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Construction method of cynomolgus macaque model for alcoholic liver disease

A technology of alcoholic liver disease and construction method, which is applied in the field of animal model construction, can solve the problems of lack of targeted treatment methods for alcoholic liver disease, and achieve good application prospects, short time and high success rate

Inactive Publication Date: 2017-02-15
FANGCHENGGANG CHANGCHUN BIOLOGICAL TECH DEV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] Clinically, the treatment of alcoholic liver disease lacks targeted treatment methods, and clinically only passively focus on symptomatic treatment (use nutritional support to prevent malnutrition and use glucocorticoids to control inflammation)

Method used

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  • Construction method of cynomolgus macaque model for alcoholic liver disease
  • Construction method of cynomolgus macaque model for alcoholic liver disease
  • Construction method of cynomolgus macaque model for alcoholic liver disease

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Embodiment Construction

[0026] The present invention will be described in further detail below in conjunction with specific examples.

[0027] A method for constructing an animal model of alcoholic liver disease, comprising the following steps:

[0028] (1) Male cynomolgus monkeys weighing 8-10 kg were selected as research objects. In addition to normal feeding of basal feed, they were allowed to drink drinking water containing aspartame during the first week of adaptation. Increase 10% edible ethanol every week and allow them to drink freely, screen out cynomolgus monkeys who drink more than 50ml of 30% alcohol every day at the end of the 4th as experimental animals; The mass percentage of spartame is 0.1-1%; the basic feed is 15-20% protein, 50-60% carbohydrate, 5% fat, 4-6% crude fiber, and 9-26% other;

[0029] (2) From the 5th week, 10% sucrose was used to replace aspartame in the alcohol solution, and in addition to continuing to feed 30% alcohol, an additional 20% pig suet was added to the ba...

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Abstract

The invention belongs to a construction method of an animal model, and in particular relates to a construction method of a cynomolgus macaque model for alcoholic liver disease. The construction method of the cynomolgus macaque model for alcoholic liver disease comprises the following steps: screening experimental animals, feeding the screened experimental animals with high-glucose and high-fat feed, and conducting liver biopsy and acquisition of the determined model. The construction method of the cynomolgus macaque model for alcoholic liver disease provided by the invention is short in duration and high in success rate; the construction method is suitable for researching the occurrence mechanism of the alcoholic liver disease and is convenient for screening drugs for treating the alcoholic liver disease and for evaluating the efficacy of the drugs on the alcoholic liver disease; and the construction method has a good application prospect.

Description

technical field [0001] The invention belongs to a method for constructing an animal model, and in particular relates to a method for constructing a cynomolgus monkey alcoholic liver disease model. Background technique [0002] With the rapid development of my country's economy, residents' alcohol consumption is also increasing, and problems related to drinking are becoming more and more prominent. A normal person can metabolize 120g of ethanol in 24 hours, but long-term, excessive drinking will exceed the metabolic capacity of the body, which can lead to alcoholic liver disease. The occurrence of this disease is mainly caused by the toxic effect of ethanol and its metabolite-acetaldehyde, especially the most obvious toxic effect of acetaldehyde on liver cells. The two directly or indirectly lead to a series of liver lesions: hepatocyte degeneration (alcoholic Fatty liver), necrosis (hepatitis), fibrosis (hepatic fibrosis), and can develop into liver cirrhosis and liver canc...

Claims

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Application Information

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IPC IPC(8): A61K31/02A61K49/00A01K67/02A23K50/00A23K20/163A23K20/105A23K20/158
CPCA01K67/02A61K31/02A61K49/0008
Inventor 阳明黄慧学黄卫宣韦毅黄冬梅韦富毅覃怀平
Owner FANGCHENGGANG CHANGCHUN BIOLOGICAL TECH DEV
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