Eosin Y based electrochemiluminescence sensor

An electrochemistry and sensor technology, applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, analysis by making materials undergo chemical reactions, etc.

Inactive Publication Date: 2016-09-07
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Until now, electrochemically pretreated glassy carbon electrodes have not been directly applied in electrochemiluminescence studies.

Method used

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  • Eosin Y based electrochemiluminescence sensor
  • Eosin Y based electrochemiluminescence sensor
  • Eosin Y based electrochemiluminescence sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Polishing of glassy carbon electrode: The glassy carbon electrode is first mechanically ground and polished on the chamois covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning, 2 minutes each time, repeated three times , until cleaned, and finally washed thoroughly with ethanol, dilute acid and water in sequence;

[0040](2) The three-electrode system was used for the test, with the bare glassy carbon electrode as the working electrode, the platinum wire electrode as the counter electrode, and the Ag / AgCl as the reference electrode. voltage to obtain the cyclic voltammetry curve of the bare glassy carbon electrode in pH 10.0 phosphate buffer solution without eosin Y (e.g. figure 1 shown in a); the above three-electrode insert containing 1 × 10 -4 The pH 10.0 phosphate buffer solution of mol / L eosin Y was applied, and the cyclic voltammetry voltage was appli...

Embodiment 2

[0042] (1) Polishing of glassy carbon electrode: The glassy carbon electrode is first mechanically ground and polished on the chamois covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning, 2 minutes each time, repeated three times , until cleaned, and finally washed thoroughly with ethanol, dilute acid and water in sequence;

[0043] (2) Preparation of oxidized glassy carbon electrode: The clean glassy carbon electrode was moved into a 0.1 mol / L sulfuric acid solution, and the surface of the glassy carbon electrode was electrochemically pretreated to obtain an oxidized glassy carbon electrode. The oxidized glassy carbon electrode was rinsed with secondary water and stored in pH 7.0 phosphate buffer solution for use;

[0044] (3) The three-electrode system was used for the test. The oxidized glassy carbon electrode was used as the working electrode, the platinum wire elect...

Embodiment 3

[0046] (1) Polishing of glassy carbon electrode: The glassy carbon electrode is first mechanically ground and polished on the chamois covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning, 2 minutes each time, repeated three times , until cleaned, and finally washed thoroughly with ethanol, dilute acid and water in sequence;

[0047] (2) Preparation of oxidized glassy carbon electrode: The clean glassy carbon electrode was moved into a 0.1 mol / L sulfuric acid solution, and the surface of the glassy carbon electrode was electrochemically pretreated to obtain an oxidized glassy carbon electrode. The oxidized glassy carbon electrode was rinsed with secondary water and stored in pH 7.0 phosphate buffer solution for use;

[0048] (3) The three-electrode system was used for the test. The bare glassy carbon electrode was used as the working electrode, the platinum wire electrode...

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Abstract

The invention discloses an eosin Y based electrochemiluminescence sensor. In the sensor, an oxidized glass-carbon electrode is taken as the work electrode, eosin Y is taken as the electrochemiluminescence substance, a phosphoric acid buffer solution is taken as the electrolyte buffer system, and thus high sensitive sensing of bioactive molecules such as hydrogen peroxide, guanine, adenine, NADH, uric acid, vitamin C, and the like, is realized. A glass-carbon electrode is processed by an electrochemical method to obtain the oxidized glass-carbon electrode. Under the assistance of the oxidized glass-carbon electrode, eosin Y can give off sensitive and stable electrochemiluminescence radiation signals in the cathode electrochemiluminescence channel (-0.5V). Under same conditions, eosin Y has higher luminescence efficiency, compared with conventional luminescent reagents; and moreover, the sensing system can detect a plurality of bioactive substances high sensitively. The electrochemiluminescence sensor has the advantages of simple preparation, green, environment-friendliness, and low cost, and has a certain application prospect in the field of biomedicine.

Description

technical field [0001] The invention belongs to the technical field of electrochemiluminescence analysis, in particular to an electrochemiluminescence sensor based on eosin Y. Background technique [0002] According to the reaction principle, electrochemiluminescence (ECL) can be roughly divided into two types: ion quenching type and co-reactant type. Compared with ion-quenched electrochemiluminescence, co-reactant electrochemiluminescence shows more advantages such as stronger electrochemiluminescence and wider applicability of various solvents, etc. Most of the chemiluminescence studies are co-reactive electrochemiluminescence. Due to its high sensitivity and simple operation process, co-reactant-type EL systems have been widely used in pharmaceutical analysis, environmental analysis and clinical detection and other analytical applications. Commonly used co-reactive EL luminescent reagents, including ruthenium bipyridine, luminol and lucigenin, have been extensively and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N21/76
CPCG01N27/48G01N21/76
Inventor 林燕语戴宏张书培
Owner FUJIAN NORMAL UNIV
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