Method for testing curative effect of Chinese medicine Zhibai Dihuang pill based on application of metabonomics

Abstract

The invention discloses a method for testing a curative effect of a Chinese medicine Zhibai Dihuang pill based on application of metabonomics. The method comprises the following steps: dividing experimental animals into a control group, a diabetic nephropathy model group and a diabetic nephropathy administration group, injecting the experimental animals and collecting tissue samples of urine, serum and kidney; fixing the renal tissues of the three groups of rats through 10% neutral formalin, carrying out paraffin embedding, slicing and flaking the renal tissues, staining the renal tissues through routine hematoxylin-eosin and observing the pathological changes of the renal tissues under an optical microscope; measuring the chemical indexes and detecting the histopathology of the serum samples and the urine samples; preparing tissue samples of urine, serum and kidney, and obtaining data of a 1H NMR (Nuclear Magnetic Resonance) spectrum; carrying out NMR data processing and pattern recognition analysis through a statistical method; and comparing and analyzing important physiological and biochemical indexes of different groups of rats. The method disclosed by the invention can be used for obtaining the information of a large amount of endogenous metabolites of an organism through a metabonomics technology and screening metabolite changes caused by effective ingredients in the Zhibai Dihuang pill.

Description

technical field [0001] The invention belongs to the technical field of drug research, in particular to a method for testing the curative effect of the traditional Chinese medicine Zhibai Dihuang Pills based on applied metabonomics. Background technique [0002] Zhibai Dihuang Pill (ZDP) is a commonly used Chinese patent medicine. It is composed of Liuwei Dihuang Pill (rehmannia glutinosa, cornus, yam, Alisma, Moutan bark and Poria cocos), which is a representative prescription for nourishing yin, plus Anemarrhena and Treated from Phellodendron Phellodendri, it strengthens the function of nourishing kidney, yin and clearing fire. In recent years, it has been reported that this drug can protect experimental animals from kidney damage caused by diabetic nephropathy. Clinical studies have also found that patients who took chemical drugs and Zhibai Dihuang Pills had more The therapeutic effect is obviously better than that of patients who use chemical drugs alone. However, due to...

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Problems solved by technology

[0006] The purpose of the embodiments of the present invention is to provide a test method based on the application of metabolomics for the efficacy of the traditional Chinese medicine Zhibai Dihuang Pills, which aims to solve the problem in the prior art that the various active ingredients in Zhibai Dihuang Pills cannot be measured and studied question

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