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Preparation method of biocompatible gamma-polyglutamic-acid hydrogel

A biocompatible, polyglutamic acid technology, applied in the field of biocompatible γ-polyglutamic acid hydrogel preparation, can solve the problem of reducing gel hydrophilicity and swelling properties, narrow molar ratio conversion range, The problem of low swelling rate of the gel system can reduce the generation of rearrangement by-products, improve biocompatibility, and achieve good biocompatibility.

Active Publication Date: 2012-01-18
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The resulting gel system prepared by this method has a porous structure and is biodegradable, but the swelling rate of the obtained gel system is too low (1.65-3.19), and the conversion range of the molar ratio of the two substances is relatively narrow, and the main component is still Oligochitosan (accounting for about 80%-99%) reduces the hydrophilicity and swelling properties of the gel, limiting its application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: polyglutamic acid: chitooligosaccharide: the molar ratio of EDC: NHS is 2: 1: 2: 2 system: polyglutamic acid 100mg, chitooligosaccharide 71.71mg, dissolve in 2ml deionized water, stir to form After the homogeneous solution system was added, 148.6 mg of EDC and 89.2 mg of NHS were added, ice-bathed at 4 ° C for 10 min, and then left at room temperature for 3 h to form a hydrogel. The prepared hydrogel was purified by dialysis in deionized water, swelled to equilibrium, and freeze-dried. The swelling ratio of the obtained gel was 132.37 g / g.

Embodiment 2

[0013] Embodiment 2: polyglutamic acid: chitosan oligosaccharide: the molar ratio of EDC: NHS is 4: 1: 4: 4 system: polyglutamic acid 100mg, chitosan oligosaccharide 47.79mg, dissolve in 2ml deionized water, stir to form After the homogeneous solution system was added, 148.6 mg of EDC and 89.2 mg of NHS were added, ice-bathed at 4 ° C for 10 min, and then left at room temperature for 3 h to form a hydrogel. The prepared hydrogel was purified by dialysis in deionized water, swelled to equilibrium, and freeze-dried. The swelling rate of the obtained gel was 260.29 g / g.

Embodiment 3

[0014] Embodiment 3: polyglutamic acid: chitosan oligosaccharide: the molar ratio of EDC: NHS is 2: 1: 0.5: 0.5 system: polyglutamic acid 100mg, chitosan oligosaccharide 71.71mg, dissolve in 2ml deionized water, stir to form After the homogeneous solution system was added, 37.15 mg of EDC and 22.3 mg of NHS were added, ice bathed at 4 °C for 10 min, and then left at room temperature for 3 h to form a hydrogel. The prepared hydrogel was dialyzed and purified in deionized water, swelled and balanced, and dried in vacuum. The swelling rate of the obtained gel was 36 g / g.

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Abstract

The invention discloses a preparation method of biocompatible gamma-polyglutamic-acid hydrogel, which comprises the following steps of: by using polyglutamic acid as a main raw material and a biological-source material chitosan oligosaccharide as a cross-linking agent, carrying out preparation reaction in an aqueous solution, and activating a gamma-polyglutamic-acid carboxyl group by utilizing activating agents diamine carbide and N-hydroxysuccinimide ester to enable the polyglutamic acid and the free amino group of the chitosan oligosaccharide to form an amido bond to further form the hydrogel in a three-dimensional netted structure. After the prepared gel is swelled and balanced, drying is carried out by adopting a freeze-drying, vacuum-drying or microwave-drying mode. The biological-source material chitosan oligosaccharide is selected as the cross-linking agent, so that the biocompatibility of an obtained hydrogel system can be markedly improved; and a reaction system is an aqueous-solution system, reaction regulating and controlling properties are good, and the gel is stable. Because the hydrogel prepared by the method disclosed by the invention has good biocompatibility, the hydrogel can be widely applied to medicament carriers, auxiliary wound materials, medical and sanitary articles and the like.

Description

Technical field: [0001] The invention relates to a preparation method of biocompatible hydrogel, in particular to a preparation method of biocompatible gamma-polyglutamic acid (gamma-PGA) hydrogel obtained by chemical cross-linking. Background technique: [0002] Previous studies have shown that the preparation methods of polyglutamic acid hydrogel are mainly physical cross-linking method and chemical cross-linking method. Among them, the gel preparation process by physical radiation cross-linking method is simple, no need to add cross-linking agent, and the product does not need to be processed and purified. However, the entire radiation process requires nitrogen or vacuum protection. In addition, there are potential safety hazards in the use and storage of radiation sources. The preparation of polyglutamic acid hydrogel by chemical cross-linking is more flexible due to different systems, but the cross-linking agent selected by the existing chemical method for preparing po...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/24C08L77/04C08L5/08C08K5/17C08K5/3415
Inventor 王淑芳洪彦航张明睿宋存江李岩冬解慧陈思原
Owner NANKAI UNIV
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