Application of eosin and derivative of eosin in protein detection

A protein detection and derivative technology, used in the preparation of test samples, color/spectral property measurement, etc., can solve the problems of poor mass spectrometry compatibility, cumbersome operation, low sensitivity, etc., achieving good reproducibility, simple and rapid operation, The effect of less environmental pollution

Inactive Publication Date: 2012-07-18
温州安得森生物科技有限公司
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  • Abstract
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Problems solved by technology

Although many new staining techniques have been developed in the past 30 to 40 years, there are still one or several disadvantages as follo

Method used

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  • Application of eosin and derivative of eosin in protein detection
  • Application of eosin and derivative of eosin in protein detection
  • Application of eosin and derivative of eosin in protein detection

Examples

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Embodiment 1

[0025] Example 1 Negative staining of eosin protein

[0026] figure 1 is the chemical structural formula of eosin.

[0027] Figure 2-4 The eosin protein negative staining experiment was performed using the following steps:

[0028] 1) Fix the protein sample (SDS6H2) gel after SDS-PAGE electrophoresis in 50% methanol / 10% acetic acid solution for 30 min. Then wash with deionized water twice, each time for 5 minutes;

[0029] 2) Equilibrate in 50% methanol solution for 10 minutes;

[0030] 3) Dye in the staining solution for 15 minutes, then rinse in deionized water for 3 minutes. The staining solution is an aqueous solution containing 0.5% (W / V) eosin and 50% methanol / 1% acetic acid;

[0031] 4) After the gel was stained, it was scanned under the Epson V700 scanner in the reverse scanning mode.

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Abstract

The invention relates to the technique of protein negative staining detection, in particular to an application of an eosin and a derivative of the eosin in protein negative staining detection. A method using the eosin to perform protein negative staining detection is further provided, which comprises the steps of fixing a gel of a protein sample after electrophoresis in a stationary liquid, washing with deionized water, performing equilibrium in a methanol solution, staining and developing. The application of the eosin and the derivative of the eosin in protein detection have the advantages of being high in sensitivity, simple and quick in operation, good in reproducibility, linear relation and mass spectrum compatibility, safe in usage, low in cost, applicable to the research of high throughput proteomics and the like.

Description

technical field [0001] The invention relates to protein negative stain detection technology, in particular to a new negative dye for protein detection. Background technique [0002] In proteomics research, gel protein staining technology is a key technology connecting two-dimensional electrophoresis and downstream mass spectrometry analysis. The current state-of-the-art mass spectrometers are capable of analyzing trace proteins at the pg level. Conventional gel protein staining techniques include Coomassie brilliant blue staining, silver staining, and fluorescent staining. The Coomassie Brilliant Blue staining method has low sensitivity, the silver staining method is complicated to operate, has poor compatibility with mass spectrometry, and the fluorescent staining method is expensive. These factors seriously restrict the development of high-throughput proteomics. Although many new staining techniques have been developed in the past 30 to 40 years, there are still one or ...

Claims

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Application Information

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IPC IPC(8): G01N21/25G01N1/30
Inventor 金利泰丛维涛
Owner 温州安得森生物科技有限公司
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