Method for producing vitamin B12 by using Pseudomonas denitrificans based on pH value control
A Pseudomonas and vitamin technology, applied in the field of vitamin B12 production by Pseudomonas denitrification, can solve the problems of high cost, difficulty in purification, and many by-products, and achieve the effect of increasing production
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Embodiment 1
[0021] A pH-controlled production of vitamin B by Pseudomonas denitrification 12 method, including the following steps:
[0022] 1) Add vitamin B 12 The production strain Pseudomonas denitrifica was inoculated into the fermentation medium and fermented at a temperature of 28°C. The composition of the fermentation medium was: sugarcane molasses 80g / L, yeast extract 100g / L, 5, 6-di Methylbenzimidazole 0.1g / L, diammonium hydrogen phosphate 0.8g / L, zinc sulfate 0.08g / L, calcium carbonate 1g / L, glycerophosphoric acid 0.6g / L, magnesium oxide 0.08g / L, cobalt chloride 0.2 g / L, urea 0.1g / L, betaine 20g / L, arginine 0.08g / L and thiamine 0.15g / L, the pH of the fermentation medium is 7.2; during 0~65 hours of fermentation, control dissolved oxygen The amount is 30mmol·L -1 h -1 , 66~180 hours of fermentation, control the dissolved oxygen to 20mmol·L -1 h -1 During the fermentation process, when the pH value is higher than 7, sugarcane molasses is added dropwise, and when the pH value...
Embodiment 2
[0027] A pH-controlled production of vitamin B by Pseudomonas denitrification 12 method, including the following steps:
[0028] 1) Add vitamin B 12The production strain Pseudomonas denitrifica was inoculated into the fermentation medium and fermented at a temperature of 25°C. The composition of the fermentation medium was: sugarcane molasses 75g / L, yeast extract 100g / L, 5, 6-di Methylbenzimidazole 0.08g / L, Diammonium Hydrogen Phosphate 0.5g / L, Zinc Sulfate 0.05g / L, Calcium Carbonate 0.8g / L, Glycerophosphate 0.5g / L, Magnesium Oxide 0.05g / L, Cobalt Chloride 0.15g / L, urea 0.1g / L, betaine 15g / L, arginine 0.05g / L and thiamine 0.1g / L, the pH of the fermentation medium was 7; The oxygen content is 25mmol·L -1 h -1 , 66~180 hours of fermentation, control the dissolved oxygen to 15mmol·L -1 h -1 During the fermentation process, when the pH value is higher than 7, sugarcane molasses is added dropwise, and when the pH value is lower than 7, urea is added dropwise to control the pH...
Embodiment 3
[0033] A pH-controlled production of vitamin B by Pseudomonas denitrification 12 method, including the following steps:
[0034] 1) Add vitamin B 12 The production strain Pseudomonas denitrifica was inoculated into the fermentation medium and fermented at a temperature of 30°C. The composition of the fermentation medium was: sugarcane molasses 80g / L, yeast extract 110g / L, 5, 6-di Methylbenzimidazole 0.15g / L, Diammonium Hydrogen Phosphate 1g / L, Zinc Sulfate 0.1g / L, Calcium Carbonate 1.5g / L, Glycerophosphate 0.8g / L, Magnesium Oxide 0.1g / L, Cobalt Chloride 0.3 g / L, urea 0.15g / L, betaine 25g / L, arginine 0.1g / L and thiamine 0.2g / L, the pH of the fermentation medium was 7.2. From 0 to 65 hours of fermentation, control the dissolved oxygen to 35mmol·L -1 h -1 , 66~180 hours of fermentation, control the dissolved oxygen to 20mmol·L -1 h -1 During the fermentation process, when the pH value is higher than 7, sugarcane molasses is added dropwise, and when the pH value is lower tha...
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