65 results about "Eosin Y" patented technology

The invention provides a catalyst for generating hydrogen by visible light photocatalytic reduction of water, and a preparation method thereof, wherein each 100mg of the catalysts contain 10-60mg of eosin-Y, 35-85mg of carbon nano tubes and 1-10mg of oxides. With the technical scheme of the invention, the eosin is used as a sensitizing agent, the carbon nano tubes are used as carriers and photogenerated electron channels, the oxides, such as copper oxide, are used as co-catalysts, so that hydrogen generation by visible light photocatalytic reduction of water is implemented. The catalyst has higher hydrogen generation activity under irradiation of the visible light, and can be used for generating hydrogen by visible light photocatalytic reduction of water. And the oxides substitute Pt to be used as the co-catalyst, thereby implementing non-platinum property of the catalyst.

The invention discloses a method for preparing a 2-iodine amyl -2-ene-1,4-diketone derivative by adopting visible light catalysis. The method comprises the steps of adding allene, perfluorobutyl iodide, an additive and a photocatalyst as shown in a formula (I) to an organic solvent at the molar ratio of 1:(1-2):(1-2):(0.005-0.1), stirring under the conditions of an oxygen atmosphere, visible light illumination, room temperature and ordinary pressure for 10-20h, carrying out post-treatment on a reaction liquid to obtain a 2-iodine amyl -2-ene-1,4-diketone derivative as shown in a formula (II), wherein the additive is NaI, NH4I or KI; the organic solvent is ethanol, dichloromethane, benzene, methylbenzene, tetrahydrofuran or acetonitrile; the photocatalyst is selected from one of the following components: Ir(ppy)3, Eosin Y, Rose Bengal, [Ir(dF-CF3-ppy)2(dtbbpy)]PF6, [Ir(ppy)2(tbbpy)]PF6, Ru(bpy)3(BF4)2. The method is mild in reaction condition, simple in operation, high in selectivity and good in yield; a substituent group can be expanded; and visible light catalysis is adopted, so that the method has the characteristics of being free of pollution and environment-friendly. The formulas are as shown in the specification.

The invention discloses a method for detecting melamine. The method comprises the following steps of: sequentially adding 0.05-5.0mL of 0.1-0.5g/L melamine solution, 1.0-2.5ml of 4.0*10<-6>mol/L eosin Y-solution, 0.5-1.0ml of 2.0*10<-4>mol/L neutral red solution and 0.3-1.0ml of Britton-Robinson buffer solution of which the pH value is 3.5 into a 10ml colorimetric tube, using the solution without melamine as the reagent blank, fixing the volume to a specific scale by using super-pure water, reacting for 30min in a water bath of 30 DEG C, subsequently respectively measuring the fluorescence value F538nm of the melamine-containing solution and the fluorescence value F0 of the reagent blank and calculating the difference value that deltaF538nm is equal to F538nm subtracted by the F0 value at 538nm on a fluorophotometer by using a 1cm quartz cuvette in a mode that 520nm is used as the excitation wavelength; preparing 30ml of milk, adding 20.0ml of 1% trichloroacetic acid solution and 8.0ml of 2% lead acetate solution, and carrying out ultrasound treatment for 20min; centrifuging a part of the solution for 10min, taking 1ml of the supernate, and measuring the fluorescence value and calculating the content of the melamine in the milk according to the same way. The method is simple, convenient and rapid.

A method of preparing cell samples for viable cell number quantification with a fluorescence digital imaging microscopy system employing digital thresholding technique. The cell sample is stained with a first, fluorescent dye and treated with a second dye that is able to quench the fluorescence of the first dye. The fluorescent dye accumulates in viable cells only and is used to stain the viable cells. The second dye is excluded from viable cells but enters non-viable cells, thereby quenching the background fluorescence in non-viable cells and the medium. Two examples of dye combinations are described: fluorescein diacetate used as the fluorescent dye with eosin Y as the quenching dye; and calcein-AM used as the fluorescent dye with trypan blue as the quenching dye. By reducing the background fluorescence, the dynamic range and accuracy of viable cell number measurements are enhanced. In low viability cultures treated with fluorescein diacetate, background fluorescence completely masked viable cells, but digital thresholding and eosin treatment dramatically reduced background fluorescence, producing a linear response over 4 logs of viable cell density.

The invention relates to a method for synthesizing a vinyl sulfone compound. The vinyl sulfone compound has a chemical molecular formula shown in the description, wherein R1 is one selected from H, a straight-chain alkyl electron-donating group, a nitro electron withdrawing group, F, Cl and Br, and R2, R3 and R4 each is selected from H and straight-chain alkyl. The synthetic method comprises the steps of adding an inorganic base in an organic reaction solvent by taking an aryl sulfonyl chloride compound and a tertiary amine compound as reaction substrates and taking eosin Y as a catalyst under the action of visible light, carrying out a reaction for 1 to 3 hours at the temperature of -5 DEG C to 5 DEG C under the irradiation of an LED (light-emitting diode) lamp, carrying out water washing treatment, extraction and drying after the reaction ends, then, carrying out reduced-pressure distillation to remove the organic solvent, and then, carrying out thin-layer chromatography and column chromatography separation, thereby preparing the vinyl sulfone compound. Compared with the prior art, the method provided by the invention has the advantages that the steps are simple, the reaction conditions are mild, the controllability is good, the consumption of metal complexes is avoided, the reaction cost is reduced, and the method is pollution-free to environments.

The invention relates to a light and thermal controlled nano supermolecule vesicle. In a construction unit of the light and thermal controlled nano supermolecule vesicle, sulphonated calyx arene is taken as a host, amphiphilic anthracene ether (AnPy) is taken as a guest, and a supramolecular assembly is constructed under the action of supermolecules. A preparation method of the light and thermal controlled nano supermolecule vesicle comprises the following steps: dissolving sulphonated calyx arene and amphiphilic anthracene ether in water respectively, and uniformly mixing to obtain light and thermal controlled nano supermolecule vesicle solution, wherein concentrations of sulphonated calyx arene and amphiphilic anthracene in the nano supermolecule vesicle solution are 0.04mM and 0.10mM respectively. The light and thermal controlled nano supermolecule vesicle has the advantages that the preparation method of the nano supermolecule vesicle is simple, and cost is low; the prepared supermolecule vesicle has good responsibility in a short time by means of external temperature and ultraviolet irradiation, and the supermolecule vesicle can be depolymerised by means of visible light irradiation after being mixed with a photodynamic therapy model drug Eosin Y, so that the light and thermal controlled nano supermolecule vesicle has a broad application prospect in the field of loading, transportation and controlled release of photodynamic therapy drugs.

The invention discloses a catalyst for hydrogen production and a preparation method thereof. In the catalyst for hydrogen production, eosin is used as a sensitizing agent, a carbon nano-tube is used as a carrier and a photoinduced electron channel, and a copper-based composite metal oxide is used as a catalyst promoter. The preparation method comprises the following steps of: mixing the carbon nano-tube and a metal saline solution, adding water, stirring for several hours, and evaporating water to dryness so as to obtain a carbon nano-tube and metal saline precursor; at the air atmosphere, calcining the precursor for hours so as to obtain a carbon nano-tube and copper-based composite metal oxide system; and soaking the carbon nano-tube and copper-based composite metal oxide system in an aqueous solution containing eosin Y under the protection of N2, and separating to obtain the catalyst for hydrogen production. Under the irradiation of visible light, the catalyst has higher hydrogen production activity and can be used for producing hydrogen by catalyzing and reducing water in visible light. The copper-based composite metal oxide can be used for replacing Pt to server as the catalyst promoter so that the non-platinization of the catalyst is realized; and therefore, the catalyst has a certain application value in the aspect of hydrogen production by reducing water through photocatalysis.

A 3,3'-diindolyl acetate compound and a derivative thereof are discovered to have extensive activities of immunity resistance, inflammation resistance, edema inhibition and the like. Meanwhile, The 3,3'-diindolyl acetate compound is also an important intermediate raw material which belongs to the field of organic preparation. The compound can be used for synthesizing multiple bioactive molecules with 3,3'-bis-indolymethane structure, and has quite high organic synthesis application value. The invention provides a quite simple preparation method of a visible light catalyzed 3,3'-diindolyl acetate compound. The preparation method comprises the following specific steps: taking a glycine derivative and an indole derivative as raw materials, taking an organic photosensitizer (eosin Y, rose-bengal or rhodamine 6G) as a photocatalyst, reacting for 10-30 h at the room temperature under illumination of visible light, and separating and purifying to obtain a product. Compared with the prior art,the preparation method has the advantages of simple steps, high yield, cheap and easily obtained raw materials and catalysts, zero metal, mild reaction condition, simplicity in reaction operation andthe like, and is more suitable for pilot scale-up and large-scale preparation of the 3,3'-diindolyl acetate compound.

The present document describes a screening composition comprising a marker compound, chosen from at least one of iodine, and fluorescein; eosin Y, erythrosine, ponceau S, calcein, a catalyst, chosen from at least one boron trioxide (B₂O₃), potassium (K), Gallium (III) oxide (Ga₂O₃), Nickel (II) oxide (NiO), Vanadium (V) oxide (V₂O₅), magnesium oxide (MgO), a bismuth oxide chosen from bismuth subcarbonate [Bi₂O₂(CO₃)], bismuth chloride oxide (BiClO), and bismuth oxide (Bi₂O₃), cesium bromide (CsBr), lanthanum (III) oxide (La₂O₃), molybdenum (VI) oxide (MoO₃), neodymium oxide (Nd₂O₃), Nickel (II) carbonate anhydrous (NiCO₃); and a pigment, chosen from at least one of scandium (III) oxide (Sc₂O₃), Lead (IV) oxide (PbO₂), Sulfur (S) powder, and Tungsten (VI) oxide (WO₃), chromium (III) oxide (Cr₂O₃), copper (II) oxide (CuO), copper (I) oxide (Cu₂O), iron (III) oxide (Fe₂O₃), lead (II) oxide (PbO). The document also describes method of using the same.

Disclosed are a method for detecting proteins on a polyacrylamide gel by background staining method using an organic dye composition containing eosin Y or phloxine B, and the organic dye composition for use in the method, which enables the rapid and simple detection of the protein on the polyacrylamide gel with a high sensitivity.

Systems and methods of polymerizing compositions with a light source may be provided. A device may include a handheld light source, which may include an LED, which may provide light at limited wavelengths to polymerize a composition. The device may be used to photo-polymerize dermal fillers transdermally via ex vivo applications. Dermal fillers may be compositions including PEODA 3400 and eosin Y, which may be polymerizable when exposed to light.

The invention discloses a reagent kit for screening early-stage cervical cancers based on cervical liquid-based cytology and DNA methylation. The reagent kit comprises orange-G6, brilliant green liquid, Bismarck brown liquid, eosin Y liquid, a QlAamp DNA Mini Kit reagent kit body, a QIAamp EpiTect Bisulfite Kits reagent kit body and PCR reaction liquid, the forward primer sequence of JAM3-M4 is shown as SEQ ID No.6, the reverse primer sequence of JAM3-M4 is shown as SEQ ID No.7, the forward primer sequence of beta-actin is shown as SEQ ID No.16, the reverse primer sequence of beta-actin is shown as SEQ ID No.17. According to the reagent kit for screening the early-stage cervical cancers, detection flexibility, specificity, the positive predictive value and the negative predictive value meet the requirements of clinical diagnosis through primary verification of clinical tissue samples and further verification of a large amount of cervical exfoliated cytology.

The invention discloses a preparation method of a high-conductivity graphene-eosin Y composite aerosol photocatalyst. The high-conductivity graphene-eosin Y composite aerosol photocatalyst is preparedby: adding certain high-conductivity Elicarb graphene into graphene oxide-eosin Y solution, performing hydrothermal treatment, and lyophilizing. When exposed to visible light, high-conductivity graphene-eosin Y composite aerosol exhibits excellent photocatalytic activity in liquid photocatalytic reduction nitrobenzene and Cr (VI); the preparation method is simple and effective to perform and hasgood practical value and promising application prospect in the preparation of more effective graphene-aerosol composite photocatalysts.

The invention discloses anti-stacking nitrogen doped three-dimensional graphene and a preparation method thereof. The preparation method comprises the following steps: hydrothermally preparing anti-stacking graphene oxide by using melamine, a formaldehyde aqueous solution with a concentration of 37wt% and a graphene oxide aqueous solution; and calcining the prepared anti-stacking graphene oxide under a nitrogen atmosphere so as to be able to obtain the anti-stacking nitrogen doped three-dimensional graphene. According to the invention, the catalyst has good eosin Y sensitized photocatalytic water-splitting hydrogen production activity after loaded with precious metal PT with a mass ratio of 10% under illumination of visible light; and the preparation method is simple and practicable, and the catalyst is cheap and can be conveniently recycled, so the composite photocatalic material has extensive practicable values and application prospects in the technical files of material preparation and the field of photocatalytic water-splitting hydrogen production.

The invention belongs to the field of organic synthesis, and particularly relates to a preparation method of indolizine cyclo-1, 2-diketone with fluorescence activity and a derivative thereof. The indolizine cyclo-1, 2-diketone is prepared by adopting a new metal-free photocatalytic oxidation method, and the indolizine cyclo-1, 2-diketone is synthesized from commercially available indolizine, 2-oxo-2-phenylacetaldehyde, rose bengal and other basic raw materials, wherein the groups R1, R2 and R3 are alkyl, an electron withdrawing group or an electron donating group; rose bengal can also be replaced with a photocatalyst eosin Y, eosin B, rhodamine 6G or fluorescein; the solvent dimethyl sulfoxide can also be replaced with an organic solvent N, N-dimethylformamide, acetonitrile or dichloromethane. The method has the characteristics of easily available starting materials, wide substrate application range, economy, high efficiency, mild conditions, environmental friendliness and high yield.

The invention discloses a Diff-Quik dye liquor special for animals and a preparation method of the Diff-Quik dye liquor. The Diff-Quik dye liquor comprises a first reagent, a second reagent and a third reagent; the first reagent contains the following components: solid green and methanol; the second reagent contains the following components: eosin Y, monopotassium phosphate, disodium hydrogen phosphate dodecahydrate and ultrapure water; and the third reagent contains the following components: methylene blue, azure II, azure I, eosin Y, monopotassium phosphate, disodium hydrogen phosphate dodecahydrate, potassium permanganate and ultrapure water. The Diff-Quik dye liquor is suitable for animal blood cell smears, the staining color is very clear, the staining background is free of sediment,the color is bright, the distinction degree is high after staining of different cells, and the application range is wider; compared with the existing Diff-Quik dye liquor, the Diff-Quik dye liquor disclosed by the invention has more advantages in dyeing time, and dyeing can be generally completed within 30 to 60 seconds. And the Diff-Quik dye liquor disclosed by the invention is extremely short inpreparation time, so that the production benefit is remarkably improved.

The invention discloses a method of synthesizing 8-hydroxyquinoline. Ortho-aminophenol and acrolein are used as raw materials, and hydroquinone is used as a polymerization inhibitor; an addition reaction and a cyclization reaction are carried out under the acidic condition to obtain an intermediate, and a cobalt oxime chelate photocatalyst and an eosin Y photosensitizer are directly added withoutthe need of separation of the intermediate; and an acetonitrile solvent and deionized water are added, under continuous irradiation of visible light, the 8-hydroxyquinoline is obtained through a photocatalytic dehydrogenation oxidation reaction. The reaction process is easy to control, by-products are few, post-treatment is simple, and the generated target product is good in selectivity, high in yield and stable.

The invention belongs to the technical field of organic synthesis, and particularly discloses a preparing method of a 2-substituted-1,3-dithiane derivative. The preparing method comprises the following steps of adding substrate aldehyde, 1,3-dimercaptopropane and catalytic amount Eosin Y into a proper amount of acetonitrile, continuously stirring the mixture, using a blue LED to irradiate the mixture for 3 h at a room-temperature open system, after the reaction is finished, removing organic solvents through rotary evaporation, and then obtaining a product through recrystallization and chromatography. According to the preparing method of the 2-substituted-1,3-dithiane derivative, the yield of the product is high, the conversion rate of the substrate is high, the reaction operation conditions are mild and environmentally friendly, demanding water and oxygen removal operations and devices are not needed, and the reaction can be conducted at the room temperature.

The invention relates to the field of electrochemical analysis, in particular to a method for determining the hydroquinone in the wastewater by the poly eosin Y modified glassy carbon electrode. The eosin Y (EY) is modified to the surface of the glassy carbon electrode (GCE) by electropolymerization method, and a poly eosin Y modified electrode (pEY/GCE) is prepared, which has good electrocatalytic effect on hydroquinone. The electrochemical behavior of hydroquinone on the modified electrode was studied by cyclic voltammetry (CV). In a pH=5 acetate buffer solution, the concentration of hydroquinone showed a good linear relationship with its redox peak current in the range of 5.0x10-10~1.8x10-6mol/L, and the minimum detection concentration is 1.25x10-10mol/L.