Surface treatment method of tissue-engineered bioactive implant

Abstract

A surface treatment method of a tissue-engineered bioactive implant comprises the following steps of: (1) obtaining marrow stromal cells from autologous ilium or obtaining periodontal ligament stem cells from a fresh healthy tooth which is pulled off; (2) putting the marrow stromal cells or the periodontal ligament stem cells into a culture dish in which Alpha-MEM culture medium is added, and adding factors promoting the secretion of extracellular matrix into the marrow stromal cells or the periodontal ligament stem cells so as to form marrow stromal cell aggregate or periodontal ligament stemcell aggregate; (3) wrapping an implant with the marrow stromal cell aggregate or the periodontal ligament stem cell aggregate; (4) placing the wrapped implant in the culture medium; and (5) adding vitamin C and hexadecadrol into the culture medium to promote the secretion of the extracellular matrix, thereby causing the marrow stromal cell aggregate or the periodontal ligament stem cell aggregate together with the implant to form tissue-engineered implant with bioactivity. The surface treatment method solves the problem that patients suffering from osteoporosis and diabetes and recovering from radiation treatment have low implant success rate.

Description

technical field [0001] The invention relates to a medical method for a craniomaxillofacial implant, in particular to a method for treating the surface of a tissue engineered bioactive implant. Background technique [0002] Pluripotent stem cells with multidirectional differentiation and tissue regeneration capabilities, as seed cells for tissue engineering, have achieved good results in promoting tissue repair and treating systemic diseases, and have gradually been widely used and developed. Cell aggregate technology is a new technology for constructing regenerative tissue using seed cells in tissue engineering. Using this technology, a large number of seed cells can be compounded on suitable scaffold materials to construct tissue engineered tissues in vitro. At present, the existing craniofacial implants mainly include titanium, titanium alloy and zirconium ceramic materials, which are processed by surface physics (acid-etching sandblasting), chemical (micro-arc oxidation) ...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a surface treatment method for tissue-engineered bioactive implants, which can replace implant processing for special patients, so that implants can obtain osseointegration in special implant areas, and solve the problem of implant placement in hosts. The problem of easy vascularization and osseointegration of bone tissue in the region lays the foundation for the later restoration of implant dentures and maxillofacial defects

Images