As of December 2014, more than 40% of the total area of ​​cultivated land in China has been degraded. The soil fertility has declined and compacted, and the thickness of humus has decreased, mainly relying on chemical fertilizers. It is said that 300 million mu of land in China is polluted by heavy metals. China supports 20% of the world's population with 7% of the world's arable land. The pressure is huge. Heavy metal polluted land seriously threatens my country's food security. [0003] Rhamnolipid is a biosurfactant with strong surface and interfacial activity, low toxicity and biodegradability. It is widely used in petroleum exploration, environmental protection, pharmaceutical and chemical industry, food and agriculture. Rhamnolipid is an anion...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44B09C1/02B09C1/08C12R1/385
Inventor 詹晓北吴剑荣朱莉郑志永
Owner JIANGNAN UNIV
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