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Construction and evaluation methods of blood deficiency mouse model

A mouse model and evaluation method technology, which is applied in animal husbandry, material inspection products, instruments, etc., can solve the problems of low accuracy and poor specificity, and achieve strong specific effects

Active Publication Date: 2019-12-20
SHANXI UNIV
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Problems solved by technology

[0005] In order to solve the technical problems of low accuracy and poor specificity of the existing evaluation methods for blood deficiency models, the present invention provides an evaluation method for blood deficiency mouse models

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  • Construction and evaluation methods of blood deficiency mouse model
  • Construction and evaluation methods of blood deficiency mouse model
  • Construction and evaluation methods of blood deficiency mouse model

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Embodiment Construction

[0038] The implementation of the present invention will be described in detail below in conjunction with the accompanying drawings and examples, so as to fully understand and implement the process of how to apply technical means to solve technical problems and achieve technical effects in the present invention. It should be noted that, as long as there is no conflict, the embodiments of the present invention and various features in the embodiments can be combined with each other, and the formed technical solutions are all within the protection scope of the present invention.

[0039] A method for constructing a blood-deficiency mouse model in the present embodiment comprises the following steps:

[0040] On the first day, mice were subcutaneously injected with 20 mg / kg of acetophenhydrazine, on the morning of the fourth day, mice were subcutaneously injected with 10 mg / kg of acetophenhydrazine, and mice were intraperitoneally injected with 20 mg / kg of cyclophosphamide two hours...

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Abstract

The invention belongs to the technical field of evaluation methods of models, and particularly relates to construction and evaluation methods of a blood deficiency mouse model. The construction and evaluation methods mainly solve the technical problems that an existing evaluation method of a blood deficiency model is low in precision and poor in specificity. According to the construction and evaluation methods of the blood deficiency model, a metabonomic technology is adopted, the change of endogenous metabolites in the spleen organs of mice of a blank control group and a model group is analyzed, all mass spectrum spectrograms are processed through CD software to obtain integral data, then in combination with the content of 15 biomarkers, statistical analysis is made to the integral mean value change tendency of biomarkers in the spleen organs of the mice of the blank control group and the model group, so that the change tendency of the content of the metabolites of the spleen organs of the blood deficiency mice is obtained, and thus the blood deficiency mouse model is evaluated with pertinence. By means of the construction and evaluation methods, the rationality and scientificityof model replication are reflected, the profiles of mouse bodies of the blank control group and the model group are systematically and comprehensively shown, the methods have the advantages of being comprehensive, systematic, efficient and high in specificity, and a reliable evaluation method is provided for new drug research and development and pharmacological research.

Description

technical field [0001] The invention belongs to the technical field of model construction and evaluation methods, and in particular relates to a blood deficiency mouse model construction and evaluation method. Background technique [0002] Blood deficiency is a common clinical syndrome of traditional Chinese medicine, which refers to the pathological state in which the body's blood deficiency and nutritional function are low, resulting in insufficient nourishment of visceral tissues. Traditional Chinese medicine believes that the causes of blood deficiency mainly include excessive blood loss, or prolonged illness and depletion of yin and blood, or dysfunction of the spleen and stomach, and the inability of water and grain essence to metabolize blood. [0003] In modern pharmacological research, bloodletting, chemical injury, radiation injury and other means are often used to establish animal models of blood deficiency syndrome. At present, judging whether the blood deficien...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02G01N27/62G01N33/50
CPCA01K67/02G01N33/5091G01N27/62G01N2570/00A01K2207/20A01K2227/105A01K2267/0381
Inventor 秦雪梅李鑫琦刘月涛
Owner SHANXI UNIV
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