Method for simultaneously detecting residues of 64 veterinary drugs in aquatic products

A technology for aquatic products and residual drugs, applied in measuring devices, instruments, scientific instruments, etc., can solve the problem of inability to detect 4-nitroimidazole, etc., and achieve the effect of short cycle, high accuracy and good stability

Active Publication Date: 2018-03-20
INSPECTION AND QUARANTINE TECHNOLOGY CENTER ZHONGSHAN ENTRY EXIT INSPECTION AND QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The patent application with publication number 107167539A discloses a method for simultaneously determining 31 kinds of veterinary drug residues in fish, but this method can only detect 31 kinds of veterinary drugs at the same time, and cannot detect other veterinary drugs such as 4-nitroimidazole

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  • Method for simultaneously detecting residues of 64 veterinary drugs in aquatic products
  • Method for simultaneously detecting residues of 64 veterinary drugs in aquatic products
  • Method for simultaneously detecting residues of 64 veterinary drugs in aquatic products

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Embodiment 1

[0034]Qualitative and quantitative detection methods of 64 kinds of veterinary drugs in the aquatic products of the present invention in embodiment 1

[0035] 1. Detection method

[0036] (1) Sample pretreatment method

[0037] Take the sample to be tested and homogenize it. Accurately weigh (2.00±0.01) g of the homogenized sample and place it in a 50 mL polypropylene centrifuge tube, add 10 mL of acetonitrile-water (80:20, v / v) solution, vortex Rotate and mix for 30s, ultrasonically extract (sonic frequency 45kHz, effective power 200W) for 10min, add 4g of anhydrous sodium sulfate and shake for 10min, centrifuge at a speed of 4000r / min for 5min, take 5mL of the supernatant and concentrate it in a parallel quantitative concentrator until nearly dry , use 1mL of methanol-acetonitrile-water (2:2:6, V / V / V) containing 0.1% formic acid to make up the volume, add 4mL of acetonitrile and saturated n-hexane to vortex at 2000r / min for 30s, ultrasonic (sonic frequency 45kHz , effectiv...

Embodiment 2

[0057] Embodiment 2 Qualitative and quantitative detection methods of 64 kinds of veterinary drugs in aquatic products of the present invention

[0058] 1. Detection method

[0059] (1) Sample pretreatment method

[0060] Take the sample to be tested and homogenize it. Accurately weigh (2.00±0.01) g of the homogenized sample and place it in a 50 mL polypropylene centrifuge tube, add 10 mL of acetonitrile-water (80:20, v / v) solution, vortex Rotate and mix for 30s, ultrasonically extract (sonic frequency 45kHz, effective power 200W) for 10min, add 4g of anhydrous sodium sulfate and shake for 10min, centrifuge at a speed of 4000r / min for 5min, take 5mL of the supernatant and concentrate it in a parallel quantitative concentrator until nearly dry , use 1mL of methanol-acetonitrile-water (2:2:6, V / V / V) containing 0.1% formic acid to make up the volume, add 4mL of acetonitrile and saturated n-hexane to vortex at 2000r / min for 30s, ultrasonic (sonic frequency 45kHz , effective powe...

Embodiment 3

[0074] Embodiment 3 Qualitative and quantitative detection methods of 64 kinds of veterinary drugs in aquatic products of the present invention

[0075] 1. Detection method

[0076] (1) Sample pretreatment method

[0077]Take the sample to be tested and homogenize it. Accurately weigh (2.00±0.01) g of the homogenized sample and place it in a 50 mL polypropylene centrifuge tube, add 10 mL of acetonitrile-water (80:20, v / v) solution, vortex Rotate and mix for 30s, ultrasonically extract (sonic frequency 45kHz, effective power 200W) for 10min, add 4g of anhydrous sodium sulfate and shake for 10min, centrifuge at a speed of 4000r / min for 5min, take 5mL of the supernatant and concentrate it in a parallel quantitative concentrator until nearly dry , use 1mL of methanol-acetonitrile-water (2:2:6, V / V / V) containing 0.1% formic acid to make up the volume, add 4mL of acetonitrile and saturated n-hexane to vortex at 2000r / min for 30s, ultrasonic (sonic frequency 45kHz , effective power...

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Abstract

The invention discloses a method for simultaneously detecting residues of 64 veterinary drugs in aquatic products. The method comprises the following steps: performing pretreatment on samples so as towell reduce the interference effect of a substrate on a target compound; and detecting the treated samples by adopting ultra-high performance liquid chromatography-quadrupole rod/electrostatic fieldorbitrap high resolution mass spectrum. The method disclosed by the invention is capable of simultaneously detecting residue of 14 categories of 64 veterinary drugs in aquatic products. The method islow in detection cost, short in cycle, excellent in stability and high in accuracy and is applicable to daily rapid screening of the residues of the veterinary drugs in the aquatic products on a largescale.

Description

technical field [0001] The invention relates to a method for detecting residual medicine in aquatic products. Background technique [0002] In recent years, with the steady growth of disposable income, Chinese residents have paid more attention to nutrition and health in diet, and the demand for aquatic products has increased year by year, which has promoted the rapid development of the aquatic industry. However, in the production, transportation, market sales and catering links of aquatic products, in order to treat or prevent various diseases, the relevant personnel lack the corresponding scientific knowledge and the continuous pursuit of economic benefits in the process of using veterinary drugs, and there are misuse, abuse, prohibition or restrictions. Use of veterinary drugs. Food animals will develop drug resistance, affect animal disease control, and increase drug residues in animal foods. It poses a threat to the safety of animals and animal food. Long-term intake ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李蓉杨璐齐刘恭源罗阳丹
Owner INSPECTION AND QUARANTINE TECHNOLOGY CENTER ZHONGSHAN ENTRY EXIT INSPECTION AND QUARANTINE
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