Periodontal ligament stem cells osteogenic differentiation inducing liquid and method

Abstract

The invention relates to a periodontal ligament stem cells (PDLSCs) osteogenic differentiation inducing liquid and a method. The inducing liquid is prepared from the following components: basal culture medium, vitamin C, dexamethasone, beta-glycerophosphate sodium, FBS, IGF-1 and BMP-2. The periodontal ligament stem cell osteogenic differentiation inducing method uses the above osteogenic differentiation inducing liquid to induce. According to the multiple inducing factor osteogenic inducing liquid provided by the invention, multiple inducing factors are jointly applied in PDLSCs, so that the PDLSCs osteogenic differentiation can be effectively induced. According to the invention, the adopted raw materials are third molars required to be removed when orthodontic treatment is carried out on 12-18-year-old teenagers, and at present, the third molars are basically adopted as medical wastes to throw away; while according to the invention, the third molars are used for isolated culturing the PDLSCs to carry out osteogenic induction, so that the recycle of the medical wastes is effectively realized.

Description

technical field [0001] The invention relates to the field of induction and differentiation of stem cells, in particular to a solution for inducing osteogenic differentiation of periodontal ligament stem cells and a method thereof. Background technique [0002] Periodontal tissue is the tissue around the tooth, also known as the supporting tissue of the tooth, including alveolar bone, gingiva, periodontal ligament and cementum. Periodontal disease refers to diseases that occur in the periodontal tissue, including gingival disease that only involves the gingival tissue and periodontitis that affects the deep periodontal tissue (periodontal ligament, alveolar bone, cementum). Periodontal disease is a common oral disease, one of the main causes of adult tooth loss, and a major oral disease that endangers human teeth and general health. The early symptoms of periodontal disease are not easy to pay attention to, resulting in long-term chronic infection of periodontal tissue, and ...

AI Technical Summary

Problems solved by technology

[0009] At present, the research on the induction of osteogenic differentiation of PDLSCs mainly focuses on the different effects of different cytokines on their biological behavior, but is often limited to the research on the effect of a single cytokine

Since the organism is a complex organism with multiple inducing factors, in the complex microenvironment of periodontal tissue, various inducing factors may play a synergistic or antagonistic role in the process of periodontal regeneration. uncertain

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