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Two-layer composite periodontal defect repair material based on photo-crosslinking hydrogel and preparing method of material

A double-layer composite and repair material technology, applied in medical science, prosthesis, etc., can solve the problems of single composition, structure and function, complicated operation and use, complicated preparation process, etc., and achieve good biocompatibility, excellent mechanical Performance and stability, the effect of high hydrophilicity

Active Publication Date: 2019-06-14
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the current technical difficulty is that the existing single-layer periodontal defect repair materials have problems such as single composition, structure and function; while the preparation process of double-layer periodontal defect repair materials is usually cumbersome, and the operation is complicated.

Method used

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  • Two-layer composite periodontal defect repair material based on photo-crosslinking hydrogel and preparing method of material
  • Two-layer composite periodontal defect repair material based on photo-crosslinking hydrogel and preparing method of material
  • Two-layer composite periodontal defect repair material based on photo-crosslinking hydrogel and preparing method of material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]Preparation of GelMA: Weigh 10.0 g of pigskin collagen with a balance, add it to 100 mL of deionized water, heat to 60°C, and stir until the collagen is completely dissolved. Add 8 mL of methacrylate solution (methacrylicanhydride, MA) to the above collagen solution at a flow rate of 0.5 mL / min and a temperature of 80° C., stir, and let stand for 3 hours after all the MA solution is added dropwise. Subsequently, 500 mL of deionized water was added, stirred for 30 minutes, and then packed in a dialysis bag with a molecular weight of 12,000. Place the subpackaged dialysis bags in deionized water, magnetically stir, and dialyze; replace the deionized water every 24 hours for 7 days. The dialyzed GelMA solution was filtered, placed in a large Petri dish, covered with a parafilm, and kept overnight at -80°C. Finally, the pre-frozen GelMA solution was placed in a low-temperature freeze dryer, freeze-dried for 5 days, and the samples were taken out and stored in the dark.

Embodiment 2

[0039] Preparation of GelMA electrospun fibers: Weigh 1g of GelMA with a balance, add 5mL, 8mL, 10mL, and 12mL of hexafluoroisopropanol solution, shake until the GelMA is completely dissolved, and obtain different concentrations of GelMA / hexafluoroisopropanol solutions. Take a certain concentration of the above solution into a 5mL syringe, and fix the syringe to the syringe pump of the electrospinning machine. Cut a 30cm x 30cm rectangular piece of aluminum foil and wrap it around the receiver. The distance between the needle of the syringe and the foil is 10cm. The needle is connected to the positive electrode, the aluminum foil is connected to the negative electrode, the voltage of the spinning machine is set to 22kv, and the driving speed of the syringe pump is 0.0015mm / s. After electrospinning until all the GelMA solution was exhausted, the spun GelMA fiber membrane was removed from the electrospinning machine and left at room temperature for 24 hours to evaporate all the ...

Embodiment 3

[0042] Preparation of GelMA fiber hydrogel membrane: The GelMA electrospun fibers with different fiber diameters prepared in Example 2 were cut into square samples (size: 1 cm*1 cm). Mix 10 mL of absolute ethanol with 1 mL of dihydroxydimethylpropiophenone solution and place on a shaker for 10 minutes to obtain a cross-linking liquid. Take 0.1ml of cross-linking solution and add it dropwise to each GelMA electrospun fiber sample in turn, and light it for 90s with a point-type cross-linking instrument (OmniCure, Canada) at a light intensity of 2.9mW / cm 2 , with a distance of 1 cm to prepare the GelMA fiber hydrogel membrane. In order to remove the residual cross-linking agent, the cross-linked samples were soaked in absolute ethanol for 5 h, and then used.

[0043] Periodontal ligament stem cells were sequentially seeded on GelMA fiber hydrogel membranes with different fiber diameters (cell density: 10 6 per well), using osteogenic induction medium (containing 10mM β-glycerop...

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Abstract

The invention discloses a two-layer composite periodontal defect repair material based on photo-crosslinking hydrogel and a preparing method of the material. The periodontal defect repair material isprepared with biomacromolecules GelMa and synthetic macromolecules PEGDMA which both have photo-crosslinking features. The two-layer composite hydrogel material can integrate the advantages of a GelMAfiber hydrogel layer and the advantages of a PEGDMA hydrogel layer, so that the two-layer composite hydrogel material has the shapes of three-dimensional fibers and communicated pores, good biocompatibility, high hydrophilicity and excellent mechanical performance and stability. The GelMA fiber hydrogel layer can regulate and control differentiation of periodontal ligament stem cells towards osteoblasts through fiber size on the condition that no growth factor is added, and tissue regeneration of the alveolar bone is effectively promoted; the PEGDMA hydrogel layer can effectively stop soft tissue (gingival tissue) from invading a defect area, and an effective space is provided for the tissue regeneration of the alveolar bone; in the meanwhile, the transportation of nutrient substances isnot influenced while the stopping effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of preparation of periodontal defect repair materials, in particular to a double-layer composite periodontal defect repair material based on photocrosslinked hydrogel and a preparation method thereof. Background technique [0002] Periodontal disease is one of the most common oral diseases in humans. It is a chronic inflammatory disease that destroys the supporting tissues around teeth. The national oral health epidemiological survey report shows that the number of adults suffering from different degrees of periodontal disease in my country accounts for more than 80% of the total population of the country. After suffering from periodontal disease, the gums are congested and edematous, and the alveolar bone is destroyed and its height is reduced, thereby forming a deep periodontal pocket between the tooth and the tooth root, making the periodontal ligament unable to attach to the normal alveolar bone. It can...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/22A61L27/18A61L27/50A61L27/52A61L27/56
Inventor 马玉菲徐峰李昂
Owner XI AN JIAOTONG UNIV
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