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Periodontal ligament stem cell adipogenic differentiation inducing liquid and periodontal ligament stem cell adipogenic differentiation inducing method

A technology of periodontal ligament stem cells and adipogenic differentiation, applied in the direction of non-embryonic pluripotent stem cells, animal cells, vertebrate cells, etc., to achieve the effects of less damage to the human body, realization of reuse, and high-efficiency adipogenic induction potential

Active Publication Date: 2016-04-06
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, the induction medium and method for inducing the adipogenic differentiation of periodontal ligament stem cells need to be further improved.

Method used

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  • Periodontal ligament stem cell adipogenic differentiation inducing liquid and periodontal ligament stem cell adipogenic differentiation inducing method
  • Periodontal ligament stem cell adipogenic differentiation inducing liquid and periodontal ligament stem cell adipogenic differentiation inducing method
  • Periodontal ligament stem cell adipogenic differentiation inducing liquid and periodontal ligament stem cell adipogenic differentiation inducing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A periodontal ligament stem cell adipogenic differentiation induction solution, comprising the following components in the following contents: 0.1mmoL / LIBMX, 10mg / L insulin, 0.1mmoL / L indomethacin, 1μmoL / L dexamethasone, 1v / L v%PRP, 10v / v%FBS, and the balance is DMEM / F12 medium.

[0044] In this example, PRP was prepared by the following method: transfer the peripheral blood from the same donor as the tooth into a 15mL centrifuge tube in an ultra-clean bench, 10mL per tube, 400g, and centrifuge for 10min. After centrifugation, the blood sample was divided into three layers from top to bottom: light yellow supernatant layer, white cell layer and red cell layer. Aspirate all supernatant layer, white cell layer and upper 1-2mm of red cell layer, transfer to new 15mL centrifuge tube, centrifuge at 400g for 5min. After centrifugation, absorb the supernatant layer and white cell layer, transfer to a new 15mL centrifuge tube, and centrifuge at 1200g for 5min. Discard the sup...

Embodiment 2

[0058] This example is basically the same as Example 1, only the adipogenic differentiation induction medium used is different. The adipogenic differentiation induction medium used in this embodiment contains the following components in the following contents: 0.1mmoL / LIBMX, 10mg / L insulin, 0.1mmoL / L indomethacin, 1μmoL / L dexamethasone, 5v / L v%PRP, 10v / v%FBS, and the balance is DMEM / F12 medium.

Embodiment 3

[0060] This example is basically the same as Example 1, only the adipogenic differentiation induction medium used is different. The adipogenic differentiation induction medium used in this embodiment contains the following components in the following contents: 0.1mmoL / LIBMX, 10mg / L insulin, 0.1mmoL / L indomethacin, 1μmoL / L dexamethasone, 10v / L v%PRP, 10v / v%FBS, and the balance is DMEM / F12 medium.

[0061] In order to verify the effect of osteogenic differentiation induction of the present invention, adipogenic differentiation induction is carried out with the induction solution and method in Example 1-3 as the experimental group 1-3, and a negative control group and a positive control group are set at the same time, and the experimental group and the control group Oil red O staining, expression of peroxisome proliferator-activated receptor γ-2 (PPARγ-2) and lipoprotein lipase (LPL) were detected. Each control group is basically the same as that of Example 1, except for the adi...

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Abstract

The invention relates to periodontal ligament stem cell adipogenic differentiation inducing liquid and a periodontal ligament stem cell adipogenic differentiation inducing method. The adipogenic differentiation inducing liquid is prepared from a basic culture medium, IBMX, insulin, indometacin, dexamethasone, PRP and FBS; preferentially, the adipogenic differentiation inducing liquid is prepared from, 0.1 mmoL / L of IBMX, 10 mg / L of insulin, 0.1 mmoL / L of indometacin, 1 micromoL / L of dexamethasone, 5 v / v% of PRP, 10 v / v% of FBS and the balance basic culture medium, wherein the basic culture medium is a DMEM / F12 culture medium. The adipogenic differentiation inducing method comprises the step of performing inducing through the adipogenic differentiation inducing liquid. On the basis of common inducing culture liquid, PRP is added; in this way, the pimelosis efficiency of PDLSCs is effectively improved, and adipogenic inducing of PDLSCs can be achieved successfully.

Description

technical field [0001] The invention relates to the field of induction and differentiation of stem cells, in particular to a solution and method for inducing adipogenic differentiation of periodontal ligament stem cells. Background technique [0002] Periodontal ligament is a layer of connective tissue between the tooth root and alveolar bone, and is an important periodontal support tissue, mainly composed of fibroblasts, cementoblasts and some undifferentiated mesenchymal cells. Under normal circumstances, periodontal tissue has its own ability to renew and repair, and its repair activity is realized through the self-renewal of periodontal ligament cells. During disease or when receiving external stimuli, the tissue is regenerated through the continuous proliferation and differentiation of cells in the periodontal ligament. In 2004, Seo et al isolated periodontal ligament stem cells (PeriodontalLigamentSemCells, PDLSCs) from the periodontal ligament tissue of the extracted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074C12N5/077
Inventor 陈海佳王一飞葛啸虎戚康艺
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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