Method for producing vitamin B12 by using Pseudomonas denitrificans based on pH value control

A Pseudomonas and vitamin technology, applied in the field of vitamin B12 production by Pseudomonas denitrification, can solve the problems of high cost, difficulty in purification, and many by-products, and achieve the effect of increasing production

Inactive Publication Date: 2018-11-30
GUANGJI PHARMA MENGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Production of vitamin B by chemical synthesis 12 The cost of ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A pH-controlled production of vitamin B by Pseudomonas denitrification 12 method, including the following steps:

[0022] 1) Add vitamin B 12 The production strain Pseudomonas denitrifica was inoculated into the fermentation medium and fermented at a temperature of 28°C. The composition of the fermentation medium was: sugarcane molasses 80g / L, yeast extract 100g / L, 5, 6-di Methylbenzimidazole 0.1g / L, diammonium hydrogen phosphate 0.8g / L, zinc sulfate 0.08g / L, calcium carbonate 1g / L, glycerophosphoric acid 0.6g / L, magnesium oxide 0.08g / L, cobalt chloride 0.2 g / L, urea 0.1g / L, betaine 20g / L, arginine 0.08g / L and thiamine 0.15g / L, the pH of the fermentation medium is 7.2; during 0~65 hours of fermentation, control dissolved oxygen The amount is 30mmol·L -1 h -1 , 66~180 hours of fermentation, control the dissolved oxygen to 20mmol·L -1 h -1 During the fermentation process, when the pH value is higher than 7, sugarcane molasses is added dropwise, and when the pH value...

Embodiment 2

[0027] A pH-controlled production of vitamin B by Pseudomonas denitrification 12 method, including the following steps:

[0028] 1) Add vitamin B 12The production strain Pseudomonas denitrifica was inoculated into the fermentation medium and fermented at a temperature of 25°C. The composition of the fermentation medium was: sugarcane molasses 75g / L, yeast extract 100g / L, 5, 6-di Methylbenzimidazole 0.08g / L, Diammonium Hydrogen Phosphate 0.5g / L, Zinc Sulfate 0.05g / L, Calcium Carbonate 0.8g / L, Glycerophosphate 0.5g / L, Magnesium Oxide 0.05g / L, Cobalt Chloride 0.15g / L, urea 0.1g / L, betaine 15g / L, arginine 0.05g / L and thiamine 0.1g / L, the pH of the fermentation medium was 7; The oxygen content is 25mmol·L -1 h -1 , 66~180 hours of fermentation, control the dissolved oxygen to 15mmol·L -1 h -1 During the fermentation process, when the pH value is higher than 7, sugarcane molasses is added dropwise, and when the pH value is lower than 7, urea is added dropwise to control the pH...

Embodiment 3

[0033] A pH-controlled production of vitamin B by Pseudomonas denitrification 12 method, including the following steps:

[0034] 1) Add vitamin B 12 The production strain Pseudomonas denitrifica was inoculated into the fermentation medium and fermented at a temperature of 30°C. The composition of the fermentation medium was: sugarcane molasses 80g / L, yeast extract 110g / L, 5, 6-di Methylbenzimidazole 0.15g / L, Diammonium Hydrogen Phosphate 1g / L, Zinc Sulfate 0.1g / L, Calcium Carbonate 1.5g / L, Glycerophosphate 0.8g / L, Magnesium Oxide 0.1g / L, Cobalt Chloride 0.3 g / L, urea 0.15g / L, betaine 25g / L, arginine 0.1g / L and thiamine 0.2g / L, the pH of the fermentation medium was 7.2. From 0 to 65 hours of fermentation, control the dissolved oxygen to 35mmol·L -1 h -1 , 66~180 hours of fermentation, control the dissolved oxygen to 20mmol·L -1 h -1 During the fermentation process, when the pH value is higher than 7, sugarcane molasses is added dropwise, and when the pH value is lower tha...

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Abstract

The invention belongs to the technical field of biological fermentation, and discloses a method for producing vitamin B12 by using Pseudomonas aeruginosa based on pH value control. The method for producing the vitamin B12 by using the Pseudomonas aeruginosa based on the pH value control comprises the steps that a production strain of vitamin B12, namely the Pseudomonas aeruginosa is inoculated into a fermentation medium, and fermentation is carried out at a temperature of 25-30 DEG C; the composition of the fermentation medium comprises 70-85g/L of cane molasses, 90-110g/L of yeast extract, 0.05-0.2 g/L of 5,6-dimethylbenzimidazole, 0.1-1g/L of glycerophosphoric acid, 0.05-2g/L of cobalt chloride, 0.05-0.2g/L of urea, 10-30g/L of betaine, 0.05-0.2 g/L of arginine, 0.01-0.5g/L of thiamine and the like; the pH value of fermentation broth is controlled at 7 by the cane molasses and the urea; and the purified vitamin B12 is separated from the fermentation broth. The yield of the vitamin B12 is improved by controlling the pH in the fermentation process.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a production of vitamin B by Pseudomonas denitrification based on pH value control 12 Methods. Background technique [0002] Vitamin B 12 Also known as cobalamin, it is an important nutritional factor for animals and humans. It is widely used in feed, food and medical and health fields. It has significant effects in many aspects, such as promoting the development and maturation of red blood cells, preventing pernicious anemia; maintaining Nervous system health; exists in the form of coenzymes, can increase the utilization rate of folic acid, promote the metabolism of carbohydrates, fats and proteins; can also promote protein synthesis, which plays an important role in the growth and development of infants; it can also eliminate restlessness, Concentrate, enhance memory and sense of balance, and vitamin B 12 Participate in the formation of a lipoprot...

Claims

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Application Information

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IPC IPC(8): C12P19/42C12N15/01C12N13/00C12R1/38
CPCC12N13/00C12N15/01C12P19/42
Inventor 张广勇刘延茂余菊杨辉
Owner GUANGJI PHARMA MENGZHOU
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