Hematoxylin-eosin mixed staining solution

A technology of hematoxylin and eosin and staining solution, which is applied in the field of dyeing, sample processing reagents and its preparation, can solve the problems of unclear layers, complicated operation, false blue, etc., and achieve clear layers, uniform dyeing, and clear coloring Effect

Active Publication Date: 2016-06-08
安徽雷根生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] In order to solve the traditional hematoxylin and eosin dyeing operation complex, time-consuming, false blue, unclear layers, the present invention "a hematoxylin and eosin mixed dyeing solution" aims to provide a hematoxylin and eosin mixed together, just A new type of staining solution that can stain both hematoxylin and eosin when staining cells, tissues and other samples once

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Embodiment 1

[0054] "A hematoxylin and eosin mixed staining solution" of the present invention, the preferred components of each 1000ml staining solution for paraffin section dyeing are: hematoxylin 5g, alcohol-soluble eosin 1.0g, absolute ethanol 250ml, aluminum potassium sulfate 50g, glycerine 150ml of alcohol, 0.4g of sodium iodate, 5g of water-soluble eosin, 0.5g of flamingo, 10.6ml of glacial acetic acid, 0.1g of formaldehyde, and the rest is deionized water, a total of 1000ml.

[0055] Preparation method of hematoxylin and eosin mixed staining solution for paraffin section:

[0056] Preparation of alcohol-soluble reagent: take alcohol (250ml) according to the selected volume fraction, add hematoxylin (5g), alcohol-soluble eosin (1.0g), glycerol (150ml) according to the selected ratio, fully dissolve, and obtain alcohol Dissolving reagent, spare.

[0057] Prepare the mordant reagent: take water (600ml) according to the selected volume fraction, add aluminum potassium sulfate (50g) ac...

Embodiment 2

[0065] "A hematoxylin-eosin mixed staining solution" of the present invention, the preferred components of every 1000ml staining solution for frozen section staining are: hematoxylin 3.5g, alcohol-soluble eosin 1.0g, absolute ethanol 250ml, aluminum potassium sulfate 30g, acetone 150ml of triol, 0.3g of sodium iodate, 3g of water-soluble eosin, 0.4g of flamingo, 9.6ml of glacial acetic acid, 0.1g of formaldehyde, and the rest is deionized water, a total of 1000ml.

[0066] Preparation method of hematoxylin and eosin mixed staining solution for frozen sections:

[0067] Preparation of alcohol-soluble reagent: take alcohol (250ml) according to the selected volume fraction, add hematoxylin (3.5g), alcohol-soluble eosin (1.0g), and glycerol (150ml) according to the selected ratio, fully dissolve, and obtain Alcohol-soluble reagent, spare.

[0068] Preparation of mordant reagent: Take water (600ml) according to the selected volume fraction, add aluminum potassium sulfate (30g) acc...

Embodiment 3

[0076]"A hematoxylin and eosin mixed staining solution" of the present invention, the preferred components of each 1000ml staining solution for cell staining are: hematoxylin 2.6g, alcohol-soluble eosin 1.7g, absolute ethanol 250ml, aluminum potassium sulfate 25g, glycerine Alcohol 150ml, sodium iodate 0.22g, water-soluble eosin 0.5g, flame red 0.2g, glacial acetic acid 10ml, formaldehyde 0.1g, the rest is deionized water, a total of 1000ml.

[0077] Preparation method of hematoxylin and eosin mixed staining solution for cell staining:

[0078] Preparation of alcohol-soluble reagent: take alcohol (250ml) according to the selected volume fraction and add hematoxylin (2.6g), alcohol-soluble eosin (1.7g) and glycerol (150ml) according to the selected proportion, fully dissolve, and obtain Alcohol-soluble reagent, spare.

[0079] Prepare the mordant reagent: take water (600ml) according to the selected volume fraction, add aluminum potassium sulfate (25g) according to the selecte...

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Abstract

The invention provides a hematoxylin-eosin mixed staining solution, which is characterized by comprising the following components: hematoxylin, an oxidant, a mordant, eosin, alcohol, weak acid, a preservative and water. The novel staining solution is prepared by mixing hematoxylin and eosin together; and cell and tissue samples only need one-time staining for staining both the hematoxylin and eosin. The present invention also provides a preparation method and a staining method of the hematoxylin-eosin mixed staining solution, and solves the problems of complicated operation, time consumption, false blue, unclear hierarchy and short retention time on the traditional hematoxylin-eosin staining.

Description

Technical field: [0001] The invention belongs to the field of biomedicine, and in particular relates to a sample treatment reagent for one-step dyeing after hematoxylin and eosin is mixed, and a preparation and dyeing method thereof. Background technique: [0002] Hematoxylin (Hematoxylin) and Eosin (also known as eosin) combined staining, referred to as HE staining, is the most basic staining method in routine pathological slide preparation, and is widely used. In pathological diagnosis, teaching and scientific research, HE staining is often used to observe the morphology and structure of normal and diseased tissues. To determine or identify certain abnormal substances and special components in diseased tissues and cells, the special staining methods, enzyme histochemical methods, and immunohistochemical methods that need to be used are also based on the observation of HE-stained tissue sections. of. In HE-stained tissue sections, the nuclei are blue and the cytoplasm is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 刘涛王红郭喜常建元张立明段学明
Owner 安徽雷根生物技术有限公司
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