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A method for simultaneous determination of chlorogenic acid and luteolin in honeysuckle

A technology of luteolin and honeysuckle is applied in the field of detection of active components of honeysuckle, and achieves the effects of high precision and accuracy, shortened extraction time and simple method

Inactive Publication Date: 2016-05-04
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

UPLC has been reported on the quantitative analysis of chlorogenic acid in honeysuckle, but how to quickly and accurately determine the content of chlorogenic acid and luteolin in honeysuckle remains to be studied

Method used

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  • A method for simultaneous determination of chlorogenic acid and luteolin in honeysuckle
  • A method for simultaneous determination of chlorogenic acid and luteolin in honeysuckle
  • A method for simultaneous determination of chlorogenic acid and luteolin in honeysuckle

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Effect test

Embodiment 1

[0028] The method for simultaneously measuring the content of chlorogenic acid and luteolin in Flos Lonicerae of the present invention, the instruments and materials used:

[0029] WatersAcquityUPLCH-Class system, including quaternary pump, vacuum online degasser, automatic sampler, PDA detector, Empower workstation. One-hundred-thousandth balance (Mettler); EL204 one-thousandth balance (Shanghai Precision Instrument Co., Ltd.); KQ-250DE ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0030] Methanol and acetonitrile (chromatographically pure, Fisher Company), ethanol, phosphoric acid (analytical pure, Beijing Chemical Plant), double distilled water. The reference substances chlorogenic acid (NO: 110753-201314) and luteolin (NO: 111720-201106) were purchased from China National Institutes for Food and Drug Control. The source of honeysuckle material is shown in Table 3. It was identified as the dried flower buds of Lonicera japonica Thunb. by Associate Researc...

Embodiment 2

[0042] The method for simultaneously measuring the content of chlorogenic acid and luteolin in Flos Lonicerae of the present invention comprises the following steps:

[0043] (1) Preparation of reference substance solution: Accurately weigh chlorogenic acid, luteolin reference substance: 0.889mg, 0.920mg, use acetonitrile-0.4% phosphoric acid aqueous solution as solvent, and dilute to 2mL in a brown volumetric flask as a reserve liquid. Other reference substance solutions with different mass concentrations were obtained by diluting the stock solutions.

[0044] (2) Preparation of the test solution: Take about 0.25 g of honeysuckle powder (through a 60-mesh sieve), accurately weigh it, place it in a stoppered conical flask, add 30 mL of 70% ethanol aqueous solution precisely, weigh it, and ultrasonically Extract (power 250W, frequency 40HZ) for 45min, let cool, supplement the reduced weight with 70% ethanol aqueous solution, shake well, filter, take appropriate amount of filtr...

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Abstract

The invention provides a method for simultaneously measuring the content of chlorogenic acid and luteolin in honeysuckle, which includes ultrasonic extraction and quantitative analysis, and the solid-liquid ratio of honeysuckle powder and 70% ethanol aqueous solution is 1:80-1 during ultrasonic extraction : 120, the extraction time is 45-60min. UPLC conditions are: Chromatographic column: Waters? Acquity? UPLC? BEH? RP18; mobile phase: acetonitrile-0.4% phosphoric acid water; gradient elution: 0-2min, 5%-16%B; 2-4min, 16%-20%B; 4-8min, 20%-50%B; 8 -10min, 50%-100%B; 10-11min, 100%-5%B; detection wavelength: 242nm. The method of the invention is simple, can simultaneously measure the contents of chlorogenic acid and luteolin in honeysuckle, has high sensitivity, high precision and accuracy, good repeatability, and is stable and reliable.

Description

technical field [0001] The invention relates to a detection method for effective components of honeysuckle, and more specifically relates to a method capable of quantitatively analyzing chlorogenic acid and luteolin, the active components of honeysuckle, at the same time. Background technique [0002] Honeysuckle is the dry flower bud or the first flower of Lonicera japonica Thunb., a traditional Chinese medicine, which has the effects of clearing away heat and detoxification, evacuating wind-heat, anti-virus, protecting the liver and promoting gallbladder. Honeysuckle is mainly produced in Henan, Shandong and other places, and its main chemical components are organic acids, triterpenoids, flavonoids and volatile oils, etc., among which organic acids are the active ingredients of honeysuckle antibacterial and antiviral. Chlorogenic acid is one of the main active ingredients of honeysuckle, not only as the quality control index of its original medicinal materials, but also th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 师凤华莫乔程赵祥升缪剑华刘凤鸣谭木秀蒲祖宁陈路
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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