Eosin staining solution and HE staining solution containing same

Abstract

The invention provides an eosin staining solution comprising the components of eosin, Biebrich scarlet, flame red, ethanol and water. The invention also provides a preparation method of the eosin staining solution, a HE staining solution containing the eosin staining solution and a staining method of the eosin staining solution. When the eosin staining solution provided by the invention is used for staining slices, the background is clear, the gradation is distinct, the staining effect is good, and the slices can be read easily, cannot fade easily and are convenient to store for a long time.

Description

technical field [0001] The invention relates to an in vitro diagnostic reagent, in particular to an eosin staining solution and a preparation method thereof, as well as an HE staining solution containing the eosin staining solution and a staining method thereof. Background technique [0002] Hematoxylin and eosin staining, referred to as HE staining, is the most widely used technique in histology, embryology, and pathology. HE staining is to immerse tissue sections or cell smears in hematoxylin staining solution and eosin staining solution respectively, and after appropriate time and treatment, the tissues, cells and other components are stained with different shades of color, resulting in different refractive indices. It is convenient for observation and research under an optical microscope. [0003] Hematoxylin is a kind of basic dye, which is extracted from logan and is oxidized to generate hematin. It is used together with mordant to make the chromatin in the nucleus an...

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Problems solved by technology

However, all of the above-mentioned staining solutions using eosin as a staining agent have the following problems: 1) the composition of the dye is single, resulting in unclear layers when staining the cytoplasm and extracellular matrix; 2) due to the low staining intensity of eosin , the above-mentioned eosin dyeing solution needs to add acetic acid as a dyeing accelerator to enhance the dyeing strength, but the addition of the dyeing accelerator will lead to the weakening of the dyeing color level, which further aggravates the problem that the dyeing level is not clear enough; at the same time, adding the dyeing accelerator After the treatment of the slices, acidic substances need to be washed not only during the subsequent processing of the slices, but also when the residual acidic substances are too much, it will easily fade during the preservation of the slices, and the obtained slices cannot be stored for a long time

[0012] The existing HE staining solution, that is, the hematoxylin and eosin staining solution, because the staining layer of the eosin staining solution is not clear and the coloring effect is not good, which in turn causes the background of the stained tissue section or cell smear to be blurred and difficult to read.

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