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Construction and application of elderly constipated animal model

An animal model and constipation technology, which can be used in compound screening/testing, preparations for in vivo experiments, pharmaceutical formulations, etc., can solve the problems of strong subjectivity of constipation models in clinical elderly constipation, poor simulation of constipation models, and poor flexibility , to achieve the effect of highlighting substantive features, strong repeatability and low cost

Active Publication Date: 2020-11-06
SHANXI UNIV
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Problems solved by technology

[0007] In order to solve the technical problems that the existing constipation model cannot better simulate the state of clinical senile constipation and the evaluation method of the constipation model is highly subjective, one-sided, and poor in flexibility, the present invention provides the construction and application of an animal model of senile constipation

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  • Construction and application of elderly constipated animal model
  • Construction and application of elderly constipated animal model
  • Construction and application of elderly constipated animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: a kind of construction method of senile constipation animal model, comprises the following steps:

[0063] Inject D-galactose solution 300 mg / kg subcutaneously at 8 a.m. every day, that is, the concentration is 300 mg / mL (0.1 mL / 100g). From the 7th week, white vinegar was administered at the first dose of 5 mL / rat, and white vinegar (3 mL / 200g) and 16.67 mol / L activated carbon ice-water mixture (5 mL / 200g) were alternately given to rats on single or double days, for 5 consecutive days. Aged constipation animal models were obtained after one week. The total modeling time is 11 weeks.

[0064] Rats in the blank control group were specifically constructed by subcutaneously injecting the same amount of normal saline as the model group at 8:00 a.m. every day. From the 7th week, the same amount of distilled water as in the model group was administered to the stomach, and the modeling time was 11 weeks in total.

Embodiment 2

[0065] Example 2: The established animal model of constipation in the elderly was verified, the metabolic profile was characterized by multivariate statistical analysis method, the metabolic profile analysis was performed on the data by principal component analysis, and the separation of data in each group was investigated.

[0066] The specific method is to collect the feces of the rats in the blank control group and the model group after the animal model is constructed, and conduct NMR analysis on them to obtain the NMR spectra of the rats in the blank control group and the model group. The results are shown in figure 1 with figure 2Then, multivariate statistical analysis is carried out to the nuclear magnetic spectrogram integration data matrix of the blank control group and the model group rats, and the contour map of the endogenous metabolites in the feces of the rats in the blank control group and the model group is obtained. The results are shown in image 3 ,from i...

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Abstract

The invention belongs to the technical field of model construction and verification, and provides construction and application of an elderly constipated animal model in order to solve the technical problems that the existing constipation model cannot simulate the state of clinical elderly constipation better and a constipation model evaluation method is strong in subjectivity, high in one-sidedness and poor in flexibility. The construction comprises steps as follows: mice in a model group are subjected to subcutaneous injection of D-galactose every day, by starting from the seventh week, whitevinegar is given by intragastric administration at the initial dose of 5 mL / mouse, and henceforth, the mice are fed with the white vinegar and an activated carbon and ice water mixture alternately according to odd and even days; and after five consecutive weeks, the elderly constipated animal model is obtained. With subcutaneous injection of D-galactose, senescence can be induced, with intragastric administration of the white vinegar and an activated carbon and ice water mixture, gastrointestinal motility changes and the constipation symptom appears, and the elderly constipated animal model is obtained by combination of the two factors. The elderly constipated animal model not only shows two features of senescence and constipation, but also can show characteristics of clinical elderly constipation, simulates the clinical constipation symptoms of the elderly, is applicable to traditional Chinese medicine screening for elderly constipation treatment and provides experimental evidence for related new drug research and development.

Description

technical field [0001] The invention belongs to the technical field of model construction and verification, and in particular relates to the construction and application of an elderly constipation animal model. Background technique [0002] Constipation (Constipation) is a common digestive tract disease in the elderly, which can occur alone, or as a concomitant symptom of certain diseases, or as an inducement for other diseases. The prevalence of chronic constipation increases with age. According to the survey, the incidence of constipation in the elderly aged 60 to 64 is 8.7%, and the incidence of constipation in the elderly aged ≥85 is as high as 19.5%. Chronic constipation that does not heal for a long time not only causes excessive mental stress in the elderly, but also is closely related to the occurrence of diseases such as lower gastrointestinal tumors, senile mental disorders, and colon cancer. Therefore, senile constipation is a potentially harmful disease, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/027A61K49/0008A01K2207/20A01K2227/105A01K2267/03
Inventor 刘晓节赵迪赵思俊李震宇张丽增秦雪梅
Owner SHANXI UNIV
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